Hyalurosmooth. by Beauty Creations. Natural fine line and wrinkle filler

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1 Hyalurosmooth by Beauty Creations Natural fine line and wrinkle filler

2 Hyalurosmooth Botanical alternative to hyaluronic acid Smoothing and filling of fine lines and wrinkles by injecting «fillers» such as hyaluronic acid (e.g. Restylane 1 ), has become increasingly popular. For those who d rather avoid needles or who prefer a more natural and «green» approach, there is an alternative that can be topically applied. is a natural, botanically derived high molecular weight polysaccharide from the seeds of the Indian plant Cassia angustifolia. has a dual activity. The polysaccharides form a protective film on the skin that strongly binds water and fills up fine lines. By providing intense moisturization the skin is additionally plumped up, which further adds to reducing fine lines and wrinkles and smoothing of the skin surface. As a result of the smoother surface, light reflection is also improved, leading to increased skin radiance. In a comparative study was shown to be more effective than or as effective as sodium hyaluronate at equivalent concentrations of active matter. also has excellent sensorial properties. Skin immediately feels smooth, soft and silky. is perfectly suited as botanical alternative to hyaluronic acid, both for fine line filling and intense moisturization. In addition, it enhances skin radiance and provides immediately perceivable sensorial benefits. 1 trademark of HA North American Sales AB

3 Definition / Composition The active fraction of is extracted and purified from the seeds of a renewing Indian leguminous plant: Cassia angustifolia. is a stable, concentrated colloidal hydrosolute, ready for use made of a native botanical heteropolysaccharide, with a high molecular weight (~ 170,000 Daltons). can be considered as an equivalent of hyaluronic acid, because of its branched structure as well as its functions, especially its water attracting properties, and thereby, skin moisturizing and smoothing. In addition, it gives some exceptional benefits to sensorial and optical properties of the skin. Skin benefits Due to its excellent water binding, film forming and surface smoothing properties, provides: - intense moisturization, - reduction of fine lines and wrinkles, - smoothing of the skin surface, - increased skin radiance. Cosmetics use - Moisturizing face and eye care. - Skin plumping face care for reduction of fine lines and wrinkles. - Radiance enhancing face care. - Moisturizing body care to reduce ashiness. - Long term, 24 hr moisturizing care. - Botanical alternative to hyaluronic acid or sodium hyaluronate. Dosage / Solubility / Mode of incorporation 1. Dose of use: LS 8998: 2.5 to 5%. PW LS 8997: % 2. Solubility: dispersible in water, insoluble in ethanol and oils. 3. Mode of incorporation: LS 8998 is incorporated into the cosmetic product at room temperature for gels and tonics, and below 50 C, during the finishing process for emulsions. PW LS 8997 can be incorporated into the hot water phase of emulsions up to 80 C, or dissolved into water at room temperature for cold processing. Optimal ph : 4-9 Analytical characteristics 1. Aspect: LS 8998 Light beige fluid gel with a weak odor. PW LS 8997 White to beige powder with a weak odor. 2. Specifications: upon request. Storage In its original packaging, at C. INCI name LS 8998 Water (and) Cassia Angustifolia Seed Polysaccharide. PW LS 8997 Cassia Angustifolia Seed Polysaccharide.

4 Moisturizing activity on human stratum corneum (dielectric conductivity) Aim To evaluate the moisturizing activity on stratum corneum in vitro of 2.5% and 5% in a hydrogel referring to hydrogel with 0.07% and 0.20% sodium (Na) hyaluronate, by measurement of the dielectric conductivity. Human skin Stratum corneum 44% relative humidity Control without treatment Conductivity measurement before treatment Placebo hydrogel Treatment Hydrogel Hydrogel containing containing 0.07% Na 0.20% Na hyaluronate hyaluronate Hydrogel containing 2.5% Hydrogel containing 5% Dose 1 mg/cm² 3 times in 60 minutes (0, 30 and 60 minutes) Conductivity measurement up to 24 hours Results Fig. 1 Schema of protocol. % increase of dielectric conductivity (active product versus placebo) Sodium hyaluronate Before 0.5h 1h 2h 4h 6h 24h Before 0.5h 1h 2h 4h 6h 24h Duration Duration Hydrogels containing: 0.07% Na hyaluronate 0.20% Na hyaluronate 2.5% 5% Statistics: Percentage calculated on the basis of the mean from 10 experiments Mann-Whitney s U test Significant / control Significant / control and placebo 2.5% and 5% = 0.07% and 0.20% hyaluronic acid in terms of active matter Fig. 2 Moisturizing activity of evaluated by measurement of dielectric conductivity on the human horny layer in vitro at 44% relative humidity. Conclusion At equivalent concentrations 2 of active matter, provided equivalent moisturization as the benchmark, sodium hyaluronate, up to 6 hours. outperformed the benchmark with respect to the long term effect and gave significant 24 hours moisturization % and 5% LS 8998 correspond to 0.07% and 0.20% sodium hyaluronate respectively in terms of active matter.

5 Improvement of skin complexion (clinical test) Aim To evaluate in vivo the perception of the benefits on skin complexion of a treatment with a cream containing 3% versus placebo cream and cream containing 0.06% sodium (Na) hyaluronate. The skin complexion is a global parameter. Its evaluation considers simultaneously the skin clarity, color, eveness, texture. All theses properties contribute to the good appearance, i.e. smooth skin and radiant complexion. 12 trained panelists being used to describe and assess the sensory efficacy of cosmetic products, according to standardized gestures Products tested on the face in double blind with randomization Evaluation of skin complexion according to a scale (1=not at all to 7=very intense) before application and 3, 15 and 30 minutes after application Fig. 3 Schema of protocol. Results Improvement of skin complexion (Ti-T0) / Pl ( ) / Na / Pl / Na / Pl / Na Placebo cream (Pl) Cream containing 0.06% Na hyaluronate (Na) Cream containing 3% T3 T15 Minutes T30 T0 = before treatment Ti = T3, T15 and T30 = 3, 15, 30 minutes after treatment Statistics: Mean ± SEM of 12 volunteers Wilcoxon s T test ( ) Close to significancy p<0.01 Fig. 4 Sensorial evaluation of skin complexion by 12 trained panelists. Conclusion The cream containing 3% has significantly improved the skin complexion compared to the cream containing 0.06% Na hyaluronate and to placebo cream.

6 Sensorial effects (clinical test) Aim To evaluate the improvement of global sensorial skin properties with a cream containing 10% versus placebo cream. 15 female volunteers with a very dry and squamous skin Placebo cream Cream containing 10% A single application by the volunteer (double blind) Sensorial analysis Sensation of Suppleness - Softness - Moisturization before, immediately and 45 minutes after application Fig. 5 Schema of protocol. Results None Gentle Important Softness Suppleness Moisturization Ta T0 Ta T45 Ta T % (NS) % +16.1% (NS) % % (NS) % % scale of self-evaluation Statistics: Percentage calculated on the basis of the mean from 15 volunteers Wilcoxon s T test (NS) Not significant p=0.02 p=0.01 Ta = before treatment T0 = immediately after application T45 = 45 minutes after application Placebo cream Cream containing 10% Fig. 6 Sensorial evaluation of three skin benefits, after single topical application. Conclusion The cream containing 10% has clearly and significantly improved (versus placebo) the overall qualities of skin: softness, suppleness and moisturization. General conclusion Due to its excellent water binding, film forming and surface smoothing properties, Hyalurosmooth TM provides: - immediate moisturization (comparable to sodium hyaluronate), - 24 hours moisturization, - increased skin radiance, - skin softness, smoothness. Hyalurosmooth TM is perfectly suited as botanical alternative to hyaluronic acid, both for fine line filling and intense moisturization.

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8 EUROPE BASF Beauty Creations 49, avenue Georges Pompidou Levallois-Perret Cedex FRANCE Tel: +33 (0) Fax: +33 (0) AMERICAS BASF Corporation 361 Sheep Pasture Road East Setauket, NY USA Tel: +1 (631) JAPAN & ASIA-PACIFIC BASF Japan Ltd. 21F Roppongi Hills Mori Tower, Roppongi, Minato-ku, Tokyo, JAPAN Tel: +81 (0) Fax: +81 (0) Edition march, 2012 Although all statements and information in this publication are believed to be accurate and reliable, they are presented gratis and for guidance only, and risks and liability for results obtained by use of the products or application of the suggestions described are assumed by the user. SELLER MAKES NO WARRANTY OF ANY KIND, EITHER EXPRESS OR IMPLIED, BY FACT OR LAW, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Statements or suggestions concerning possible use of the products are made without representation or warranty that any such use is free of patent infringement and are not recommendations to infringe any patent. The user should not assume that toxicity data and safety measures are indicated or that other measures may not be required. The claims and supporting data provided in this publication have not been evaluated for compliance with any jurisdiction s regulatory requirements and the results reported may not be generally true under other conditions or in other matrices. Users must evaluate what claims and information are appropriate and comply with a jurisdiction s regulatory requirements. Recipient of this publication agrees to (i) indemnify and hold harmless each entity of the BASF organization for any and all regulatory action arising from recipient s use of any claims or information in this publication, including, but not limited to, use in advertising and finished product label claims, and (ii) not present this publication as evidence of finished product claim substantiation to any regulatory authority.

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