Product data sheet TRIspire Enhance

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1 Product data sheet TRIspire Enhance A Natural Enhancer for Skin Actives Extracted from the Larch tree harvested in North America, TRIspire Enhance (INCI: Galactoarabinan) is a highly functional polysaccharide. This natural polysaccharide, galactoarabinan (GA), is a natural polymer linked with sugar units consisting of galactose and arabinose in the ratio of 6:1, respectively. Unlike many other natural products, TRIspire Enhance displays a synthetic like consistency, characterized in part by its narrowly peaked molecular weight distribution and high intrinsic purity. This natural polymer has found many unique properties and benefits in personal care applications. It also imparts multifunctionality to water based systems. TRIspire Enhance is a natural, mild, non irritating and water soluble polymer which enhances emulsion stability, reduces transepidermal water loss (TEWL), and provides moisture control in formulations. This product also promotes AHA exfoliation on skin without irritation, could improve skin superficial fine lines, provides film forming and skin tightening, improves the uniformity and spreadability of a formulation and provides SPF enhancement. Active Matter INCI Name: Chemical Name: Botanic Name: Empirical Formula: Molecular Weight: TSCA CAS NO: ENEICS No: Galactoarabinan Arabinogalactan Genus Larix [(C 5 H 8 O 4 )(C 6 H 10 O 5 ) 6 ] x ~20,000 Daltons D Galacto L Arabinan Galactoarabinan Physical and Chemical Properties Appearance: Solvent Compatibility: Granular, white free flowing powder Galactoarabinan is extremely soluble in water and proportionally soluble in many solvents: Table 1. Solvent Compatibility at 10% TRIspire Enhance Solvent % Solvent in H 2 O Ethanol 30% Methanol 30% Isopropanol 30% Acetone 30% DMSO 50%

2 Rheology Property and Electrolyte Stability: Galactoarabinan has minimal contribution to the formulation viscosity. It also has minimal interference with the formulation ph, salt contents, ionic species, as well as shear. Specifications Assay > 95% Galactoarabinan Physical State: Texture Free Flowing Powder Foreign Matter None Odor Slightly Aromatic Color Off White/Tan Particle Size: < 20% +40M Moisture: 2 6 % Viscosity (5% solution): < 5.0 cps Microbiological: S.P.C. < 100 CFU/g Yeast < 10 CFU/g Mold < 100 CFU/g

3 Efficacy / Suggested Applications Moisture Control and Humectancy: TRIspire Enhance acts as an excellent moisture control additive by holding the moisture within the molecular matrix under dry condition. It has been clinically tested by an independent laboratory to measure its effectiveness for reducing TEWL and thus offers moisturization benefits to skin care formulations. As indicated in Figure 3, the lotion without TRIspire Enhance imparted statistically significant reduction in TEWL only at the measurement made 1 hour after application. In contrast, statistically significant reduction in TEWL was observed at 1, 2, and 4 hours after a single application of the lotion containing TRIspire Enhance. Clinical Testing Protocol: 22 Subjects Product applied to lower leg with a single application (2.0 mg/cm 2 ) with and without the incorporation of TRIspire Enhance 2% W/W Measurements taken at 1, 2 and 4 hours after a single application Test Formulations and Controls: Control (without GA) Test formulation with 2% GA Enhancement of AHA Exfoliation AHAs have long been known to increase skin exfoliation and have received significant use for this purpose. However, they are also well known to cause skin irritation, particularly at their effective higher concentration and at lower ph conditions. In an effort to achieve a higher level of exfoliation without accompanying increase in skin irritation, formulations containing TRIspire Enhance in combination with a relatively high concentration of AHA (maintained at a relatively low ph) were studied. In this study, topical lotions containing 8% Lactic acid at ph 2.6 (control) with 2%, 5% and 0.7% (minimal acid to adjust the ph) GA were applied on four consecutive days to the inner forearms of thirty subjects. Exfoliation was measure by visual scoring of skin cells collected on D Squame disks after a 24 hour period for four days. The percentage increase of the cumulative total D Squame scores over four days for the control and test formulations are shown in Figure 4 and 5. In Figure 4, the Day 4 reading showed that the 8% Lactic acid formulation containing 2% and 5% GA provided a cumulative increase of 16.42% and 20.46% respectively in exfoliation without irritation. This result indicates that the addition of GA to AHA formulations can achieve the higher level of exfoliation without increased potential irritation. It is important to note that GA has some primary exfoliation capability, as seen in Figure 5. Here, the test formula exhibited much greater exfoliation than the control.

4 Clinical Testing Protocol 30 Subjects Test material applied to 25 cm 2 areas of inner forearms at the concentration of 2 mg/cm 2 for 4 consecutive days Exfoliation measurements were using D Squame Surface Sampling Discs Exfoliation was measure by visual scoring of skin cells collected on D Squame disks at the end of each 24 hour period Test Formulations and Control: Control: 0% GA, 8% AHA Test 1: 2% GA, 8% Lactic Acid Test 2: 5% GA, 8% Lactic Acid Test 3: 5% GA, 0.7% Lactic Acid Skin Treatment Efficacy Evaluation A clinical study was conducted by an independent research institute to evaluate the efficacy of a test product containing galactoarabinan (GA) in improving the skin texture and in treating the skin s superficial fine lines. Fifty (50) subjects completed an eight (8) week, full face, randomized, doubleblind, placebo controlled study. In conclusion, under the conditions employed in this study, the skin erythema decreased significantly for the test formula (5% GA) compared with the control (8% Lactic Acid). The percentage reduction of erythema is 63.2% for the test formula versus the control 28.6%. Furthermore, results of replica analysis showed an improvement of 19% in superficial fine lines for the test formula. Clinical Testing Protocol: 50 Subjects between age An eight (8) week, full face, randomized, doubleblind, positive control use study Product performance was assessed visually using trained evaluators and instrumentally using the Dermaflex for measuring viscoelastic properties of skin and silicon replicas with subsequent image analysis for measuring fine line/wrinkles Test Formulations and Controls: Control: 0% GA, 8% AHA Test Formula: 5% GA, 0% Lactic Acid

5 Sunscreen Formulation SPF Enhancement The reduction of droplet/particle size in the emulsion and the improvement of pigment dispersibility and uniformity in the formulation assist galactoarabinan (GA) to exhibit SPF enhancement for sunscreen formulations. As demonstrated in the following Figure 8, the dispersion of titanium dioxide in the emulsion with GA is more even, much more fine, less whitening and without agglomerates. An in vitro instrumental study was conducted. In this study, common organic sunscreens and inorganic sunscreens with various surface treatments at moderate concentration levels were incorporated in the testing formulations. The composition of the base emulsion is shown in Table 2. The test formulas, based on this base emulsion were determined for SPF value with different sunscreen actives. SPF In Vitro Testing Protocol: SPF 290 SPF Analyzer was used for this study Vitro Skin TM was used as the substrates (by Innovative Measurement Solution) Test product was applied to Vitro Skin TM at the rate of 2μl/cm 2 SPF was determined instrumentally in duplicate runs per products with 12 reading points for each run Test Formulations and Controls: Control: 0% GA emulsion base Test Formula: Different sunscreen combinations with different levels of GA: 0%, 2% and 5%

6 From the in vitro SPF testing results (see table 3), GA displayed the ability to enhance the SPF. The magnitude of improvement ranged from as little as 5% to as much as 60%. In general, the higher the GA level, the better the enhancement. Two grades of titanium dioxide used for this study were treated with a hydrophilic coating, such as aluminium hydroxide (and) hydrated silica and aluminium hydroxide (and) hydrated silica (and) alginic acid. The hydrophobic coating titanium dioxide was aluminium hydroxide (and) stearic acid. As indicated in Table 3, GA boosts the SPF of the products containing physical sunscreens of titanium dioxide with both hydrophilic and hydrophobic coating. The results of SPF boosting effects are due to the GA acting as a dispersing aid to achieve much smaller, even and uniform distribution of particulates with minimal agglomeration. Usage areas and formulating recommendations TRIspire Enhance is extremely soluble in water and partially soluble in many solvents due to its surface activity. In any emulsion systems and surfactant systems, TRIspire Enhance should be introduced into the water phase. TRIspire Enhance does not contribute to formula viscosity, can be tolerant with a high level of electrolytes, works at a wide ph range (2 12), and sustains high shear. Packaging / Storage The product is supplied in 25kg (55lb) net weight fibre drums. TRIspire Enhance can be stored for at least four (4) years in the sealed original packaging under normal temperature conditions.

7 Regulatory Information Galactoarabinan is a food additive for direct addition to food for human consumption (US FDA ), (EINECS ). The global regulations of cosmetic applications are listed in Table 4 below. USA Country Status PC: 1996 PCPC assigned INCI; General: TSCA CAS No Australia / New Zealand General: NICNAS AICS CAS No Canada General: CEPA NDSL ( DSL Eligible) CAS No Europe General: EINECS CAS No Japan PC: JCLD approved on positive list MHW: Not restricted Food: MITI natural food additive South Korea Natural Food additive code #38 Taiwan Thailand Philippines China Food ingredient D Galacto L Arabinan Food additive Ministerial Notification PICCS 2007 List of food additives (Bureau of Circular No , Oct. 18, 2006 Galactoarabinan listed on Third Batch of IECIC Draft 2012 Toxicological Information Refer to MSDS Dermal Irritation: There have been no known irritation effects RIPT testing on 58 individuals showed no evidence of irritation or sensitization Neytral Red Uptake Bioassay found TRIspire Enhance to be very mild to human epidermal keratinocytes (skin cell) Ocular Irritation: Mattel Corporation EPI Ocular in vitro skin mode; classified TRIspire Enhance as a non irritant. Mutagenicity: Ames Test showed not mutagenic+

8 Ecological and Ecotoxicological Information Refer to MSDS Safety Precautions Please see the Material Safety Data Sheet before handling the material. For further information, please contact your local TRI K representative. Contact TRI K Industries via at info@tri k.com, visit the TRI K website at k.com, or you can reach TRI K Industries at +1 (973) Warranty. This information herein is offered as a guide and is believed to be accurate and reliable as of the date of the printing. The values given are not to be considered as a warranty and they are subject to change without prior notice. For additional information regarding our products or for information concerning current specifications, please contact our Technical Service. 01/2013

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