SERISEAL DS. Positively reverses hair damage

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1 SERISEAL DS Positively reverses hair damage

2 Seriseal DS Positively reverses hair damage Substantivity by adhesive effect Adds proteins Restores and protects hair fiber Restores hair to its natural state

3 Seriseal DS Protein derived from the silkworm Bombyx mori Aminoacids content: 33% Serine 20% Aspartic Acid 14% Glycine 8% Threonine Properties: strong similarity to other proteins able to bond to hair keratin

4 Silkworm Sericin Sericin Solution

5 Seriseal DS Positively reverses hair damage Guar Gum Sericin Solution Sericin Solution CCTA + Behentrimonium Chloride Seriseal DS

6 SEM - Scanning Electron Microscopy Chemically damaged hair Damaged hair Before treatment Damaged hair After treatment with Seriseal DS * * 5% Seriseal DS with water

7 Glossmeter - Increase in Shine Dark brown caucasian hair damaged by chemical bleaching treatment 1% Seriseal DS Placebo* 60.4% 181% 3% Seriseal DS Placebo* 102% 237.1% 5% Seriseal DS Placebo* 140.5% 330.4% *Placebo: Same components of Seriseal DS, exempt the interlaced protein.

8 Glossmeter - Increase in Shine Dark brown caucasian hair damaged by chemical bleaching treatment 5% Keratin 33% 5% Seriseal DS 80% * Aqueous solution

9 Volume Reduction (cm) Dark brown caucasian hair with regular curl damaged by chemical bleaching treatment 1% Placebo* 3% Placebo* 5% Placebo* 1% Seriseal DS 3% Seriseal DS 5% Seriseal DS *Placebo: Same components of Seriseal DS, exempt the interlaced protein.

10 Volume Reduction (cm) Dark brown caucasian hair damaged by chemical bleaching treatment Water Water + Seriseal DS Tresses 13cm treated with water Tresses 7cm treated with Seriseal DS 5%

11 Redensification Cross Sectional Area (µ 2 ) 4673, ,04 Seriseal DS Increased redensification by 9.34% Control * 5% Seriseal DS * * Aqueous solution

12 Strength Maximum Strength (Gfm) 79,30 90,10 Seriseal DS Increased strength by 13.62% Control * 5% Seriseal DS * * Aqueous solution

13 Sensorial Efficacy Assessment of hair strands through sensory analysis Caucasian damaged strands Application - Seriseal DS (w/w) in water - Placebo - Water Evaluation panel Ten trained evaluators scored Evaluated parameters - Damage reduction - Softness - Combing

14 Damage Reduction Number of evaluations Not satisfactory Placebo Seriseal DS Very satisfactory Little satisfactory Satisfactory Indifferent

15 Softness Number of evaluations Not satisfactory Placebo Seriseal DS Very satisfactory Little satisfactory Indifferent Satisfactory

16 Combing Number of evaluations Not Satisfactory Placebo Seriseal DS Very Satisfactory Little satisfactory Indifferent Satisfactory

17 SERISEAL DS Hair Dyes

18 Treatment of Tresses Dark brown caucasian hair Chemical treatment bleaching Dyed with 6.64 tone Treatment Placebo 3% Seriseal DS (BYK Gardner) Color Spectrophotometer Spectro-Guide Sphere

19 Formula Evaluated

20 Formula Evaluated

21 Instrumental Evaluation Colorimetry First Washing Tone 6.64 ΔE Placebo = 7,20 ΔE Seriseal DS = 4,11 Color Maintenance 42.9%

22 Instrumental Evaluation Colorimetry Fifteenth Washing Tone 6.64 ΔE Placebo = 6,94 ΔE Seriseal DS = 4,36 Color Maintenance 37.2%

23 Results Color intensity, better than placebo formulation Intensifies up to one (1) tone, and remains superior even after 15 washings when compared to the placebo formulation Improves the shine and texture of hair tresses (Sensorial analysis)

24 SERISEAL DS Hair Straightening

25 Study Protocol 1-2% of Seriseal DS with: Guanidine Carbonate Ammonium Thioglycolate Monoethanolamin Thioglycolate This study is compared with placebo and benchmark Objectives: Evaluate Seriseal DS contribution during the process of straightening Improve texture and appearance reduce damage.

26 Formula Evaluated - Placebo

27 Formula Evaluated 1% Seriseal DS

28 Formula Evaluated 2% Seriseal DS

29 Guanidine Carbonate Benchmark Placebo Seriseal DS 1% Seriseal DS 2%

30 Formula Evaluated Placebo

31 Formula Evaluated 1% Seriseal DS

32 Formula Evaluated 2% Seriseal DS

33 Ammonium Thioglycolate Benchmark Placebo Seriseal DS 1% Seriseal DS 2%

34 Formula Evaluated Placebo

35 Formula Evaluated 1% Seriseal DS

36 Formula Evaluated 2% Seriseal DS

37 MEA Thioglycolate Placebo Seriseal DS 1% Seriseal DS 2%

38 Results The hair fiber shows better alignment and straighter appearance Frizz and volume reduction Better product absorption and compatibility Improved spreadability easier product application

39 Seriseal DS Molecular shine Immediate, visible results Repairs damaged hair Works with the hair s natural structure Stronger adhesive effect

40 Seriseal DS Application Hair conditioners Treatment masks Leave on products Suggested concentration 1 to 5 % (p/p) INCI Water (and) Polyquaternium-7 (and) Sericin (and) Guar Hydroxypropyltrimonium Chloride (and) Cetrimonium Chloride (and) Behentrimonium Chloride

41 Seriseal DS Appearance: slight viscous to viscous liquid Color: white yellowish to beige ph (as such): 5,50 7,50 Average protein content (% w/w): 2,5 Average nanoparticles size: 150nm Maximum quaternary concentration: 1.6% Maximum concentration of polyquaternium 7 am: 2.4%

42 THANK YOU!

43

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