TRICHOGEN VEG LS 8960

Transcription

1 TRICHOGEN VEG LS 8960 Trichogenic complex Anti hair loss active and conditioner / 1/35

2 Physiology of hair Stratum corneum Epidermis Basal layer Dermis Zone of permanent hair Sebaceous gland Arrector muscle Keratogenous zone Germinative bulb Area of differentiation and biological synthesis / 2/35

3 Hair stem Protofibril Microfibril Macrofibril Exocuticl Endocuticle e Cell membrane complex Cortex Cuticule 70 A 50µ 0.7µ / 3/35

4 Hair follicle Bulb Papilla The hair follicle is the living part of the hair: - the bulb Made of cells, similar to basal epidermal layers Metabolic activity is important - the papilla Median invagination High vascularization / 4/35

5 Physiology of hair follicle HAIR CYCLE Hair follicles have a periodic and cyclic activity of growth Anagen phase 3 years Catagen Telogen phase phase 3 weeks 3 months / 5/35

6 Cyclic activity can be evaluated by a trichogram Anagen Phase (aspect of the bulb) Telogen Phase (aspect of the bulb) / 6/35

7 Anagen phase OES ES p IE S The anagen phase is an active phase of growth, A p B 75 µm 50 µm = keratogenous area ES = epithelial sheath IES = inner epithelial sheath OES = outer epithelial sheath g = germinative cells p = dermal papilla it lasts 3-4 years / 7/35

8 The hair formula The trichogram determines: the Hair Formula 15% 1% Anagen hair Telogen hair Catagen hair A T The A ratio T = Anagen hair % Telogen hair % A T = 4 : normal situation 84% A T = < 3 : Pathological ratio / 8/35

9 Androgenic or seborrheic alopecia on man I II III III Vertex Classification according to Hamilton IV V VI VII / 9/35

10 Androgenic or seborrheic alopecia on woman Ludwig classification (on women) Androgenic alopecia is partly linked to the action of androgens on the papilla / 10/35

11 Origin of androgenic alopecia Inner factors - receptivity to male hormones - genetic tendency - psychic factors Nutritional factors - deficiencies in proteins sulfur amino acids in vitamins - general deficiencies Seborrhea / 11/35

12 Definition TRICHOGEN VEG LS 8960 is an unique trichogenic complex. It consists of a selection of synergistic components of botanical, biotechnological and synthetic origin. Profollica Treatment for men / 12/35

13 Composition and mode of action Sulfopeptides of soy (glycine max) (MW ~ 15,000 Da) Amino-acids: tyrosine, arginine, ornithine, citrulline Aminosaccharide glucosamine Regulators of cellular nutrition and growth. Supply in bioavailable sulfur, stimulation of protein biosynthesis Precursors of proteins and melanin pigments GAG precursor for strengthening the extracellular papillary / peribulbar matrix / 13/35

14 Composition and mode of action Vitamins of B group (PP, B5, biotin) Zinc salt Regulation of cellular nutrition Seboregulation Stimulation of microcirculation Antimicrobial effects Panax Ginseng extract Arctium Majus extract Silicone and quaternium Hair conditioner / 14/35

15 Efficacy test (clinical test) Anti-hair loss activity 40 male volunteers with androgenetic alopecia (I to III) A/T ratio < 4 D0: sensorial and clinical appraisal - Trichogram Treatment every two days 10 ml of hair lotion containing 10% TRICHOGEN VEG LS 8960 D56 - D112 Sensorial and clinical appraisal by the volunteers and a dermatologist Trichogram - Phototrichogram / 15/35

16 Efficacy test (clinical test) Evaluation by the volunteers Improvement of aesthetic qualities of hair and scalp Sensorial evaluation Parameters Shape, volume Spring Shine Silk Seborrhea Irritation Improvement (% subjects) after: 56 days 112 days Good Quite good Good Quite good / 16/35

17 Efficacy test (clinical test) Evaluation by the volunteers Decrease of hair loss Semi-quantitative appraisal by volunteers of hair loss % (NS) -24% ( ) -37% (***) -41% (***) -38% (**) -45% (**) Combing Shampoo On the pillow D0 D56 D112 Statistics: Student's t test (***) p (**) p 0.01 (*) p 0.05 ( ) p 0.10 (NS) = not significant / 17/35

18 Efficacy test (clinical test) Evaluation by the volunteers Improvement of hair growth Lower hair loss Faster hair growth 10% 10% 7.5% 12.5% 5% 40% 35% 47.5% 40% 12.5% D % 30% 60% 17.5% D56 D112 50% D112 No effect Medium Good Quite good / 18/35

19 Efficacy test (clinical test) Evaluation by the volunteers The volunteers have observed: - an improvement of the aesthetic qualities of hair and scalp, - a lower hair loss, - a faster hair growth - source : / 19/35

20 Efficacy test (clinical test) Evaluation by a dermatologist Improvement of the aesthetic qualities of hair and scalp Dull hair of satisfied subjects 0% 50% 100% Hair being hard to comb Lack of suppleness Thin hair D56 Fly away effect 50 Oily hair D112 Splitted hair Volume Irritated scalp / 20/35

21 Efficacy test (clinical test) Trichogram evaluation Visualization Telogen hair Anagen hair (X 40) / 21/35

22 Efficacy test (clinical test) Trichogram evaluation Quantification Anagen hair / Telogen hair ratio (A/T) % (***) + 138% (***) + 26% 9.8 Alopecia threshold (NS) + 106% ( ) % (***) + 53% (*) % (***) + 113% (***) Frontal Parietal Occipital 3 areas total D0 D56 D112 Statistics: Student's t test (***) p (**) p 0.01 (*) p 0.05 ( ) p 0.10 (NS) = not sign / 22/35

23 Efficacy test (clinical test) Phototrichogram evaluation Before treatment After treatment 1 mm 1 mm Same area after 4 months of treatment: new hairs appear compared to before treatment, each one in anagen phase / 23/35

24 Efficacy test (clinical test) Anti-hair loss activity After 4 months of treatment, TRICHOGEN VEG LS 8690 clearly : Slows down hair loss. Improves hair regrowth : the growth is more important, and faster, on the parietal, occipital and frontal zones. Improves the aesthetic quality of hair and scalp. These effects are quantified, and visible on pictures and by the users. :Buy Profollica Now!!! / 24/35

25 Other tests available Strengthening effect on damaged human hair (traction test) Film-forming, bio-conditioning and repairing effect on dry hair (confocal microscopy) Conditioning effect Photo-protection of human hair, against UV-B induced damage (dansyl chloride) Stimulation of oxygen consumption (epithelial cells) / 25/35

26 Efficacy tests Strengthening effect on damaged human hair (Traction test) Protocol Once or 5 times Hair - Sodium Lauryl Sulfate at 15% Permanent wave Mercaptoacetate at 6% + H 2 O 2 at 2% + rinsing 2 cycles of permanent have been done Without treatment Placebo lotion Lotion with 5% TRICHOGEN LS ml of lotion on hair tress during 30 min + rinsing 24 h after the last treatment Traction test: after 1 treatment and after 5 successive treatments / 26/35

27 Efficacy tests Strengthening effect on damaged human hair (Traction test) F3 F1 = elasticity limit F2 = plasticity limit F3 =break point Work to break F15 =force at 15% of elongation Post-yield slope % of increase F15 Parameters After 1 treatment After 5 treatments Load F1 F2 Force at 15% + 9% + 6% Yield region Post-yield region Maximal force + 10% + 23% Post-yield slope + 11% + 62% Hookean region Strain The strengthening activity of TRICHOGEN VEG LS 8960 at 5% on damaged hair is clearly demonstrated: the increase of hair mechanical parameters is significant even after only one application / 27/35

28 Efficacy tests Film-forming, bio-conditioning and repairing effect on dry hair Confocal microscopy Hair was damaged by an acetone/ether blend and then treated with TRICHOGEN VEG LS 8960 at 10% Control hair Damaged hair with acetone/ether Damaged hair treated with TRICHOGEN VEG LS 8960 TRICHOGEN VEG LS 8960 at 10% has clearly improved the surface of hair, and shows a good hair conditioning / repairing effect / 28/35

29 Efficacy tests Conditioning effect Quantitative evaluation of the conditioning effect of a lotion containing 10% TRICHOGEN VEG LS 8960 Traction device cell Traction device recorder Mean value of the combing parameters ± SEM % (*) % (*) Before treatment After treatment 20 Comb Statistics: Average of 10 trials ± SEM paired t test (*) significant/before treatment Hair tress 0 Work (Nmm) 0 Maximal force (N) TRICHOGEN VEG LS 8960 at 10% has significantly improved combing parameters of hair. It has a good conditioning property / 29/35

30 Efficacy tests Photo-protection of human hair, against UV-B (dansyl chloride) Results Untreated control hair Hair lock Standard washing with Sodium Lauryl Sulfate (= SLS) at 5% Treatment with a hair lotion containing 10% of TRICHOGEN VEG LS 8960 Fluorescence intensity (Arbitrary units) No UV-B With UV-B* % (*) Protection * UV-B 270 J/cm No UV UV UV Statistics: Mann Whitney's U test (*) significant/corresponding control Reaction with dansyl chloride Fluorescence quantification 0 Untreated control 0 Untreated Hair lotion control with 10% of TRICHOGEN VEG LS 8960 TRICHOGEN VEG LS 8960 has shown a strong photo-protective effect of hair (increasing the fluorescence intensity by +28.8%) / 30/35

31 Efficacy tests Stimulation of oxygen consumption Polarographical measurements on homogenate of epithelial cells % increase of oxygen consumption % + 51% (*) + 67% (*) + 74% (*) Concentration of TRICHOGEN VEG LS 8960 (%) Statistics: Average of 5 trials ± SEM Kruskal Wallis's test: Significant A posteriori test: Mann Whitney U test (*) significant difference in comparison to 0.5 % Significant and dose-dependent increase of oxygen consumption by epithelial cells. TRICHOGEN VEG LS 8960 clearly improves cell vitality / 31/35

32 Cosmetodynamic properties Decrease of hair loss and stimulation of hair growth cellular metabolism and oxygenation are increased Improvement of the aesthetic qualities of hair improvement of hair softness, vitality, combability, suppleness, health Improvement scalp condition the scalp is healthier sebaceous secretion is regulated dandruff, itchings are decreased / 32/35

33 Cosmetic uses Anti-hair loss preparations Anti-age hair care Formulations leave-on preparations: aqueous, hydro-alcoholic tonics rinse-off preparations: shampoos, conditioners / 33/35

34 Technical data Aspect : limpid light yellow liquid with a characteristic odor Dose of use : Anti-hair loss courses: 10% As complementary hair care: shampoos: 3-5% Solubility INCI Name : soluble in water, in hydroalcoholic solutes and insoluble in oils : Water (and) Panax Ginseng Root Extract (and) Arginine (and) Acetyl Tyrosine (and) Arctium Majus Root Extract (and) Hydrolyzed Soy Protein (and) Polyquaternium-11 (and) Dimethicone Copolyol (and) Calcium Pantothenate (and) Zinc Gluconate (and) Niacinamide (and) Ornithine HCl (and) Citrulline (and) Glucosamine HCl (and) Biotin / 34/35

35 TRICHOGEN VEG LS 8960 The effective active ingredient against hair loss / 35/35