Kewpie. Corporation. Fine Chemical Division. Kewpie strives to provide attractive products to please our customers.

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1 Kewpie Corporation Fine Chemical Division Kewpie strives to provide attractive products to please our customers.

2 Contents Product list 1-4 Sodium Hyaluronate 5-6 Hyalo-Oligo 7-8 Hyaloveil -P 9-1 Hyalorepair Natural ingredients Nutriceutical 15 Chronology of new product launches 16

3 Products of Kewpie Fine Chemical Division Ecocert Chicken Corn Fermentation Seed Chicken egg Chicken Comb Egg Yolk Egg White Eggshell Membrane Hyaluronic acid (Sodium hyaluronate) Egg Yolk Lecithin Lysozyme EM Protein Molecular weight range Dose Expiry(condition) Packing (Million Da) Egg Yolk Lysolecithin Egg White Peptides EM Powder Egg Yolk Oil Albumin Phytoglycogen HA-M57 HA-Q months temperature) 1g 1 Cosmesome series Hyaluronic Acid (Sodium Hyaluronate) Strict manufacturing management system guarantees high quality ISO 91: 28 (JIS Q91: 28) ISO 141: 24 (JIS Q141: 24) HA-LQ HA-LQH HA-LQSH Sodium Hyaluronate We also have 1% solution of above marked products. Please contact us for availability. 24 months temperature) 12 months temperature) 12 months (1 ~1 ) 1g 1 Functional hyaluronic acid Molecular weight range Dose Expiry(condition) Packing Remark (Million Da) Hyalo-Oligo Hydrolyzed Hyaluronic Acid Less than Free acid Hyaloveil -P Hydroxypropyltrimonium Hyaluronate.5-.8 (Reference) months temperature) 1g 1 Cationized Goka Factory, Fine Chemical Division Hyalorepair C12-13 Alkyl Glyceryl Hydrolyzed Hyaluronate Less than Alkylated We also have 1% solution of above marked product. Please contact us for availability. 1 2

4 Eggshell membrane products Eggshell membrane is a thin film inside the eggshell The following products are derived from egg protein. Cosmesome Cosmesome is the pre-mixed preparation of egg yolk lecithin and various cosmetic raw materials adopted our original technique and can be used for wide range of cosmetics. Composition Characteristics Dose Expiry Packing Function (condition) EM Protein-P EM Protein-L EM Powder (3) Hydrolyzed Egg Shell Membrane Hydrolyzed Egg Shell Membrane Butylene Glycol Methylparaben Water Egg Shell Membrane Powder Water soluble powder being hydrolyzed 1% solution of EM Protein-P Powder made by drying and crushing eggshell membrane months temperature) 24 months (1 ~ 1 Freezing is strictly prohibited.) 24 months temperature) 1g 1 5kg 2 Improve dry and rough skin. Promote production of Type-III Collagen Emollient effect Composition Characteristics Dose Expiry Packing Function (condition) Cosmesome CM-1 Cosmesome L-1 Water Glycosphingolipids Phenoxyethanol Hydrogenated Lecithin Lysolecithin Sodium Hydroxide Citric Acid Lysolecithin Glycerin Dipropylene Glycol Less than 1.% The preparation in which plant ceramide is dispersed without using any of synthetic surfactants nor organic solvents Excellent lysolecithin preparation as emulsifier, dispersant and solubilizer months temperature, Freezing is strictly prohibited) 12 months temperature, Flammable) Moisturizer, emollient, improver for rough and dry skin Moisturizer, emulsifier, dispersant Egg yolk products Functional lipid called "Phospholipid" which contains phosphorus in the structure. Phospholipid plays an important role in sebum/hair and can be used for wide range of cosmetics. Phytoglycogen Plant-derived glycogen extracted and refined from sweet corn seed This Phytoglycogen has a smooth feeling without stickiness and can be used for wide range of cosmetics. Composition Dose Expiry Characteristics Packing Function (condition) Egg Yolk Lecithin PL-3S Egg Yolk Lecithin PL-1P Egg Yolk Lysolecithin LPC-1 Egg Oil Hydrogenated Lecithin Lysolecithin High viscous oil containing more than 3% of phospholipid Purified egg yolk lecithin powder that is stabilized by hydrogenation. Powder mainly consisted of lysophosphatidylcholine that is stable even in solbilized transparent phase months (Under 1 ) 36 months (Under 1 ) 12 months (Under 1 ) 1kg 6 1g 1 3kg 1 Moisturizer, dispersant, solubilizer, emolient Composition Dose Expiry Characteristics Packing Function (condition) Phytoglycogen Glycogen 1 Plant-derived glycogen powder extracted from sweet corn months temperature) Moisturizer Egg white products Egg white has water retaining function, gives skin elasticity, gloss and protects hair as well. Lysozyme Hydrochloride has anti-inflammatory effects (mainly for pharmaceutical API). It can be used for wide range of cosmetics. The following products are derived from egg protein. Composition Characteristics Dose Expiry Packing Function (condition) Albumin Egg White Peptides EP-1 Albumen Hydrolyzed Albumen 1 1 Spray-dried powder that is the desaccharized egg white Powder enzymatically hydrolyzed egg white with high heat stability months temperature) 5kg 2 Moisturizer Lysozyme Hydrochloride Lysozyme 1 Enzyme extracted from egg white, having decomposing function of mucopolysaccharides months temperature) 1g 1 Anti-inflammatory effects 3 4

5 ) This gives moisture to the skin and hair due to its excellent water holding / moisture retention properties. List of the patents of functional hyaluronic acid Kewpie is the only manufacturer who produces hyaluronic acid by both extraction from chicken comb and bio-fermentation in Japan. We have our own special technique to control the molecular weight of hyaluronic acid suited to the customers' need. PAT. JP B2, EP18652 B1 PAT.P. WO26/113 A1 PAT. JP B, CN B PAT.P. WO28/ A1 PAT. JP B2 PAT.P. WO211/12462 A1 1. High moisture retention Kewpie's hyaluronic acids meet various demands and keep progress. The increasing ratio of skin moisture content( %)conc. of HA-LQH Applying.5,.1,.3 and.5% of the solutions of HA-LQH onto the subject's forearm and measured the skin moisture content after 5 minutes. Measuring equipment: SKICON-2 Increase amount of moisture in the skin ( μs The group applied.1 % solution of HA-LQ The group applied water P <.5 (Compare with the condition before applying sample) Before application Time (Minutes) Applying.1% solution of HA-LQ onto the subject's forearm and measured the skin moisture content over time. (after 5 minutes, 1 minutes and 2 minutes) Measuring equipment: SKICON-2 Functional hyaluronic acid NO Are you satisfied with the hyaluronic acid currently using? YES To increase penetration of hyaluronic acid To increase adhesiveness of hyaluronic acid To approach barrier function of the skin.5 Million ~.7 Million Da.53 Million ~ 1.33 Million Da HA-M57 HA-Q (Chicken comb) (Chicken comb) It was confirmed that the solution of HA-LQH made the skin moisture content higher in proportion to its concentration. HA-LQH is a granulated powder Easier to be dissolved even with low power stirrer. Easier to handle (Hard to airborne) It was confirmed that the solution of HA-LQ increased the skin moisture content over time significantly and such high content was kept even 2 minutes after the application. 2. Solubility into water 3. Transparency of HA-LQH solution We have confirmed high transparency (absorbance) of cosmetic grade HA-LQH as follows: Absorbance(1 Absorbance of 1% solution Sodium Hyaluronate What is the molecular weight of the hyaluronic acid currently using?.85 Million ~ 1.6 Million Da 1.2 Million ~ 2.2 Million Da 1.6 Million ~ 2.9 Million Da Variety of hyaluronic acids and their functions HA-LQ HA-LQH HA-LQSH (Fermentation) (Fermentation) (Fermentation) Adsorptive type hyaluronic acid "Adsorb and last" Hyaluronic acid covering the surface of the skin "Retain Moisture" Sebum When charged 5 minutes later % )8 4nm 66nm (wavelength) (Yellow) (Turbidity) Absorbance of 1% aqueous solution of HA-LQH was measured at 4nm and 66nm. In each case absorbance in nearly 1%, which proves very high transparency of HA-LQH solution. Hyaluronic acid reparing barrier function (Figure) Horny layer Granular layer Epidermis Hyaluronic acid penetrating into the skin"keep the Moisture Inside" 5 6

6 ) PENETRATION type hyaluronic acid Hyaluronic acid with the average molecular weight of lower than 1, Da. The nano-size molecule making itself penetrate into horny layer PAT. JP B2, EP18652 B1 PAT.P. WO26/113 A1 Because Hyalo-Oligo covers the skin surface and also penetrates into horny layer, it keeps the skin moisture both inner and outer side of the skin. Hyalo-Oligo which remains in horny layer of the skin is not washed away, thus unlike common hyaluronic acid, it retains the skin moisture even after washing. 1. Transdermal absorption 3. Effectiveness on the wrinkles Grade Grade1 Grade2 Grade3 Grade4 Grade5 Grade6 Grade7 Journal of Japanese Cosmetic Science Society Vol. 3, No. 4 Decreased volume of wrinkles Start 4th week 8th week Hyalo-Oligo 1% solution Water Decreased volume of wrinkle = (The grade on the test date) - (the grade on the start date) Wrinkles Decrease Horny layer Epidermis Applied 1% solution of twice a day (morning & night) for 8 weeks. Visual confirmation by the doctor to see wrinkle degree change (before/ after). Consigned testing laboratory: Inforward Inc. Ebisu skin reasearch center The test result confirmed that Hyalo-OligoR on wrinkle appearance. is effective Dermis Hematoxylin/eogin dying of skin Consigned testing laboratory: BIO alternatives Unique fluorescence 4. Deep penetration into hair The result of the test with human skin confirmed that fluorescent coloring is seen in the horney layer of skin where Hyalo-Oligo has penetrated in. It was confirmed that H yalo-oligo has penetrated into the horney layer of skin. 2. High moisture retentivity Increase amount of moisture in the skin ( μs Before application Days after application Common hyaluronic acid Damaged hair (Control) Bleached and damaged hair was soaked in 1% solution of with fluorescence label at 4 for 15 minutes, washed, dried and observe this hair section. Damaged hair treated with The shining degree shows the penetration of It is confirmed that Hyalo-Oligo penetrated into the hair inside. The gauze soaked in 1% solution of was attached to the human forearm for 24 hours. Measuring the skin moisture amount after the gauze was detached. Measuring equipment: SKICON-2 It was confirmed that Hyalo-Oligo increased twice as much the skin moisture as common hyaluronic acid. (Presented at the Pharmaceutical society of Japan, March 26) 7 8

7 ADHESION type hyaluronic acid Not easily washing off from skin and hair due to partial cationization. PAT. JP B, CN B PAT.P. WO28/ A1 3. Adhesion test of Hyaloveil -P to the hair In addition to "retaining moisture" and "visco-elsticity" of conventional hyaluronic acid, "adhesive" feature of Hyaloveil-P makes it possible to stay longer on the hair and the skin and not easily washes off. "Moisture Veil" of " H yaloveil-" covers the surface of the skin and the hair to keep it moist even after cleansing or shampoo. Volume adhered to 1gram of hair 1. Adhesion to the skin Common hyaluronic acid Soaking the damaged hair (bleached hair) into.5% solution at 4 for 1 min. Rinsing with water and drying. (Analyzed by HPLC) Adhesion to the skin Common hyaluronic acid Applying the 1% solution of to the subject s forearm. Leaving it at rest for 3 minutes. Washing the sample off the forearm by running water. Sticking and stripping adhesive tape. Measuring the residue of hyaluronic acid in the adhesive tape by HPLC analysis. HyaloveilR-P was not washed off but remained more on the skin attributed by its ionic bonding, compared with common Hyaluronic Acid. HyaloveilR-P was not washed off but remained more on the hair attributed by its ionic bonding, compared with common hyaluronic acid. 4. Repair the lift-ups of cuticles of the hair Electron Micrograph 2. Skin moisture retention test after washing face with cleanser Increase amount of moisture in the skin ( μs ) minutes later 1 minutes later Cleanser without any Hyaluronic Acid (Control) Cleanser with.1% Cleanser with.1% common Hyaluronic Acid Washing face with the cleanser. Washing the cleanser off by running water. Removing water from the surface of the skin. Measuring moisture contents by time (5 min later 1 min later) using skin surface stratum corneum moisture meter(skicon-2) It was confirmed that skin moisture after washing was kept and retained by formulating Hyaloveil-P into facial cleanser. Untreated Hair (Control) Soaking the damaged hair (bleached hair) into 1% solution at 4 for 1 min. Rinsing with water and drying. Observation with Scanning Electron Microscope (SEM). The lift-ups of cuticles of the hair was repaired on the treated hair with Treated Hair with 9 1

8 Hyaluronic acid featuring Barrier Function Recovery Brand new hyaluronic acid featuring barrier function recovery in the skin PAT. JP B2 PAT.P. WO211/12462 A1 is chemically modified its hydrophobic group by introduction through glycerin backbone. In addition to "Moisture retention" function, it also has new "barrier function recovery" one. 1. in vitro test / Confirmation of lamella structure formation With water With 3. Recovery of the barrier function Variation (2 TEWL (3 days later) P< %)4 Water 1%solution of 1% solution of sodium hyaluronate Improvement Measurement of transepidermal water loss(tewl) ( ) Hyalorepair prevents skin moisture from evaporation (Presented at the Pharmaceutical society of Japan, March 212.) 4. Improvement of damaged skin Number of Maltese Cross = 1 (Average) Number of Maltese Cross = 1 (Average) Replicated intercellular condition of dry skin Added Microscope photographing Lamella structure being confirmed (Circled in red) 2. Approach to Barrier function recovery mechanism (including hypothesis) After damage processing Improvement 5 days later (applying everyday) Observation by microscope photographing ( ) It was confirmed that improved the moisturization of the skin. (Presented at the Pharmaceutical society of Japan, March 212.) Healthy skin Dry skin Repaired skin (formerly dry skin) Horny cell Healthy skin: Lamellar layer acts as barrier to prevent skin from water evaporation. Dry skin: Out-balanced lamellar layer causes disorder of barrier function, resulting in dry skin by water evaporation. Damage the forearm skin with mixed solution of acetone and ether. Apply the 1% solution of everyday to the damaged skin before bedtime ( )Testing method of 3 and 4 are the same. Lipid layer Lamella layer Water layer Lipid layer Water layer Lipid layer "Wedge action" to joint water layer and lipid layer Repaired skin: Hyalorepair repairs out-balanced lamellar layer. When it s repaired, the barrier function gets recovered, which helps skin to retain moisture. Horny cell 11 12

9 Eggshell membrane Egg yolk phospholipid Eggshell membrane is a thin inside skin of eggshell and has been used to cure wound for centuries. It already appeared in Honzou-koumoku,a corpus of traditional Chinese medicine compiled in 16th century. EM Protein is a hydrolyzed eggshell membrane, which is made from eggshell membrane and made as soluble inwater by our original production method. Patented : Emulsifying Dissolving Smooth feeling 1. Promotes production of Type-III Collagen Natural sourced Biocompatibility Moisture retention The structure of Lysolecithin is not the same as lecithin, The characteristic of emulsification is also different from the lecithin. Lecithin Lysolecithin Papillary layer <Egg Yolk lecithin PL-1P> Hydrogenated egg yolk lecithin <Egg Yolk Lysolecithin LPC-1> Lysophosphatidylcholine Epidermis - Emulsifier, Emollient agent - Solubilizer Untreated Coated with EM Protein (.1μg/cm2) Type-III Collagen Incubating human dermal fibroblast in Petri-dish for two days Type-I Collagen Dermis Subcutaneous tissue 2. Increases skin elasticity Fibroblast (sectional model of skin) Type-III Collagen exists mainly in papillary layer of dermis, particularly in large volume in fetus s and baby s skin. It decreases by aging, suggesting that it plays an important roll to keep baby s skin plump and supple. Phytoglycogen Elasticity (R2) variation Start Elasticity (R2) variation : an average of 4 test subjects days 14days 21days 28days Treated Untreated Recover fibroblast function Absorbance (545nm) 1μg/cm² 1μg/cm².1μg/cm² Phytoglycogen Untreated Storage period (Day) On the suspension cultured multiplate, prepare the culture bases of Phytoglycogen to be.1, 1 and 1 μg/cm2 to culture fibroblast. How to count the number of cells Dying fibroblast with crystal violet method and measure absorbance at 545nm. It was confirmed that Phytoglycogen tended to adhere to human cells and grew human skin fibroblasts. In other words, it has high biocompatibility. Damage the forearm skin of 2 subjects with 9% SDS. Apply 1% solution of EM Protein-P everyday for 28 days to these 2 subjects and other 2 subjects who has dry skin. It was confirmed that damaged skin and dry skin were both improved

10 Nutriceutical As you get aged, hyaluronic acid in your skin decreases. The egg, from which a chick is hatched, contains all the components needed to create new life. Kewpie Corporation uses, handles, and sells approximately 2, tons of eggs annually, and for over 2 years, we have extracted and refined biologically active egg components for use in food, cosmetics, and pharmaceuticals We have applied special techniques to develop methods for extracting and refining these components from a variety of natural resources. And our products have been used not only in Japan, but also in countries across the world. Volume of Hyaluronic Acid in the skin The Decrease of Hyaluronic Acid by Aging Age (year) 8% Lost Chronology of new product launches Y e a r Egg Yolk lecithin Lysozyme Hydrochloride As you get aged, hyaluronic acid contained in your skin decreases. Then, the skin starts to lose its firmness and velvety touch and you feel aging of your skin. Maria O. Longas, et al, Carbo hydr Res 1987;159: The improvement of dryness of the skin Hyaluronic acid for cosmetics use (Extracted from chicken comb ) Egg White peptide (Hydrolyzed Egg White) Egg Yolk Lysolecithin Hydrolyzed eggshell membrane Hyaluronic acid for food use (Extracted from chicken comb ) Incresed amount of skin moisture (a.u) Before intake Hyaluronic acid 12mg/day Placebo weeks 6 weeks 2week after intake ends <Beauty from In & On> Moisture retention by hyaluronic acid Hyaluronic acid for pharmaceutical use (Extracted from chicken comb ) Icosapentosan EQ (EPA Ethyl Ester) for pharmaceutical use Condroitin Sulfate House-brand supplement series Hyaluronic acid made by fermentation method for cosmetics and food use Hyalo-Oligo Test food: HA-LF-P supplement (12mg/day) Placebo: Crystalline cellulose Test subjects: Japanese female from 35 age to 6 age, 22 persons/group s: Double-blind test (parallel 2groups) Measured item: Measurements of cheek skin moisture Measured device: CORNEOMETER CM825 It was confirmed that oral intake of Hyarulonsan HA-LF-P improved dryness of the skin. Oral intake Nutriceutical Improves dryness of the skin External application Cosmetics Relieves the problem of skin and hair Hyaloveil -P,Hyabest (J) Hyalorepair 15 16

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