Total hair growth solution

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1 Total hair growth solution Your first experience. Your last chance. Decapeptide-18 Octapeptide-2 Oligopeptide-54 Octapeptide-11 Decapeptide-1 Day.3 Day.6 Day.9 Day.12 Day.15 Oligopeptide-71 Decapeptide-28

2 DR CYJ HAIR FILLER - The 1st Hair Filler in the World. Developed by Innovative peptide complex for Hair re-growth, Scalp re-construction. Decapeptide-18 Octapeptide-2 Oligopeptide-54 Decapeptide-1 Octapeptide-11 Day.3 Day.6 Day.9 Day.12 Day.15 Oligopeptide-71 Decapeptide-28 DAY_3 DAY_6 DAY_9 DAY_12 DAY_15 DR.CYJ Hair Filler (1 ml) DR.CYJ Hair Filler Cocktail (1.5 ml) Application Non-Scaring Alopecia Patients. Man & Woman who have a general thinning hair. Hair transplant recipients who want to increase survival rate. Alopecia patients who want to have Synergic effect combining with other application for anti-hair loss / hair re-growth. Advantage 1. Patented and exclusive peptide complex for hair regrowth and health. 2. Sustained Release technology to maximize the efficacy of 7 hair growth peptides. 3. Applicable and effective to any type of non-scaring Alopecia. 4. Clinically proven. Direction for use Protocol 1) Apply topical anesthetic cream on the target scalp area 2) After 3min, clean the applied cream then sterilize with alcohol swab 3) Inject DR.CYJ Hair Filler on the target area with 3G Needle! Injection Technique: Point Injection " How to inject! Injection point every.2~.3 cm! 1.cm per line! Inject.2~.5 ml per point Standard Treatment : 1 session every 2 weeks for 8 weeks (Total: 4 Session) Intensive Treatment : 1 session every 2 weeks for 8 weeks (Total: 4 Session) + 1 session every month for 3 month (up to 6 month) Post professional therapy: Apply Pelo Baum Hair revitalizing solution every morning and night on a daily basis. 2

3 Main Active ingredient Decapeptide-18 (CG-WINT) Decapeptide-28 (CG-Rehairin) Decapeptide-1 (CG-Keramin2) Octapeptide-11 (CG-Seperin) Oligopeptide-71 (CG-Edrin) Oligopeptide-54 (CG-Nokkin) Octapeptide-2 (Prohairin- DR.CYJ Hair Filler Mechanism Hair Growth Down Regulation of DKK1 Keratinocyte Migration Angiogenesis Anti -Apoptosis Hair Growth Fibronectin, Shh, Cdk Keratinocyte proliferation Anagen development Hair Stem Cell Proliferation (Hair stem cell differentiation) Anti-hair Loss (DDK-1 Down-regulation) Hair Growth Hair follicle formation Anti-hair Loss (Follistatin, CTGF/CCN2 as BMP inhibitor) Hair pigmentation 3

4 In-vitro Test Data 1. Expression of Hair Growth Related Genes Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells) Culture time: 24 hrs / Method: RT-PCR analysis HGF VEGF GAPDH Control 1ng ng 1ug Minoxidil Expression of growth factors related to growth of hair follicle by Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell We observed HGF and VEGF expression are regulated by treatment with Hair Filler Complex. Hair Filler Complex promotes hair growth through stimulatory effect on HGF and VEGF gene expression in HHFDPC. IDV( % of control) IDV( % of control) HGF 497 Control 1ng ng 1ug Minoxidil VEGF Control 1ng ng 1ug Minoxidil Expression of Hair Growth Related Proteins Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells) Culture time: 24 hrs Method: Western blot analysis IGF-1 FGF-7 Actin Control 1ng ng 1ug Minoxidil Expression of growth factors related to growth of hair follicle by Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell Hair Filler Complex promotes hair growth through stimulatory effect on IGF-1 and FGF-7 protein expression in HHFDPC. IDV( % of control) IDV( % of control) FGF-7 Control 1ng ng 1ug Minoxidil IGF Control 1ng ng 1ug Minoxidil Inhibition of DHT-induced Hair Loss Related Gene Expression Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells) Culture time: 24 hrs Method: RT-PCR analysis Stimulator: DHT 5ug/ml DKK-1 IL-6 TGF-"1 Inhibition of hair loss related gene expression DHT-induced expressions of hair loss-related genes, DKK-1, IL-6 and TGF-"1 were reduced by Hair Filler Complex treatment. 4. Inhibition of DHT-induced Cell Apoptosis Control ng 1ug Minoxidil DHT GAPDH Cell: HHFDP cell p6 (Human Hair Follicle Dermal Papilla Cells) Culture time: 3 days Method: SRB staining Stimulator: DHT 5ug/ml Relative rate ( % of control) Control ng ng 1ug Minoxidil DHT Cell growth assay after treatment of Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell Hair Filler Complex inhibits DHT-induced hair cell apoptosis Control DHT DHT+ng/ml Hair Filler Complex DHT+1ug/ml Hair Filler Complex DHT+Minoxidil

5 Human Clinical Study vs After * 1session every 2 weeks for 8weeks (4 sessions) Total Hair Density [1/cm 2 ] Hair Thickness [mm] CASE#1. Sex.M/Age: 46 CASE#2. Sex.M/Age: 45 CASE#3. Sex.M/Age: 47 CASE#4. Sex.F/Age: 38 CASE#6. Sex.M/Age: 28 After 2Months 28 Number Number Number Number Number % % % % Thickness (mm) Thickness (mm) Thickness (mm) Thickness (mm) Thickness (mm) % % 45% 35% 42% 17% After

6 [Published in June. 217] 217 Caregen Co.,Ltd

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