detergent Novozymes Mannaway New power for your detergent brand

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1 detergent Novozymes Mannaway New power for your detergent brand

2 Soups and sauces: Salad dressing, bearnaise sauce, chilli sauce, barbecue sauce, mayonnaise, ketchup, soup Desserts: : Ice cream, candy bars, frozen dessert, sorbet, whipped dessert topping Personal care products: Shampoo, body lotion, sun protection lotion, toothpaste

3 Mannaway The original and worldwide recognized mannanase Remove some of the most common stains on clothes with the power of Mannaway a bioinnovative detergent solution from Novozymes. Mannan stains, also known as gum stains, are hard to wash off and act like dirt attractors. It is difficult to avoid mannan stains as mannan is widely used as a thickener and stabilizer in sauces, salad dressings, ice creams, desserts, and beverages, as well as in various personal care products, including toothpastes, shampoos, conditioners, and lotions. After a wash, even if mannan-based stains might appear to have been washed out, the mannan is not and may even be distributed to otherwise clean clothes via the wash water. These invisible mannan residues on the clothes act as a kind of glue and attract soil particles, resulting in fabric graying and dirty clothes. What can Mannaway do for your brand? Mannaway is the original mannanase proven to remove mannan stains effectively and thus prevent fabric graying. Integrate Mannaway in your detergent formulations and make Mannaway the new power of your brand. Consumers around the world want their detergents to remove stains and keep the clothes clean and bright without Mannaway in their detergent, this is hard to achieve. Mannaway supports strong stain removal brand claims by effectively removing common tough stains Mannaway offers strong cleanness claims by removing the adhesive mannan Mannaway supports anti-graying brand claims by preventing soil deposition on mannan-stained fabric Mannaway is recognized for its exceptional performance and indepth cleaning, and helps you fulfill the demands of the market while enhancing the positioning and competitive power of your brand. Mannans constitute an increasing problem Mannan, also known as gum, is a carbohydrate found extensively in food products, household agents, and personal care products. Since more and more of these products around the world contain guar gum or locust bean gum, a trend that is on the rise, it is nearly impossible to keep clothing free from occasional mannan stains (Figure 1). Over 27,000 new products with mannans were introduced, globally, between 2001 and 2006 New products launched with mannans 8,000 7,000 6,000 5,000 4,000 3,000 2,000 1, Fig. 1. Increase in the number of new products containing mannans (guar gum or locust bean gum) launched worldwide from 2001 to Source: Mintel gndp, 2007

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5 What is mannan? Mannans are polysaccharides consisting of a mannose backbone linked together by b-1,4-linkages with side-chains of galactose, attached to the backbone by a-1,6-linkages (Figure 2). Two of the most commonly used mannans in food and personal care products are guar gum (E412) and locust bean gum (E410). The magic of Mannaway Enzymes are very specific, and carbohydrases like amylases and cellulases do not recognize the structure of mannan and can therefore not remove it from fabric. However, since mannanases, like Novozymes Mannaway, are galactomannan-specific carbohydrases, they are able to degrade the b-1,4-linkages in mannan and remove mannan stains from clothes. Mannaway breaks down mannans and effectively removes stains containing guar gum or locust bean gum, thereby increasing in-depth cleaning by removing the adhesive mannan. Galactose O O O α- 1,6-linkage O O O O O O O O O O O Mannose O O O O β-1,4-linkages Fig. 2. Illustration of a guar gum polymer. Mannaway degrades the b-1,4-linkages.

6 Improve stain removal with Mannaway Mannaway efficiently removes mannan-containing stains from a variety of food products, including chocolate ice cream, pudding, fruit sorbet, milkshake, sauces, and salad dressing. You can be confident that consumers will easily recognize the stain removal benefits when you add Mannaway to your detergent (stain pictures in Figure 3). The stain removal performance of Mannaway is documented in both powder and liquid detergents using commercially available EU powder detergents and US liquid detergents with and without Mannaway. When the detergent is dosed with just 0.4% Mannaway 4.0 T or Mannaway 4.0 L, the excellent stain removal effect is clearly visible (Figures 4 and 5). Two enzymes are better than one Add Mannaway to your detergent that contains Stainzyme, the high-performance amylase from Novozymes, and boost the stain removal power of your detergent. No matter if the stains are troublesome food stains containing starch or mannan you can exploit the synergy between these two enzyme solutions, leading to an elevated performance plateau (Figure 6). Unwashed Washed without Mannaway Washed with Mannaway Frij chocoloate ice cream Chocolate ice cream Chocolate pudding Locust bean gum Guar gum Fig. 3. Mannaway stain removal is clearly visible on a number of test swatches: Frij chocolate ice cream, chocolate ice cream (C-H009), chocolate pudding (EMPA 165), locust bean gum (CS-73), and guar gum (CS-43). (EU washing machine, 5 g/l, 40 C.)

7 EU: Mannan stain removal in powder detergents US: Mannan stain removal in liquid detergents EU color detergent + 0.4% Mannaway 4.0 T EU color detergent US detergent % Mannaway 4.0 L US detergent 1 US detergent % Mannaway 4.0 L US detergent 2 Remission Choc. ice cream (C-H009) Salad dressing (CS-06) Chocolate pudding (EMPA165) Guar gum (CS-43) Tesco Del Monte Frij Viennetta raspberry raspberry chocolate choc. sorbet smoothie milkshake ice cream Remission Choc. ice cream (C-H009) Salad dressing (CS-06) Chocolate pudding (EMPA 165) Guar gum (CS-43) Choc. ice cream (C-H033) Choc. ice cream (CS-68) Fig. 4. Mannaway provides a significantly improved stain removal effect (the higher the remission index, the cleaner the fabric) in a commercial EU color powder detergent on a range of stains from CFT, EMPA, Equest, and D&T. (EU washing machine, 5 g/l, 40 C.) Fig. 5. Mannaway provides a significantly improved stain removal effect (the higher the remission index, the cleaner the fabric) in commercial US liquid detergents on a range of stains from CFT and EMPA. (US top loader washing machine, 1.5 g/l, 20 C.) Mannaway boosts Stainzyme performance EU color detergent + 0.4% Mannaway 4.0 T + 0.4% Stainzyme 12 T EU color detergent + 0.4% Stainzyme 12 T EU color detergent + 0.4% Mannaway 4.0 T EU color detergent Remission Choc. ice cream (C-H009) Salad dressing (CS-06) Chocolate pudding (EMPA 165) Rice starch (CS-28) Corn starch (EMPA 161) Fig. 6. Mannaway provides efficient stain removal on chocolate ice cream containing mannan, while Stainzyme removes stains such as rice and corn starch. On stains containing both mannan and starch such as chocolate pudding and salad dressing, Mannaway has a significant stain removal effect, while Stainzyme alone is less effective. However, when combining the two enzymes, Mannaway degrades the mannan, giving Stainzyme access to the starch, thereby providing superior synergistic stain removal. (300 ml small-scale EU wash, 5 g/l, 40 C.)

8 Better and deeper cleanness Mannan binds strongly to cellulose fibers in cotton fabrics due to the high affinity between mannan and cellulose polymers. In addition, mannans have a glue effect on particulate soils released during a wash cycle or subsequent wear, resulting in dirty and gray clothes. Mannaway removes mannan stains and prevents soil deposition on mannan-stained clothes, thus preventing fabric graying. Regardless of whether it is a liquid or powder, a color or bleach-containing detergent, Mannaway adds its stain removal power to the detergent ensuring clean clothes. Fights aggressive dirt The cleaning effect of Mannaway can be demonstrated in the presence of a number of common outdoor soils that are likely to be present in everyday laundry such as traffic dirt, illustrated by the use of particulate soil such as carpet soil, and clay. To demonstrate how well Mannaway prevents soil deposition on mannan-stained fabrics, cotton T-shirts stained with guar gum and locust bean gum were washed in a commercial EU powder detergent and a US liquid detergent in the presence of carpet soil or clay. It is clear that cotton T-shirts washed with detergent containing no Mannaway are heavily soiled. This happens because clay or carpet soil particles glue onto the stains (on-spot measurement) as well as onto previously clean parts of the T-shirt (off-spot measurement). When Mannaway is present, the guar gum and locust bean gum stains are degraded, preventing clay and carpet soil particles from gluing to the fabric and ensuring that the fabric remains bright and clean (Figures 7 and 8).

9 Mannaway prevents soil deposition during wash in color and bleach, liquid and powder detergents EU: Mannaway anti-redeposition effect in powder detergent US: Mannaway anti-redeposition effect in liquid detergent EU detergent + 0.2% Mannaway 4.0 T EU detergent US detergent + 0.4% Mannaway 4.0 L US detergent Remission Off spot Guar gum (on spot) Locust bean gum (on spot) Remission Off spot Guar gum (on spot) Locust bean gum (on spot) Fig. 7. Mannaway is required to prevent soil deposition on cotton fabrics washed in powder detergents (the higher the remission index, the cleaner the fabric). If Mannaway is not added to the detergent when washing gum-stained fabrics in the presence of particulate soil (here carpet soil), the gum will glue carpet soil particles onto the stains on the T-shirts (on-spot measurement) as well as onto the previously non-stained parts of the T-shirts (off-spot measurement). Fig. 8. Mannaway is required to prevent soil deposition on cotton fabrics washed in liquid detergents (the higher the remission index, the cleaner the fabric). If Mannaway is not added to the detergent when washing gum-stained fabrics in the presence of particulate soil (here clay), the gum will glue clay particles onto the stains on the T-shirts (on-spot measurement) as well as onto the previously non-stained parts of the T-shirts (off-spot measurement). Mannaway prevents soil deposition when clothes are washed. If mannan stains (guar gum and locust bean gum) are not degraded by a mannanase and removed from the T-shirts, mannan soilings will smear and redeposit during the wash onto otherwise clean fabric surfaces. Here where they act as glue for soil particles, making the clothes dirty and gray. Without Mannaway, both on-spot / stained areas and off-spot / nonstained areas will experience deposition of soil (Figures 7 and 8). On spot (gum stains) Off spot 9

10 Mannans are everywhere In addition to the challenge of mannan in food products, more and more personal care products worldwide contain mannans in the form of guar gum or locust bean gum. According to an analysis from Mintel, 25% of all mannan-containing products launched between 2001 and 2007 were within the hair care and bath categories, making it hard to avoid mannan stains from lotions, toothpastes and shampoos (Figure 9). Mannaway ensures that mannan stains caused by many personal care products are removed, leaving clothes clean and bright. 25% of mannan-based products launched between were in the hair care and bath categories Desserts & Ice Cream 28% Haircare and Bath Products 25% Meals & Meal Centers 12% Dairy 10% Backery 8% Processed Fish, Meat & Egg Products 6% Sauces & Seasonings 4% Beverages 4% Snacks 3% Fig. 9. Relative number of products launched between 2001 and 2007 for different product categories containing mannans. Source: Mintel gnpd,

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12 Novozymes is the world leader in bioinnovation. Together with customers across a broad array of industries we create tomorrow s industrial biosolutions, improving our customers business and the use of our planet s resources. Novozymes A/S Krogshoejvej Bagsvaerd Denmark With over 700 products used in 130 countries, Novozymes bioinnovations improve industrial performance and safeguard the world s resources by offering superior and sustainable solutions for tomorrow s ever-changing marketplace. Tel Fax detergent@novozymes.com Main sales offices: Africa, Sub-Sahara Sandton, South Africa Tel Australia & New Zealand Sydney, Australia Tel Central & Western Europe Paris, France Tel Eastern Europe, Middle East & Northern Africa Vienna, Austria Tel Indian Subcontinent Bangalore, India Tel Japan Tokyo Tel Korea Seoul, South Korea Tel Mexico, Central America & Caribbean Mexico City, Mexico Tel North America Franklinton, North Carolina Tel Russia & Belarus Moscow, Russia Tel South America Araucária, Paraná, Brazil Tel South-East Asia Kuala Lumpur, Malaysia Tel China Beijing Tel Laws, regulations, and/or third party rights may prevent customers from importing, using, processing, and/or reselling the products described herein in a given manner. Without separate, written agreement between the customer and Novozymes to such effect, this document does not constitute a representation or warranty of any kind and is subject to change without further notice. Novozymes A/S Customer Communications No

13 DETERgent Independent test institute results Stain removal power of Mannaway proven by the internationally recognized textile research and service center Hohenstein Independent test institutes in Europe have developed many relevant stains that prove the benefits of Mannaway. The performance of Mannaway is therefore well recognized by both retailers and detergent producers. You can benefit from this performance too by adding Mannaway s stain removal power to your brand in both liquid and powder as well as color and bleach-containing detergents (Figure 10). Mannaway stain removal at Hohenstein test: EU color detergents EU detergent 1 EU detergent % Mannaway 4.0 T EU detergent 2 EU detergent % Mannaway 4.0 T EU detergent 3 EU detergent % Mannaway 4.0 T Very poor 5 PANEL SCORE (1 5) Performance Very good 1 Chocolate ice cream Mousse au chocolat Salad dressing Chocolate dessert Curry sauce Puddingzauber Mannaway stain removal at Hohenstein test: EU bleach-containing detergents EU detergent 1 EU detergent % Mannaway 4.0 T EU detergent 2 EU detergent % Mannaway 4.0 T EU detergent 3 EU detergent % Mannaway 4.0 T Very poor 5 PANEL SCORE (1 5) Performance Very good 1 Chocolate ice cream Mousse au chocolat Salad dressing Chocolate dessert Curry sauce Puddingzauber Fig. 10. Three color detergents and three bleach-containing powder detergents purchased at retailers were tested for the stain removal effect of adding Mannaway on top of the enzyme systems used today. The detergents were tested at the well-known European Hohenstein test institute using its standard range of mannan-based stains. On a majority of Hohenstein stains it is clear that even in detergents already containing a number of enzymes, Mannaway still has a significant effect on stain removal (EU washing machine, 5 g/l, 40 C). A similar effect will be seen in the case of liquid detergents. Novozymes A/S Customer Communications No