BOTANY Lab Manual BSc.-III Medical Semester V
|
|
- Morgan Ryan
- 5 years ago
- Views:
Transcription
1 BOTANY Lab Manual BSc.-III Medical Semester V 212
2 Experiment 1 Aim: Determine Water Potential of Vacuolar Sap by Plasmolytic Method. Requirements: Leaves of Tradescantia solutions of different concentrations, slides, microscope, coverslip, watch glass, blade brush etc. Theory: Plasmolysis is defined as shrinkage of protoplast of cells when they are placed in hypertonic solution. Osmotic potential is defined as the hydrostatic pressure which must be applied on solution to prevent further entry of water into it. OP = CRT [OP: Osmotic Potential, C: Concentration, R: Gas Constant, T: Temperature] 1. Peeled a small segment of epidermis from lower surface of leaf and placed in water. 2. Prepared different concentrations of sugar solution i.e. 0.1, 0.2, 0.3, 0.4 & 0.5 M. 3. Put 5 ml of different concentrations of sugar solution in respective watchglass and used water as control or blank. 4. Kept small pieces of epidermal peels in different sugar solutions for about 15 min. 5. Mounted different epidermal peels in their respective solution on clean glass slide and put coverslip. 6. Observed all peels under microscope and noted down observations. 1. Fully turgid leaves should be used for this experiment. 2. Sugar solution should be prepared carefully. 3. The epidermal peel should be single layered. 4. Observed different stages of Plasmolysis carefully. 213
3 Diagrams: Observations: Limiting Plasmolysis Incipient Plasmolysiss Evident Plasmolysis 214
4 Aim: Determine water potential of any tuber. Experiment 2 Requirements: Peeled Potato, Sugar solution of different concentrations, methylene blue, ten test tubes, pipette, dropper, cork borer, blotting sheet, blade, petridish etc. Theory: Water potential is the chemical potential of water which is equivalent to diffusion pressure deficiet (DPD) with negative sign. It is the difference between free enrgy of water molecules in pure water and that of water in a system or solution. It is equal to Ψ w = Ψ s + Ψ m + Ψ p [Where Ψ w is water potential, Ψ s is solute potential, Ψ m Matric potential and Ψ p Pressure potential] 1. Ten test tubes were taken and labeled as 0.1, 0.2, 0.3, 0.4 and 0.5 M in duplicate set. 2. Prepared different concentrations of sugar solutions viz. 0.1, 0.2, 0.3, 0.4 and 0.5 M in distilled water and put 10 ml of each concentration in two test tubes each. 3. Cut cylinders of potato tubers with the help of cork borer and trimmed to 1cm in length. 4. Dried potato tubers into folds of blotting sheet and immediately transferred two pieces in each test tube of one set. 5. Put a drop of methylene blue to same set and shook well. 6. Kept the set as such for about 30 min and a small drop of sugar solution from this set was withdrawn with the help of dropper and released in second tube of same concentration. 7. Observed the movement of drop carefully. 1. Potato pieces should be of equal length. 2. Sugar solutions should be prepared carefully. 3. The movement of drop should be observed against white background. 4. The tubes with potato tubers should be mixed thoroughly before taking observations. Observations 215
5 Concentration (M) Movement of Drop Nature of Solution Hypotonic Solution Hypertonic Solution (Drop moves down) (Drop moves upward) 216
6 Experiment 3 Aim: To study the extraction and separation of chlorophyll pigments by solvent method. Requirements: Pestle and mortar, Cassia leaf powder, separating funnel, conical flask, measuring cylinder, stand, filter paper, weighing balance, potassium hydroxide, acetone, petroleum ether, diethyl ether, distilled water, funnel, pipette, blotting sheet, glass rod. Theory: Chlorophylls are green coloured pigments present in the thylakoids of chloroplasts along with carotenoids. These can be extracted from leaves using organic solvents. 1. 8g of Cassia leaf powder was mixed with 100 ml of 80% acetone and stirred for 10 min. 2. Solution mixture was filtered twice using blotting sheet twice. 3. Transferred 40 ml of the filtrate in separating funnel and added 50 ml of petroleum ether along wall. 4. Rotated separating funnel gently for 5 min and added 60 ml of distilled water along sides of separating funnel. 5. Shook the mixture gently for 5-10 min and left undisturbed with the help of a stand till two layers separated. 6. Discarded lower layer and poured 50 ml of distilled water along sides, stirred gently and removed lower water layer carefully. 7. Added 40 ml of 92% methanol and mixed well. 8. Allowed separating funnel to rest for 2-3 min till two layers separated. 9. Removed lower layer in other separating funnel and performed further experiment for both layers separately. Upper layer: 10. Added 15 ml of 30% Methanolic KOH and 20 ml of distilled water along sides and shook well. 11. Two layers separated; upper light yellow coloured containing carotenes and lower bluish green containing chl a. 217
7 Lower layer: 12. Added 50 ml of diethyl ether to lower methanolic layer and shook well. 13. Added 20 ml of distilled water along sides and mixed well. 14. Allowed two layers to separate and added discarded lower methanolic layer. 15. Added 15 ml of 30% methanolic KOH and 20 ml of distilled water along sides carefully and shook separating funnel well. 16. Allowed two layers to separate; upper yellowish brown coloured containing xanthophylls and lower olive green coloured containing chl b. 1. The chlorophyll extract should be concentrated enough. 2. Distilled water should be added along sides to avoid emulsification. 3. Various reagents should be prepared carefully. 4. The separating funnel should be handled carefully. 5. Thorough mixing of the contents should be done before discarding any layer. Observations: Xanthophyll Chl b Carotene (Upper layer) Chl a (Lower layer) 218
8 Experiment 4 Aim: separation of pigments by paper chromatography. Requirements: Cassia leaves, 80% acetone, petroleum ether, 95% acetone, chromatography jar, whatman filter paper, pestle and mortar, aluminum foil, measuring cylinder, capillary tube, pencil, scale etc. Theory: Chromatography is a technique through which the components of a mixture are separated on the basis of colour differentiation. Most common methods include paper chromatography, which is two dimensional and various components are identified on the basis of Rf (Retention Factor) values: Rf = Distance travelled by organic substance Distance travelled by solvent front 1. Prepared a paste of cassia fresh leaves with 80% acetone. 2. Cut Whatman filter paper strip approximately 5 cm wide. 3. Draw a line 2 cm above one end of strip with the help of pencil as reference line. 4. Loaded concentrated solution of photosynthetic pigments in the centre of line with the help of capillary tube 8-10 times to increase the density of pigments. 5. Put solution of petroleum ether and 95% acetone in the ratio of 100: 12 in chromatography chamber. 6. Suspended the filter paper strip loaded with photosynthetic pigments in the jar with the help of aluminum foil. 7. The jar was left undisturbed for about 2 hours till the solvent front travelled enough to cover 2/3 rd of filter paper. 8. Took out the whatman filter paper strip and marked solvent front with the help of pencil. 9. Determined the distance travelled by various pigments from reference line and calculated Rf values. 1. The pigment extract should be concentrated enough. 2. The chromatogram should be developed till full separation of pigments. 219
9 3. The solvent mixture should not touch the spot of photosynthetic pigments. 4. The chromatographic chamber should be left undisturbed. 5. The filter paper should not touch the walls of chromatographic chamber. Observations: Calculations: Chromatogram 220
10 Experiment 5 Aim: Separation of amino acids in a mixture by paper chromatography. Requirements: Whatman filter paper, n-butanol, glacial acetic acid, chromatographic chamber, distilled water, alanine, lysine, 0.2% ninhydrin solution, capillary tubes, hot air oven, hot plate, weighing balance, aluminum foil, atomizer etc. Theory: Paper chromatography is a form of two dimensional chromatography where a mixture is separated into its components on a paper using a particular mixture of solvents as mobile phase. In this experiment amino acids are separated and visualized using ninhydrin which forms a blue coloured complex with them. 1. Cut Whatman filter paper strip of required size as the size of chromatographic chamber. 2. Prepared a mixture of n-butanol, glacial acetic acid and distilled water in the ratio of 3:1:1. 3. Poured the solvent mixture in chromatographic chamber and covered it with aluminum foil. 4. Draw a reference line of filter paper strip approx. 2 cm above the edge. 5. Spotted filter paper with pure amino acids and their mixture carefully about 8-10 times so as to load sufficient amount for chromatographic development. 6. Dried spots over hotplate and suspended the filter paper in TLC chamber carefully and left the set up undisturbed for about 2 hours. 7. Took out the strip and marked the level of solvent front. 8. Allowed filter paper to dry completely and sprayed with ninhydrin solution using an atomizer. 9. Heated the filter paper strip in oven at 90º C for about 5 min. 10. Marked the positions of amino acids and determined distance travelled by them from reference line. 11. Determined Rf values for amino acids according to formula. 1. Sufficient amino acids should be loaded on filter paper by repeated application. 2. Do not touch filter paper with hands to avoid contamination with amino acids in our sweat. 221
11 3. Don not dip spot of amino acids in mixture solvent. 4. Cover the chromatographic chamber with glass lid or aluminum foil to make the chamber saturated. 5. Determine Rf values carefully. Diagrams: Calculations: Rf = Distance travelled by amino acid Distance travelled by solvent front 222
12 Aim: To demonstrated Phototropism. Experiment 6 Requirements: Potted plant, phototropic chamber. Theory: Phototropism is defined as the plant curvature movement in response to light. Shoot is positively phototropic whereas root is negatively phototropic. 1. A wooden phototropic chamber painted black from inner side having a hole on one side with removable top was taken. 2. Placed a small potted plant inside this chamber. 3. Placed the chamber in sunlight or an artificial source of light. 4. Left the set up as such for few days. 5. Observed the orientation of different plant organs after few days. 1. The chamber should be painted black from inside. 2. The plant should be placed upright near the source of light. 3. There should be only one opening for entering light in the chamber. Diagrams: Observations: The plant shoot moved towards the source of light because shoot is positively phototropic. Experiment 7 Aim: Demonstrate Ascent of Sap using a dye. 223
13 Requirements: Fresh leafy shoot of balsam or petunia, blade, eosin dye, distilled water, scissor, stand, beaker, cotton, weighing balance, cotton etc. 1. A leafy shoot of petunia or balsam was cut under water obliquely. 2. Dipped the cut end in 2% eosin solution contained in a beaker. 3. Adjusted the shoot erect using a stand, cotton and left the set up undisturbed for an hour. 4. Observed the colour of veins on leaves, stem and flower after sometime. 5. Cut T.S of stem and V.S of leaf for observing the path of ascent of sap. 1. The leafy shoot should be cut under water obliquely. 2. Eosin solution should be prepared carefully. 3. The transverse section should be thin enough. Diagrams & Observations: Experiment 8 Aim: To demonstrate imbibitions by plaster of paris method. Requirements: Plaster of paris, water, petridish, funnel, filter paper, gram seeds, tripod stand, glass rod etc. 224
14 Theory: Imbibition is the process of adsorption of liquid on the surface of solid without forming solution. It generates huge imbibitions pressure designated by ψ m (Matric Potential). Its value may reach 1000 atm. 1. Prepared the slurry of plaster of paris using water and glass rod. 2. Poured the POP paste in glass funnel lined with filter paper to fill half. 3. Added number of dry gram seeds over it and more POP paste to cover them completely. 4. Allowed POP to harden for min. 5. The cone of POP was taken out along with filter paper, remover paper and placed cone in a petri dish filled with water with broad base downwards. 6. Observed POP cone after an hour. 1. The slurry should be made fresh and Seeds should be dry and viable. 2. The POP paste should be allowed to set properly before taking it out of funnel. Observations: 225
15 Experiment 9 Aim: To demonstrate the evolution of oxygen during photosynthesis. Requirements: Hydrilla plant, test tube, funnel, beaker, water, sodium bicarbonate, glass rod etc. Theory: Photosynthesis is the process of synthesis of carbohydrates from carbon dioxide and water in the presence of sunlight, chlorophyll and enzymes. This results in evolution of oxygen as byproduct which can be observed in the form of bubbles. 1. A fresh Hydrilla plant was taken and put in funnel. 2. Placed the funnel inverted in beaker containing distilled water. 3. Added a pinch of sodium bicarbonate in the beaker and mixed well with glass rod. 4. Inverted a test tube filled with water over the stem of funnel carefully. 5. Kept the set up in bright sunlight and observed carefully the evolution of bubbles after sometime. 1. Stem of the funnel should be completely immersed under water. 2. Place the test tube carefully over stem of funnel avoiding any spillage of water. 3. Only fresh Hydrilla plant should be used for this experiment. 4. The set up should be kept in sunlight for some time. 226
16 Diagrams: Observations: Hydrilla Plant Photosynthesizing 227
17 Experiment 10 Aim: To study the effect of different concentrations of organic solvents on permeability of plasma membrane. Requirement: Beet root, distilled water, acetone, test tubes, cork borer, knife, petri dish, blotting paper, beaker, measuring cylinder, scale, pipettes, distilled water etc. Theory: Plasma membrane is selectively permeable which allows the movement of some substances and not all. In response to different concentrations of organic solvents the membrane shows differential permeability. In this experiment cell membrane of beet root shows outward movement of anthocyanins, red coloured pigments from vacuolar sap due to injury caused by organic solvent in dose dependent manner. 1. The beet root cylinders of 1 cm length were cut using a cork borer and blade. 2. The cylinders were washed repeatedly using distilled water till the pigment stopped diffusing out. 3. Different concentrations of acetone i.e. 25, 50, 75 and 100% were prepared using distilled water. 4. The test tubes were filled with 10 ml of each concentration and water as control. 5. Put two pieces of beet root cylinders in each test tube and left the set up as such 30 min. 6. The observations were taken visually to determine maximum leaching of anthocyanins by comparing the intensity of colour. 1. The beet root pieces should be of equal length. 2. The concentration of organic solvents should be accurate. 3. Beet root pieces should be washed frequently till the leaching of pigment stops. 4. The test tubes should be shaken to allow maximum diffusion. Observations: 228
18 (% Conc. of acetone) Maximum leaching at 75% acetone 229
Experiment 11 Identification of Food Colors in Candies
Experiment 11 Identification of Food Colors in Candies Pre-lab Assignment Before coming to lab: Read the lab thoroughly. Answer the pre-lab questions that appear at the end of this lab exercise. Purpose
More informationCHM111 Lab Physical Separations Grading Rubric
CHM111 Lab Physical Separations Grading Rubric Name Team Name Criteria Points possible Points earned Lab Performance Printed lab handout and rubric was brought to lab 3 Safety and proper waste disposal
More informationExperiment #3. Physical Separations Candy Chromatography
Experiment #3. Physical Separations Candy Chromatography Goals 1. To physically separate and identify dyes in candy by comparison to commercial food dyes using paper chromatography. 2. To become familiar
More informationPaper Chromatography and Steam Distillation EVERY STUDENT MUST BRING AT LEAST 3 ORANGES TO LAB FOR THIS EXPERIMENT! Equipment
Paper Chromatography and Steam Distillation EVERY STUDENT MUST BRING AT LEAST 3 ORANGES TO LAB FOR THIS EXPERIMENT! Equipment You will need a 600 ml beaker, a 50 ml graduated cylinder, 4 Expo Wet Erase
More informationCHEM 008 Experiment 5 CHROMATOGRAPHY. Text Topics and New Techniques. Discussion and Techniques. Column and paper chromatography, visible spectroscopy
CHEM 008 Experiment 5 Fig. 5-1 CHROMATOGRAPHY Text Topics and New Techniques Column and paper chromatography, visible spectroscopy Discussion and Techniques One of the most important aspects of chemistry
More informationEXTRACTION OF ANTHOCYANIN PIGMENTS FROM RED APPLE SKIN, EGGPLANT SKIN, RADISH SKIN, AND
LEARNING ABOUT PLANT COLORS AND PIGMENTATION Developed by Julia Dupin, 2017 This activity was designed to showcase the diversity of pigment types in plants and show how they can be extracted from plant
More informationnames 1 inch + Black Vis-à-Vis Black Sharpie
Types of Covalent Compounds: Polar and Nonpolar If you ever had a piece of paper get wet, you ve noticed that the ink making up the lines of the paper or the ink from your carefully collected notes travel
More informationLaboratory technique and preparations
Laboratory technique and preparations Bio 381 written by : Hind Alzaylaee Alshareef_ Maryam Alzayn Alshareef 9/17/2012 graduated cylinder Funnel Flask beaker Dropping bottle Watch glass Petri dish Reagent
More informationName: Date: Period: Can I eat that? Lab
Name: Date: Period: Can I eat that? Lab Objective Part 1 Engage Your teacher is allergic to blue dye that is used in many foods and products. Your objective is to determine what colors of M&Ms or skittles
More informationWHAT IS GEL ELECTROPHORESIS?
Getting Started With Gel Electrophoresis a world of learning Presented by Peter J Ball, Southern Biological. For further information, please contact the author by phone (03) 9877-4597 or by email peterjball@southernbiological.com.
More informationWARNING. Not suitable for children under 8 years. For use. WARNING This set contains chemicals that may be harmful if EXPERIMENT MANUAL
EXPERIMENT MANUAL Please observe the safety information below, the advice for supervising adults on page, the safety rules on page, and the information about hazardous substances (chemicals) and their
More informationThe Identification of a Lipstick Brand: A Comparison of the Red Pigment R f Values using Thin Layer Chromatography
The Identification of a Lipstick Brand: A Comparison of the Red Pigment R f Values using Thin Layer Chromatography Ali Robertson and Margaret Mercer Heathwood Hall Episcopal School 11 th Grade 1 ABSTRACT
More informationLeaf Chromatography. -Community Resources for Science
Thank you for downloading the science and mathematics activity packet! Below you will find a list of contents with a brief description of each of the items. This activity packet contains all the information
More informationWhat is Life? Project PART 1: Looking at Cells Lab
What is Life? Project PART 1: Looking at Cells Lab Directions: Complete the drawings and answer the questions in the space provided. For each drawing: Title the drawing of the specimen (e.g. Cork Cells)
More informationFranklin Regional School District SCIENCE MIDDLE SCHOOL Bid #16 Vendor Specifications
SCIENCE MIDDLE SCHOOL Bid #16 Quotation number: (Number you want reflected on the Purchase Order) Company: Address: Telephone: Email: Date: Authorized name (print): Authorized Signature: 1 GLASSWARE/TUBING/CYLINDERS
More informationKERATIN CONTAMINATION
KERATIN CONTAMINATION Keratin contamination is almost always observed as a background protein. Wear only nitrile gloves and rinse with HPLC grade water all trays, containers and surfaces that contact the
More informationSTUDENT LABORATORY PACKET
L5 Elodea-Onion-Cheek-Cell_Size Page 1 of 7 STUDENT LABORATORY PACKET Student s Full Name Lab #5: Elodea, Onion, Cheek Cells-Cell Size Lab Instructor Date Points Microscope # OBJECTIVES: a. to examine
More informationEXERCISE 8C - Lab Procedures
EXERCISE 8C - Lab Procedures SAFETY WARNING: Acrylamide in the unpolymerized form is a skin irritant and a potential neurotoxin. Fortunately, the acrylamide in your gels is polymerized, so it should not
More informationForensic examination of lipstick by the various physio-chemical and instrumental method.
Forensic examination of lipstick by the various physio-chemical and instrumental method. Sapana Singh; Vaibhav Saran; Munish Mishra, AK Gupta M.SC Forensic Science ; Assistant Professor; Assistant Professor;
More informationThin Layer Chromatography for Forensic Science
Parkland College A with Honors Projects Honors Program 2014 Thin Layer Chromatography for Forensic Science Kseniya Chumachenko Parkland College Recommended Citation Chumachenko, Kseniya, "Thin Layer Chromatography
More informationIntroductory Chemistry
Introductory Chemistry Lab 1: Introduction and Safety Objectives Learn how work to safely in the chemical laboratory Learn when and how to use the safety equipment in the chemical laboratory Learn the
More informationANALYSIS OF FINGERPRINTS, LIPSTICK 2 ND HAIR
ANALYSIS OF FINGERPRINTS, LIPSTICK 2 ND HAIR LAB FORENSICS.3 From Sourcebook, National Science Foundation, 1997 INTRODUCTION PART A. OBTAINING A FINGERPRINT Black ink stamp pad Tissue paper 4 x 4 cm Card
More informationStaining of the clinical material or the bacteria from colonies on laboratory media provide a direct visualization of the morphology of the organisms
COMMON STAINING PROCEDURES Staining of the clinical material or the bacteria from colonies on laboratory media provide a direct visualization of the morphology of the organisms as well as their reactions
More informationStandard Laboratory Practice for Consumer Applied Pet Stain and Odor Removal Chemical Evaluation on Pile Yarn Floor Coverings
P.O. Box 2048 Dalton Georgia 30722-2048 706.278.3176 carpet-rug.org CRI Test Method - 116 Technical Bulletin Standard Laboratory Practice for Consumer Applied Pet Stain and Odor Removal Chemical Evaluation
More informationPET Barrier Test PET- R- 02
PET Barrier Test PET- R- 02 The following protocol is designed to provide a procedure for identifying and quantifying residual amounts of three barrier materials, EVOH, MXD6 nylon, and epoxy diamine, in
More informationDRAFT EAST AFRICAN STANDARD
DEAS 341: 2012 ICS 71.100.70 HC 3304 99 20 DRAFT EAST AFRICAN STANDARD Nail polish removers Specifications EAST AFRICAN COMMUNITY EAS 2012 First Edition 2012 DEAS 341: 2012 Copyright notice This EAC document
More informationBasic Microbiology and Immunology Practical Course
Basic Microbiology and Immunology Practical Course 2 Lab # 2: Colouring the microorganisms Rules that must be followed to maintain an aseptic zone 3 For most bacterial cultures, you will use a sterile
More informationPROTOCOLS FOR ANATOMY/MICROMORPHOLOGY
PROTOCOLS FOR ANATOMY/MICROMORPHOLOGY General dissection of spikelets... 2 Hand sections and epidermal scrapes of bamboo leaves... 2 Clearing and staining of intact plant organs... 4 Scanning electron
More informationPrisma & Film Staining Workshop. Application Specialist Mea Pelkonen
Prisma & Film Staining Workshop Application Specialist Mea Pelkonen Tissue-Tek Prisma Tissue-Tek Prisma Always program the Prisma in the following order: 1. Edit solution names Check if desired solution
More informationExperiment 6. Paper Chromatography. Chemicals and hazard statements
Experiment 6 Paper Chromatography Chemicals and hazard statements 1. 0.5 M Manganese (II) chloride (MnCl 2 ). Harmful if swallowed. Harmful to aquatic life with long lasting effects. Wash skin thoroughly
More informationStudent Performance Guide. Student Performance Guide. Student Performance Guide
LESSON 8-2 Collecting and Processing Specimens for Parasite Examination Student Performance Guide LESSON 8-3 Microscopic Methods for Student Performance Guide LESSON 8-4 Preparing and Staining Smears for
More informationComparative Proteomics Kit II: Western Blot Module Quick Guide
Comparative Proteomics Kit II: Western Blot Module Quick Guide Lesson 1 1 Label one 1.5 ml fliptop micro tube for each of five fish samples. Also label one screw-cap micro tube for each fish sample. 2.
More informationPhenion FT Skin Model Histological processing Paraffin sections
Phenion FT Skin Model Histological processing Paraffin sections Objective This Standard Operation Procedure is recommended to fix and embed Phenion FT Skin Models in order to prepare paraffin sections.
More informationCrime Busters. Lin Wozniewski
Crime Busters Lin Wozniewski lwoz@iun.edu Safety Students must wear: Closed shoes All skin from neck to toes covered Lab coat or lab apron Indirect vent or unvented chemical splash proof goggles. All skin
More informationObserving Moss and Cheek Cells
Observing Moss and Cheek Cells Name: Block: Partner(s): Date:!! SAFETY PRECAUTIONS!! Methylene blue solution will stain clothes and skin. Broken slides should be handled with paper towels, not bare hands.
More informationWet/ Tank Blotting System
A Geno Technology, Inc. (USA) brand name Wet/ Tank Blotting System Cat. No. BT301 1-800-628-7730 1-314-991-6034 info@btlabsystems.com SPECIAL NOTE: Thanks for choosing BT Lab Systems BT301 Wet/ Tank Blotting
More informationPolymer Bonded Explosives XTX 8004 (80 wt% PETN-20 wt% PDMS)
Intro/Motivation Polymer Bonded Explosives XTX 8004 (80 wt% PETN-20 wt% PDMS) Mock Energetic Composite Fabrication Procedure Engin C. Sengezer Department of Aerospace and Ocean Engineering, Virginia Tech,
More informationLab. Elodea, Onion, and Cheek Cell Lab. Be your best. Cell Biologist s Name: Period: Date: Mrs. Bouchard -7 th Grade Science
Purpose Ques*on: How do plant cells and animal cells differ? Cheek Cell Lab Materials: Water bo6le with dropper toothpick glass slide coverslip lens paper methylene blue safety goggles lab apron paper
More informationLab Six:- Medical Microbiology Prepared by: Luma J. Witwit. Staining
Staining Even with the microscope, bacteria are difficult to see unless they are treated in a way that increases contrast between the organisms and their background. The most common method to increase
More informationStudent Manual SO 3 N N N + Blue 1 Yellow 5 N N. Yellow 6 Red 40. Fig. 3. Reference dyes used in this experiment. - O 3 S - OOC
Student Manual Background How do you pick the foods you eat? The appearance of the food is a major deciding factor. For example, color can tell you whether a fruit is ripe or whether meat has spoiled.
More information2.6-1 SCIENCE EXPERIMENTS ON FILE Revised Edition. Cloud Chamber
2.6-1 SCIENCE EXPERIMENTS ON FILE Revised Edition Cloud Chamber Topic Cloud formation Time 1 hour! Safety Please click on the safety icon to view safety precautions. Do not touch dry ice with bare hands.
More informationStudent Manual SO 3 N N N + Blue 1 Yellow 5 N N. Yellow 6 Red 40. Fig. 3. Reference dyes used in this experiment. - O 3 S - OOC
Student Manual Background How do you pick the foods you eat? The appearance of the food is a major deciding factor. For example, color can tell you whether a fruit is ripe or whether meat has spoiled.
More informationWestern Blotting Systems CAT NO:EPS-B0015 & EPS-B0016
Western Blotting Systems CAT NO:EPS-B0015 & EPS-B0016 TABLE OF CONTENTS Important User Information Section 1 General Information 1.1 Introduction 1.2 Standard supply 1.3 Specifications Section 2 Instructions
More informationE-Blotter Operation. Technical Bulletin E-03 MATERIAL PROCEDURE
E-Blotter Operation MATERIAL BSA (1 mg/ml; 0.05 g BSA (Sigma-Aldrich Ltd., St Louis, MO; U.S.A.) dissolve in 50 ml ddh 2 O, aliquot to 1 ml in micro-centrifuge tubes and freeze in -20 C) NK92 cell lysate
More informationAcid Or Alkali? Testing With Cabbage
Acid Or Alkali? Testing With Cabbage Topic Using vegetables as an acid/base indicator Introduction Forensic scientists need to discover if someone has tampered with liquids (e.g., cosmetics, cleaning products,
More informationLAB 3 CHARACTERIZING YOUR UNKNOWN BACTERIA AND USING MORE COMPLEX STAINS. Part I: Isolating Your Unknown Bacteria and Describing Colony Morphology
LAB 3 CHARACTERIZING YOUR UNKNOWN BACTERIA AND USING MORE COMPLEX STAINS Objectives In this lab you will learn how to: - describe bacteria on the basis of colony and cell morphology - isolate bacterial
More informationLiposomal vitamin C highly concentrated for topical application with SDS system. Restores the physical and mechanical properties of the skin.
DERMALIFT Facial Care Liposomal vitamin C highly concentrated for topical application with SDS system Restores the physical and mechanical properties of the skin. Intense, fast, profound and lasting e
More informationUnit Introduction. Solutions, Mixtures, and Emulsions Vocabulary N A M E
Unit Introduction Vocabulary chemist a person who studies solids, liquids, and gases to solve problems chemistry the branch of science that identifies the substances that compose matter; the study of the
More informationSOUND. What can I learn through Integrated curriculum play explorations?
What can I learn through Integrated curriculum play SOUND I can see sound waves move water. I can feel it through he speakers. I can hear it. I can see the picture of the anatomy of an ear. I can explore
More informationOptiblot SDS-PAGE Gel
Instructions for Use For the use in SDS-PAGE with precast gels This product is for research use only and is not intended for diagnostic use. www.abcam.com 1 Table of Contents Optiblot SDS-PAGE Gel 1. Introduction
More informationStains and Solutions Used in Hematology and Cytology
Stains and Solutions Used in Hematology and Cytology A APPENDIX Acid-Fast Stain Commercially prepared acid-fast stains are available 1. Ziehl Neelsen carbolfuchsin: Dissolve 3.0 g basic fuchsin in 100
More informationWhat safety precautions have to be taken when handling alkalis?
Science - Chemistry - Acids, Bases, Salts - 2 Bases (P7158600) 2.1 Safety precautions to be taken when handling alkalis Experiment by: Phywe Printed: Oct 15, 2013 1:44:12 PM intertess (Version 13.06 B200,
More informationRubber Insulating Gloves Care/Storage/Inspection
Index/Procedure Steps: GENERAL INFORMATION... 2 MARKINGS... 2 MANUFACTURER S IDENTIFICATION... 2 PRE-USE INSPECTION... 3 VISUAL INSPECTION... 3 AIR TEST... 4 CARE AND STORAGE... 5 PERIODIC ELECTRIC PROOF
More informationUnit 3 Hair as Evidence
Unit 3 Hair as Evidence A. Hair as evidence a. Human hair is one of the most frequently pieces of evidence at the scene of a violent crime. Unfortunately, hair is not the best type of physical evidence
More informationDevelopment of specialty paper is an art: Titanium dioxide loaded poster from indigenous raw material Part X
Indian Journal of Scientific & Industrial Research Vol 63, May 2004, pp 420-424 Development of specialty paper is an art: Titanium dioxide loaded poster from indigenous raw material Part X Dharm Dutt *,
More informationOptiblot Non-Reducing Electrophoresis Kit
Optiblot Non-Reducing Electrophoresis Kit Instructions for Use For the use in non-reducing SDS-PAGE with precast gels This product is for research use only and is not intended for diagnostic use. 1 Table
More informationOptiblot SDS-PAGE Gel
Optiblot SDS-PAGE Gel Instructions for Use For the use in SDS-PAGE with precast gels This product is for research use only and is not intended for diagnostic use. 1 Table of Contents 1. Introduction 3
More informationStudent Performance Guide. Student Performance Guide. Student Performance Guide. Student Performance Guide. LESSON 3-3 Bleeding Time
LESSON 3-3 Bleeding Time Student Performance Guide LESSON 3-4 Prothrombin Time Student Performance Guide LESSON 3-5 Activated Partial Thromboplastin Time Student Performance Guide LESSON 3-6 Rapid Tests
More informationSureCast Handcast System For preparation of handcast mini gels for use with the Mini Gel Tank and XCell Surelock systems
Handcast System For preparation of handcast mini gels for use with the Mini Gel Tank and XCell Surelock systems USER GUIDE Catalog Numbers HC1000, HC1000S, and HC1000SR Publication Number MAN0014073 Revision
More informationTeacher and Technician Sheet
Teacher and Technician Sheet In this practical students will: Learn and use key terms, such as, suspension, reaction, pigment, opacity and tack. Create and use their own inks. Evaluate the effects of various
More informationOptiblot SDS-PAGE Gel
Optiblot SDS-PAGE Gel Instructions for Use For the use in SDS-PAGE with precast gels This product is for research use only and is not intended for diagnostic use. 1 Table of Contents 1. Introduction 3
More informationCosmetic Chemistry Developed for CWSE-ON 2010
Cosmetic Chemistry Developed for CWSE-ON 2010 Melanie Veltman & Rebecca Swabey 1 2 A Crazy Cosmetic Chemistry Vocabulary Game Fatty Acid: Points: Coal Tar: Points: Esters: Points: Boric Acid: Points: 3
More informationPreparation of Ink. Abstract
1 Preparation of Ink Abstract To Study the Preparation of Ink. This project throws a light on types of inks from manufacturing point of view and includes method for preparing them either in chemistry laboratory
More informationGlycoprotein Staining Kit
193PR 02 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Glycoprotein Staining Kit With Rapidstain for Enhanced Glycoprotein & Non Glycoproteins
More informationfor Stool Examination Issued by: LABORATORY MANAGER Original Date: March 13, 2000 Approved by: Laboratory Director Hematoxylin Stain
Section: Page 28 Policy # MI\PAR\05\06\v01 Page 1 of 5 Subject Title: Laboratory Procedures for Stool Examination Issued by: LABORATORY MANAGER Original Date: March 13, 2000 Approved by: Laboratory Director
More informationRittel s EZ-100 TANNING INSTRUCTIONS
Rittel s EZ-100 TANNING INSTRUCTIONS RITTEL S EZ-2000 Kit Using EZ-100 the newest and highest quality tanning agent available and only from RITTEL S and our authorized Distributors! This is a powdered
More informationSpot-Cleaning Tips and Remedies
The best and most convenient way to remove spots and spills is to have your carpet serviced by an Aladdin Professional. However, the following tips and home remedies should help remove most spots and odors.
More informationPreparing the Gel Box and Pouring the Agarose Gel
Preparing the Gel Box and Pouring the Agarose Gel Student Workstation Quantity Plastic chamber 1 8-well comb 1 Ruler 1 Molten agarose 50 ml Marking pen 1 Protocol 1. Using a ruler, measure the length,
More informationMt. San Antonio College: Spring 2018 MICR 22 Lab Orientation. Welcome to the Microbiology 22 Laboratory!
Mt. San Antonio College: Spring 2018 MICR 22 Lab Orientation Welcome to the Microbiology 22 Laboratory! Laboratory Objectives: To teach concepts of microbiological techniques using critically selected
More informationROBOT PIN TOOL CLEANING AND LIQUID SAMPLE TRANSFER
OVERVIEW TECHNICAL NOTE 67B ROBOT PIN TOOL CLEANING AND LIQUID SAMPLE TRANSFER There are several key steps in the successful use of pin tools: 1. The first and most important step is to start with clean
More informationDetermining the Effects of Age of Stain on Stain Removal Annabel Winterberg, Skye Murray October 3rd Introduction
Determining the Effects of Age of Stain on Stain Removal Annabel Winterberg, Skye Murray October 3rd 2014 Introduction The purpose of this experiment was to determine the effect of the age of a stain on
More informationSurgical Gown. Tongue Depressor. A disposable gown worn by medical staff during surgery. A thin, flat, wooden stick rounded at both ends
Tongue Depressor A thin, flat, wooden stick rounded at both ends Accidentally dropped on the floor by the doctor 16 Surgical Gown A disposable gown worn by medical staff during surgery Used by the surgeon
More informationLIQUID SOAP INTRODUCTION
LIQUID SOAP The hygiene is important for people to keep clean and at the same time to avoid infections and other kind of diseases. This soap is used exclusively by hands. Objectives Drawing a simple of
More informationSection F - Subsection 8 Hungarian Red Page 36
36 THIS PROCEDURE HAS NOT BEEN APPROVED FOR USE. NOTIFICATION OF APPROVAL WILL BE PROVIDED AT A LATER DATE. Name of Procedure: Hungarian Red Suggested Uses: Hungarian Red may be utilized in conjunction
More informationAN INTRODUCTION TO METHODS OF STUDYING THE MORBID HISTOLOGY OF DISEASE-CARRYING INSECTS.
243 AN INTRODUCTION TO METHODS OF STUDYING THE MORBID HISTOLOGY OF DISEASE-CARRYING INSECTS. By CAPTAIN A. E. HAMERTON, D.S.O. Royal Army Medical Oorps. THE great technical improvements in modern histological
More informationBIOL 251 BASIC MICROBIOLOGY
BIOL 251 BASIC MICROBIOLOGY CHARACTERISATION OF BACTERIA CHARACTERISATION OF BACTERIA CHARACTERISATION OF BACTERIA MICROSCOPIC To be able to examine microbes microscopically, they need to be stained
More informationInvestigator Guide to Studying Hair
Investigator Guide to Studying Hair Hair is a great subject for anyone to study; there is so much of it around. You can find it on people s heads; coming off from your pets when you stroke them; in your
More informationSeries cc. Series 7. Type Series 7. trimmer. Series cc. off. sensitive. intensive. clean. high. low. empty.
on Series 5 790 cc 790 cc ing sensitive intensive reset Type 5692 on on Ser Ser 3 4 4a 8 9 ing 10 2 1 5 6 790 cc 11 sensitive reset intensive 12 13 14 15 7 16 17 20 18 19 sensitive on on reset intensive
More informationCandidate. Number Other Names
Centre Number Surname Candidate Number Other Names For Examiner s Use Total EMPA mark Notice to Candidate. The work you submit for assessment must be your own. If you copy from someone else or allow another
More informationBIO 611 Polarity and Mixing Lab
Name: Date: Block: Introduction: BIO 611 Polarity and Mixing Lab When you mix two substances together, the first thing they do is decide whether or not they like each other. If they do, one will usually
More informationEXPERIMENT. Bacterial Morphology and Staining Techniques
EXPERIMENT Bacterial Morphology and Staining Techniques Hands-On Labs, Inc. Version 42-0240-00-02 Review the safety materials and wear goggles when working with chemicals. Read the entire exercise before
More informationSeries7. Series 7. trimmer. Series 7. off. sensitive. intensive. clean. high. low. empty. reset
on Series7-3 & dry fast sensitive off intensive reset www.braun.co.jp 2 3 4 5 6 7 on on sensitive off off intensive reset 8 off Series Series & dry fast sensitive on on reset intensive 9 on sensitive off
More informationCarpet Cleaning Guide. Carpet Cleaning 101: An Overview
Carpet Cleaning Guide Carpet Cleaning 101: An Overview Step One: Identify Carpet Fiber Type Cotton Wool, Silk Nylon Olefin Polyester Acrylic Synthetic Composition Cellulosic Cotton Seed, Jute Protein Based-
More informationBig Pieces or Small Piece: Which React Faster?
Chemistry Big Pieces or Small Piece: Which React Faster? Objective (from http://www.sciencebuddies.com) The goal of this project is to measure the effect of reactant particle size on the rate of a chemical
More informationThe MiniOne TM Reagent Kit: Crime Scene Invesitigation
The MiniOne TM Reagent Kit: Crime Scene Invesitigation Cat#: M3005 2 Crime Scene Investigation Reagent Kit Components Other Required Materials Laboratory Safety Table of Contents Page 3 3 3 Experiment
More informationVisPRO 5 Minutes Protein Stain Kit
Manual VisPRO 5 Minutes Protein Stain Kit VP01-125/VP01-500/VP05-125/VP05-500 V2.0 Store at room temperature For Research Use Only Introduction VisPRO 5 Minutes Protein Stain Kit (1 nanogram grade) provides
More informationAlkalis are contained in what substances and what is their purpose? Logged in as a teacher you will find a button below for additional information.
Science - Chemistry - Acids, Bases, Salts - 2 Bases (P7158700) 2.2 Alkalis - constituents of household detergents Experiment by: Phywe Printed: Oct 15, 2013 1:45:25 PM intertess (Version 13.06 B200, Export
More informationTECHNICAL INFORMATION Master Questioned Document Kit Catalog No. MQDA500
SIRCHIE Products Vehicles Training Copyright 2012 by SIRCHIE All Rights Reserved. TECHNICAL INFORMATION Master Questioned Document Kit Catalog No. MQDA500 INTRODUCTION The MQDA500 is one of the most complete
More informationHoefer SE615 and SE675
user manual Hoefer SE615 and SE675 Multiple gel casters um SE615-IM/Rev.C0/08-12 Page finder Safety warnings and precautions...viii Introduction...1 Setting up the caster...5 Casting with divider plates...7
More informationcollagen 360º After years of research, mesoestetic presents the result of the latest advances in cosmeceuticals and nutricosmetics
After years of research, mesoestetic presents the result of the latest advances in cosmeceuticals and nutricosmetics HEALTH + AESTHETICS The cosmetics sector is moving fast. It constantly includes new
More informationConversion of sugarcane by product (filter cake mud) from Savannah sugar company Nigeria, into commercial paste shoe polish
Journal of Advanced & Applied Sciences (JAAS), 1 (1): 11-19, 2013 2013 Academic Research Online Publisher. Research Paper Conversion of sugarcane by product (filter cake mud) from Savannah sugar company
More informationProsonic TM. 790 cc. Type trimmer. off. eco. normal. clean. intensive. high. auto select. low. empty. reset
on Prosonic TM Prosonic TM 790 cc off reset Type 5671 Braun GmbH Frankfurter Straße 145 61476 Kronberg/Germany 5-671-363/00/IV-07/M China/GB on reset 3 4 4a 8 5 9 10 2 1 6 11 12 off reset 13 7 14 15 18
More informationPREPARATION OF BLOOD FILMS FOR MALARIA DETECTION
PREPARATION OF BLOOD FILMS FOR MALARIA DETECTION Materials for Preparation of Malaria Smears: Clean and wrapped slides Sterile lancets 70% ethanol and water Absorbent cotton wool Surgical gloves Lint-free
More informationA New Method for Staining Connective Tissue Fibres, with a Note on Liang's Method for Nerve-fibres. By G. OWEN
421 A New Method for Staining Connective Tissue Fibres, with a Note on Liang's Method for Nerve-fibres By G. OWEN (From the Department of Zoology, The University, Glasgow) With two plates (figs, i and
More informationEnviron presents Professional training
Environ presents Professional training Environ Skin Care (Pty) Ltd 2014 Product penetration the Environ difference Remember the functions of the skin: S.H.A.P.E.S. the epidermis Epidermis: 0.1 0.2 mm thick
More informationLaboratory Orientation. Biological Screening
Laboratory Orientation Laboratory Orientation Safety Clean technique Reagent preparation Use of basic equipment Quality assurance : Laboratory Orientation 2 Safety National Forensic Science Technology
More informationPROTOCOL FOR SCIENCE EQUIPMENT USAGE WITH EMPHASIS ON BS240/242: MICROORGANISMS AND THEIR HUMAN HOSTS Updated July 2015
PROTOCOL FOR SCIENCE EQUIPMENT USAGE WITH EMPHASIS ON BS240/242: MICROORGANISMS AND THEIR HUMAN HOSTS Updated July 2015 Personnel Maija Bluma (573-651-2069; mbluma@semo.edu) is the Microbiology Preparation
More informationSeries 5. Series cc. Type Series 7. trimmer. Series 7. off. 760 cc. sensitive. intensive. clean. reset
on Series 5 760 cc 760 cc ing sensitive intensive reset Type 5693 on Series 3 4 8 9 ing 10 2 1 5 6 760 cc 11 sensitive intensive 12 13 14 reset 15 7 16 17 20 18 19 760 cc sensitive on on reset intensive
More informationSteps of microbial smear preparation :
Lab 4 STAINING Practical Microbiology Microbial smear : It is a very small amount of microbial growth ( broth or solid ) spreaded on a clean slide and drying by air. Fixation : The process of passing the
More information: In order to study tissues with a microscope they must be preserved (fixed)- fixation Following fixation, blocks of tissue must be cut into thin
: In order to study tissues with a microscope they must be preserved (fixed)- fixation Following fixation, blocks of tissue must be cut into thin sections.-microtomy Other techniques involve dehydration
More information