University Of Florida. Bloodborne Pathogen Program. Standard Operating Procedures
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1 University Of Florida Bloodborne Pathogen Program Standard Operating Procedures Revised February 9, 2011 Updated (annually) BBP Standard Operating Procedures Page 1 of 13
2 University Of Florida Bloodborne Pathogen Program Standard Operating Procedures Please use the enclosed worksheets to create standard operating procedures (SOPs) for individual laboratories or work areas. These pages are intended to be photocopied and used as a means of compliance with the bloodborne pathogen standard. These worksheets will assist you in tailoring the Exposure Control Plan to your individual needs. Please note that they are to be used in conjunction with the UF Exposure Control Plan. Keep the completed worksheets with the Exposure Control Plan in a location that is accessible to your employees. EH&S will monitor individual laboratories for the presence of SOPs. There is no need to send the completed sheets to EH&S. Table of Contents Facility Identification Sheet... 3 JOBS: Exposure Determination List... 4 TASKS: Exposure Determination List... 5 HBV Vaccine... 6 Exposure Management... 7 Protective Equipment Used... 8 Decontamination Schedule... 9 Engineering Controls Check Biomedical Waste Plan BBP Standard Operating Procedures Page 2 of 13
3 University of Florida Exposure Control Plan Standard Operating Procedures Facility Identification Facility Name Neurochemistry Core Facility Room & Building # P2-32 & P2-41 Pharmacy Building 445 Mailing Address JHMC Box Phone Number or Plan Prepared By Dr. William J. Millard Date February 18, 2009 Date of Review/Update February 9, 2011 Signature of supervisor William J. Millard Name of supervisor (PLEASE PRINT OR TYPE) _Pharmacodynamics Department (PLEASE PRINT OR TYPE) BBP Standard Operating Procedures Page 3 of 13
4 Exposure Determination List Jobs The following persons and job classifications in this facility may have contact with blood or other potentially infectious materials. These people are in the UF BBP program. (Please use official UF job classification titles). NAME JOB CLASSIFICATION William J. Millard Professor Yun-Ju He Senior Biological Scientist BBP Standard Operating Procedures Page 4 of 13
5 Exposure Determination List Tasks The following procedures performed in this facility may result in occupational exposures to blood or other potentially infectious materials. (Please list all tasks in which exposure may occur). Radio-immunoassay Procedures Sample Pipetting and aliquotting Enzyme linked Immunosorbant Assay BBP Standard Operating Procedures Page 5 of 13
6 Hepatitis B Vaccine Write in the names of your employees, with the date they were offered the hepatitis B vaccine. The following at-risk employees have been offered the hepatitis B vaccine free of charge: Name Date provided Date declined William J. Millard 1/94, 2/94, 7/94 Yun-Ju He 6/93, 10/94, 8/96 BBP Standard Operating Procedures Page 6 of 13
7 Exposure Management In the event of an exposure to human blood or other potentially infectious materials, employees of this facility shall do the following (please fill in specifics for this work area): 1. Wash skin and wound exposures with soap and water: Wash for at least 5 minutes with alternate soap and water rinse. 2. Wash eye and mucous membrane exposures in running water for 15 minutes: Eye wash station in the lab 3. Report exposures to the supervisor: Dr. William J. Millard, Room 4-334, HPNP, Phone # or Individuals exposed to human blood or other potentially infectious materials shall go immediately (within one hour) to the following location for treatment: (866) in the Gainesville area Only. This Needle Stick hotline is manned 24 hours a day. You will be directed from there were to go for further treatment In All other Locations: 5. Individuals exposed to human blood or other potentially infectious materials shall go immediately (within one hour) to the following location for treatment. BBP Standard Operating Procedures Page 7 of 13
8 Protective Equipment (by task) Task Aliquotting and pipetting samples Gloves goggles, gowns Personal Protective Equipment Used Vortexing Samples Gloves goggles, gowns Centrifugation of samples Gloves goggles, gowns Aspiration of Samples Gloves goggles, gowns BBP Standard Operating Procedures Page 8 of 13
9 Equipment/Worksite Decontamination Schedule for Neurochemistry Core RIA Facility Facility Pharmacy Building #455 Room P2-41 Location Equipment Schedule Procedure Pipettors End of experimental day Wipe outside and clean inside with 1:100 bleach solution. Gamma Counter End of experimental day Wipe outside with detergent solution Assay Racks End of experiment Soak overnight in 1:10 bleach solution, wash with count-off detergent Pipette tips End of experiment Discard in red biohazard bag Centrifuges End of experiment Wipe outside and clean inside with 1:100 bleach solution. RIA disposables End of experiment Soak overnight in 1:10 bleach solution, discard in radioactive waste. Lab Benches End of experiment Discard bench paper in red biohazard bag. Aspirator End of experiment Soak overnight in 1:10 bleach solution, discard liquid in radioactive waste. BBP Standard Operating Procedures Page 9 of 13
10 Engineering Controls Check for Neurochemistry Core RIA Facility Facility Pharmacy Building #455 Room P2-41 Location Equipment Inspection Dates Action Handwashing facilities Monthly Refill supplies Sharps containers Monthly Empty if necessary Specimen containers Quarterly Check for leaks Regulated waste Monthly Check for leaks, empty if necessary Waste containers Monthly Check for leaks, empty if necessary Mechanical pipettes Monthly Check for contamination, clean if necessary BBP Standard Operating Procedures Page 10 of 13
11 UF Biomedical Waste Plan For Neurochemistry Core RIA Facility Facility Pharmacy Building #455 Room P2-41 Location This plan is based upon the UF Biological Waste Policy. It is designed to ensure compliance with all local, state, and federal regulations and guidelines concerning biological waste, including 29 CFR Part , the OSHA Bloodborne Pathogen rule, and Chapter 64E-16, Florida Administrative Code, Biomedical Waste rule. A. Biomedical Waste In this facility, the following items constitute biomedical waste: Gloves, disposable garments, test tubes, pipettes, needles and syringes used in preparation and analysis of blood or tissue samples from human or animal sources. B. Disposal Containers 1. Red plastic sharps containers shall be used for: needles, syringes, razor blades, scalpel blades 2. Red biohazard bags shall be used for: blood and tissue samples and any container or material in which is contaminated by the blood or tissue samples. 3. Cardboard biohazard boxes shall be used for: gloves, test tubes and pipette tips 4. Regular trash receptacle: paper waste, non-radioactive. BBP Standard Operating Procedures Page 11 of 13
12 C. Personal Protective Equipment The following personal protective equipment shall be worn when handling, packaging and disposing of biomedical waste: Gloves protective eyewear, lab coat (disposable and cloth), mask (as necessary) D. Labeling All packages containing biohazardous/biomedical waste shall be labeled with indelible ink marker (i.e. Sharpie ) as follows: 1. Biohazard bags: Investigators Name Phone Number Room Location 2. Sharps containers: Investigators Name Phone Number Room Location 3. Corrugated boxes (biomedical waste boxes): Investigators Name Phone Number Room Location E. Transport Transport of biomedical waste shall take place as follows: EH&S Waste Management Picks Materials Up and Properly Discards Materials following OSHA Guidelines BBP Standard Operating Procedures Page 12 of 13
13 F. Training regarding biological waste shall take place as follows: Each member of the Neurochemistry Laboratory will be required to undergo annual training by Dr. Millard. This includes an update on all new OSHA and EH&S mandates and viewing of any videotapes that are appropriate. BBP Standard Operating Procedures Page 13 of 13
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