Study of noninvasive Radiofrequency combined with Mesotherapy for face tissue tightening

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1 Raùl Pinto, MD President of SOARME Patricia Frisari, MD Vice President of SOARME Argentine Society of Aesthetic Medicine Study of noninvasive Radiofrequency combined with Mesotherapy for face tissue tightening

2 Fitzpatrick R. et al., Multicenter Study of Noninvasive Radiofrequency for periorbital tissue tightening. Lasers in Surgery and Medicine 2003: 33; Amin SP., Phelps RG., Goldberg DJ., Mesotherapy for facial skin rejuvenation: a clinical, histologic, and electron microscopic evaluation. Dermatol Surg Dec; 32(12): Background Aging Face: Frequent medical consultation Non or less invasive treatments Immediate and lasting effects overtime Walk in/walk out treatments Proposal of cosmetic dermatology Radiofrequency + Mesotherapy

3 Objective of the study To obtain better aesthetic results with two complementary methods Bipolar RF Dermal hydratation before RF Rotational motion of water molecules Less potency needed Immediate contraction of collagen Obtain a uniform distribution of energy Mesotherapy Collagen pool Strong neo-collagen Barini, Cleia, Monopolar Radiofrequency and Bioestimulation with a cluster of aminoacids. III European Congress of Antiaging Medicine EMAA, October 2007.

4 Study design/materials & Methods The study protocol was approved by SOARME review board (a 7-month open-label-study) n=47 (females) Age range: years (mean age: SD=7.8) Medical history Glougau Fitzpatrick wrinkle classification (FWCS ) / Skin types Temperature and Moisture of the skin Photographs / Echographies / Biopsies Self-assessment questionnaire *This Study was not supported by any direct or non-direct funding. It is under the author s own responsibility.

5 Treatment chart

6 Treatment procedure Hydratation + RF: every 21 days (mean number of sessions: 5) Mesotherapy with collagen pool: every 7 days excluding the RF days (mean number of sessions: 10) days Josefina Royo, MD, Multisource, Phase-controlled Radiofrequency for treatment of skin laxity correlation between clinical and Invivo Confocal Microscopy results and real time thermal changes. J Clin Aesthet Dermatol., 2011, January; 4(1): 28-35

7 Treatment procedure Mode Frequency Potency Bipolar/Resistive 1.05 MHz 1 to 65 watts Transductor: 30 mm. (golden electrode) Sterile emulsion coupling Surface temperature ºC Circular and longitudinal multiple passes * Time : 480 seconds Hydratation + RF Hyaluronic acid 0.2% (2 cc) Nappage technique + Radiofrequency * Mesotherapy with collagen pool Glycine....1% Proline. 1% Hydroxyproline. 1% Hyaluronic acid..0.2% 2cc Nappage /Micropapules Harth Y., Lischinsky D., A novel method for real-time skin impedance measurement during radiofrequency skin tightening treatments. J Cosmet Dermatol Mar; 10(1): 24-9.

8 Dermal Hydratation + RF Without treatment

9 Results: Study Population

10 Results: Moisture & Temperature

11 Results: Measurements

12 Results: Subjective evaluation

13 Echographic pattern Increased thickness of dermis (45 of 47 patients) Disappeared pattern of broken fibers at baseline Patient with aging before treatment (age 68) (face) Patient with aging after treatment (age 68) (face) Patient without aging (age 27)

14 Anatomopathology Significant changes in overall appearance Increased thickness of collagen fibers (47/47) Increased thickness of elastic fibers (5/47) Hematoxylin and Eosin Masson trichrome Verhoeff Van Gieson (EVG) Orcein 400x

15 Aesthetic results

16 Aesthetic results

17 Aesthetic results

18 Aesthetic results

19

20 Conclusions Significant clinical, histological and echographic changes were observed with the two complementary methods Although there were different types of skin aging at baseline, the applied treatments tightened the dermal tissue proportionately According to all the patients, the treatment was very satisfactory Follow-up at 18 months (96% of patients) shows maintenance of the aesthetic results achieved

21 References Braccini F., Dohan Ehrenfest DM., Advantages of combined therapies in cosmetic medicine for the treatment of face aging: botulinum toxin, fillers and mesotherapy. Rev Laryngol Otol Rhinol (Bord). 2010; 131(2): French. el-domyati M., el-ammawi TS., Medhat W., Moawad O., Brennan D., Mahoney MG., Uitto J., Radiofrequency facial rejuvenation: evidence-based effect. J Am Acad Dermatol Mar; 64(3): Huth J., Cosmetic Radiofrequency Rev Laryngol Otol Rhinol (Bord). 2010; 131(2): French. Lacarruba F., Tedeschi A., Nardone B., Micali G., Mesotherapy for skin rejuvenation: assessment of the subepidermal low-echogenic band by ultrasound evaluation with cross-sectional B-mode scanning. Dermatol Ther 2008 Nov-Dec.; 21 Suppl. 3: S1-5. Lee M., Facial mesoplasty: mini invasive facial progressive sculpture. Cutaneous restructuration like antiaging technique. Cir. Plást Iberolatinoamericana 2008 Enero-Marzo V 34, N1, Mariggio MA., Cassano A., Vinella A., Vincenti A., Fumarulo R., Lo Muzio L., Maiorano E., Ribatti D., Flavia G., Enhancement of fibroblast proliferation, collagen biosynthesis and production of growth factors as a result of combining sodium hyaluronate and aminoacids Int J Immunopathol Pharmacol 2009 Apr-Jun, 22(2): Naham WK., Rotunda A., Moy RL., Objective changes in brow position, superior palpebral crease, peak angle of the eyebrow and jowl surface area after volumetric radiofrequency treatments to half of the face. Dermatol Surg Jun; 30(6): Willey A., Kilmer S., Newman J., Renton B., Elastometry and clinical results after bipolar radiofrequency treatment skin. Dermatol Surg Jun; 36(6): Zelikson BD., Kist D., Bernstein E., Brown DB., Ksenzenko S., Burns J., Pope K., Histological and ultrastructural evaluation of the effects of a radiofrequency based nonablative dermal remodeling device: a pilot study Arch Dermatol 2004 feb; 140(2):

22 Merci beaucoup! Argentine Society of Aesthetic Medicine

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