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1 850 Lincoln Centre Drive Foster City, CA U.S.A. T F Dear Valued Customer: In an effort to better serve you, Applied Biosystems is revising the look and feel of our Material Safety Data Sheets, (MSDSs). In addition, we will now be supplying MSDSs for materials which are classified as nonhazardous as defined by the Dangerous Substances and Dangerous Preparations Directives, 67/548/EEC and 88/379/EEC respectively, (EU), and the OSHA Hazard Communication Standard, 29 CFR (U.S.). As always, MSDSs will be available in multiple languages. In the coming months, we will be implementing a system whereby you will be able to choose your preferred method for MSDS delivery. Your delivery options are as follows: By E mail By FAX Please take a few minutes to send an to: MSDS_Inquiry_CCRM@appliedbiosystems.com to let us know your preference. Type MSDS Delivery in the subject line and include your preference in the body of the letter along with your full name, Company or Institution, and your preferred address or FAX number as applicable. Please choose only one option. Alternatively, you may print out this letter, circle your preference from the list above, and mail it to the address below. Please include your full name, Company or Institution, and address or FAX number as applicable on this letter. In the meantime, you will continue to receive your document(s) along with your order if it is the first order of the calendar year, or when the MSDS has undergone a major revision. MSDSs are also available on our website. For AB products, please visit: For Ambion products, please visit: If you are a Distributor or Dealer of Applied Biosystems Products: As the last supplier in the chain who provides hazardous substances to a place of work, you have the responsibility to provide a Material Safety Data Sheet (MSDS) and any updated hazard information from our company to your customers. You are required to send the MSDS to your downstream users prior to or with the initial delivery of a hazardous substance. When an MSDS is revised, a copy of the revised MSDS should be sent to the purchaser as soon as it is revised. Should you have any questions, feel free to contact us via at: MSDS_Inquiry_CCRM@appliedbiosystems.com or call (USA) Thank you for your continued business. Vergine Furniss Global Chemical Compliance and Risk Management Applied Biosystems 850 Lincoln Centre Drive Foster City, CA x5635 (USA) Revision Date: 5-Jan-07 Version 1.0

2 MATERIAL SAFETY DATA SHEET 1 PRODUCT AND COMPANY IDENTIFICATION Product Name: ACRIDINE ORANGE STAIN Product No.: F8555G Trade Name(s): LCM Staining Kit, AM1935, 1935, 8555G Manufacturer Name: Ambion Inc Woodward Street Austin, TX, USA Phone: (512) Emergency Telephone: (NORTH AMERICA); (INTERNATIONAL, REVERSE CHARGES) Intended Use: For Research Use Only, Not for Use in Diagnostic Procedures 2 HAZARDS IDENTIFICATION Emergency Overview Physical State: Liquid Color: No data available. Odor: No data available. WARNING! Causes eye irritation. Flammable liquid and vapor. Potential Health Effects Inhalation: Vapors may cause drowsiness and dizziness. Eye Contact: Causes eye irritation. Exposed individuals may experience eye tearing, redness, and discomfort. Skin Contact: Prolonged or repeated contact may dry skin and cause irritation. Ingestion: Causes digestive tract burns. May cause burns in mucous membranes, throat, esophagus and stomach. NA ENGLISH MSDS 1 / 8

3 Chronic Health Effects: Repeated exposure may cause skin dryness or cracking. Target Organ(s): Eye Skin Potential Physical / Chemical Effects: The product is flammable, and heating may generate vapors which may form explosive vapor/air mixtures. OSHA Regulatory Status: This product is hazardous according to OSHA 29CFR Environment: The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment. The product contains a substance which has a photochemical ozone creation potential. 3 COMPOSITION / INFORMATION ON INGREDIENTS General Information: The product contains: Organic solvent. Chemical Name CAS-No. Concentration* Ethanol > 60% * All concentrations are percent by weight unless ingredient is a gas. Gas concentrations are in percent by volume. This chemical is hazardous according to OSHA/WHMIS criteria. 4 FIRST AID MEASURES General: Thermal burns: Flush with water immediately. While flushing, remove clothes which do not adhere to affected area. Call an ambulance. Continue flushing during transport to hospital. Inhalation: Move injured person into fresh air and keep person calm under observation. Get medical attention if any discomfort continues. Eye Contact: Immediately flush with plenty of water for up to 15 minutes. Remove any contact lenses and open eyes wide apart. Get medical attention promptly if symptoms occur after washing. Skin Contact: Remove contaminated clothes and rinse skin thoroughly with water for at least 15 minutes. Get medical attention promptly if symptoms occur after washing. Ingestion: Rinse mouth thoroughly with water and give large amounts of milk or water to people not unconscious. Get medical attention if any discomfort continues. 5 FIRE-FIGHTING MEASURES Extinguishing Media: Extinguish with alcohol-resistant foam, carbon dioxide or dry powder. Unsuitable Extinguishing Media: Not applicable. Special Fire Fighting Procedures: Use standard firefighting procedures and consider the hazards of other involved materials. Cool containers exposed to heat with water spray and remove container, if no risk is involved. Unusual Fire & Explosion Hazards: Heating will generate vapors which may form explosive vapor/air NA ENGLISH MSDS 2 / 8

4 mixtures. Hazardous Combustion Products: Carbon Dioxide, Carbon Monoxide Protective Measures: Selection of respiratory protection for fire fighting: follow the general fire precautions indicated in the workplace. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Flammability Class: NFPA Rating Fire = 3. 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Extinguish all ignition sources. Avoid sparks, flames, heat and smoking. Ventilate. Avoid inhalation of vapors and contact with skin and eyes. Wear suitable protective clothing. See Section 8 of the MSDS for Personal Protective Equipment. Spill Cleanup Methods: Absorb spillage with non-combustible, absorbent material. For waste disposal, see section 13 of the MSDS. Notification Procedures: Inform authorities if large amounts are involved. 7 HANDLING AND STORAGE Handling: Local exhaust is recommended. Use explosion proof electric equipment. Ground container and transfer equipment to eliminate static electric sparks. The product is flammable, and heating may generate vapors which may form explosive vapor/air mixtures. Do not smoke and do not spray near an open flame or other sources of ignition. Avoid inhalation of vapors and contact with skin and eyes. Wear approved safety goggles. Wear protective gloves and appropriate clothing to prevent skin contact. Observe good industrial hygiene practices. Storage: Follow rules for flammable liquids. Keep away from heat, sparks and open flame. Store in closed original container in a dry place. Keep away from food, drink and animal feeding stuffs. 8 EXPOSURE CONTROLS / PERSONAL PROTECTION Exposure Limits: Chemical Name Source Type Exposure Limits Notes Ethanol ACGIH TWA 1000 ppm Ethanol NIOSH Guide IDLH 3300 ppm Ethanol US. OSHA Table Z-1 TWA 1000 ppm 1900 mg/m³ Consult Canadian Provincial Regulations and/or Mexican Regulations on exposure limits, if applicable. Engineering Controls: Provide adequate ventilation. Observe Occupational Exposure Limits and minimize the risk of inhalation of vapors. Provide easy access to water supply or an emergency shower. Respiratory Protection: If engineering controls do not maintain airborne concentrations below recommended exposure limits (where applicable) or to an acceptable level (in countries where exposure limits have not been established), an approved respirator must be worn. In the United States of America, if respirators are used, a program should be instituted to assure compliance with OSHA Standard 63 FR NA ENGLISH MSDS 3 / 8

5 1152, January 8, Seek advice from local supervisor. Eye Protection: Wear approved safety goggles. Hand Protection: Wear protective gloves. Be aware that the liquid may penetrate the gloves. Frequent change is advisable. Suitable gloves can be recommended by the glove supplier. Skin Protection: Wear appropriate clothing to prevent repeated or prolonged skin contact. Apron and long sleeves are recommended. Hygiene Measures: Always observe good personal hygiene measures, such as washing after handling the material and before eating, drinking, and/or smoking. Routinely wash work clothing and protective equipment to remove contaminants. 9 PHYSICAL AND CHEMICAL PROPERTIES Color: No data available. Odor: No data available. Odor Threshold: No data available. Physical State: Liquid ph: No data available Melting Point: No data available. Freezing Point: No data available. Boiling Point: No data available. Flash Point: 13 C (55 F) (Ethanol) Evaporation Rate: No data available. Flammability Limit - Upper (%): No data available. Flammability Limit - Lower (%): No data available. Vapor Pressure: No data available. Vapor Density (Air=1): No data available. Specific Gravity: No data available. Solubility in Water: Soluble Solubility (Other): No data available. Partition Coefficient (n-octanol/water): No data available. Autoignition Temperature: No data available. Decomposition Temperature: No data available. Viscosity: No data available. 10 STABILITY AND REACTIVITY Stability: This product is stable under expected conditions of use. Conditions to Avoid: SparksHeat, sparks, flames. Ignition sources. Incompatible Materials: Strong oxidizing agents. Alkali metals. Fluorine. Halogens. Nitric acid. Potassium permanganate. Hazardous Decomposition Products: At Elevated Temperatures: Carbon Dioxide, Carbon Monoxide NA ENGLISH MSDS 4 / 8

6 11 TOXICOLOGICAL INFORMATION Specified Substance(s) Acute Toxicity: Component Chemical Name Ethanol Ethanol Test Results Inhalation LC50 (10 hour(s), Rat): ppm (m) Oral LD50 (Rat): 7060 mg/kg Listed Carcinogens: Chemical Name IARC NTP OSHA ACGIH Ethanol Not Listed Not Listed Not Listed A4 IARC: 1 = Carcinogenic to Humans; 2A = Probably Carcinogenic to Humans; 2B = Possibly Carcinogenic to Humans; 3 = Not classifiable as to carcinogenicity to humans; 4 = Probably not carcinogenic to humans; Not listed = Not evaluated by IARC. ACGIH: A1 = Confirmed Human Carcinogen; A2 = Suspected Human Carcinogen; A3 = Confirmed Animal Carcinogen; A4 = Not classifiable as a human carcinogen; A5 = Not suspected to be a human carcinogen; Not listed = Not evaluated by ACGIH. Product Information Acute Toxicity: Causes eye irritation. Prolonged or repeated contact may dry skin and cause irritation. Chronic Toxicity: Repeated exposure may cause skin dryness or cracking. Prolonged or repeated exposure may cause: High concentrations: May cause central nervous system effects. 12 ECOLOGICAL INFORMATION Ecotoxicity: The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment. Mobility: The product is water soluble and may spread in water systems. The product contains organic solvents which will evaporate easily from all surfaces. Persistence and Degradability: No data available. Bioaccumulation Potential: No data available. Other Adverse Effects: The product contains a substance which has a photochemical ozone creation potential. 13 DISPOSAL CONSIDERATIONS General Information: Dispose of waste and residues in accordance with local authority requirements. Disposal Methods: No specific disposal method required. Container: Since emptied containers retain product residue, follow label warnings even after container is emptied. 14 TRANSPORT INFORMATION NA ENGLISH MSDS 5 / 8

7 DOT UN No.: UN1170 Proper Shipping Name: Ethanol solutions Class: 3 Packing Group: II Label(s): 3 TDG UN No.: UN1170 Proper Shipping Name: ETHANOL SOLUTION Class: 3 Packing Group: II IATA UN No.: UN1170 Proper Shipping Name: Ethanol solution Class: 3 Packing Group: II Label(s): Flamm. liquid IMDG UN No.: UN1170 Proper Shipping Name: ETHANOL SOLUTION Class: 3 Packing Group: II EmS No.: F-E, S-D 15 REGULATORY INFORMATION Canadian Controlled Products Regulations: This product has been classified according to the hazard criteria of the Canadian Controlled Products Regulations, Section 33, and the MSDS contains all required information. WHMIS Classification: B2, D2B Mexican Dangerous Statement: This product is dangerous according to Mexican regulations. Inventory Status: US TSCA inventory Status: All the components of this substance are listed on or are exempt from the inventory. For purposes of 40 CFR , this product is for Research and Development (R&D) Use Only. US Regulations CERCLA Hazardous Substance List (40 CFR 302.4): Chemical Name Ethanol RQ 100 lbs SARA Title III Section 302 Extremely Hazardous Substances (40 CFR 355, Appendix A): Not regulated. NA ENGLISH MSDS 6 / 8

8 Section 311/312 (40 CFR 370): X Acute (Immediate) Chronic (Delayed) X Fire Reactive Pressure Generating Section 313 Toxic Release Inventory (40 CFR 372): Not regulated. Clean Air Act (CAA) Section 112(r) Accidental Release Prevention (40 CFR ): Not regulated. Clean Water Act Section 311 Hazardous Substances (40 CFR 117.3): Not regulated. Drug Enforcement Act: Not regulated. TSCA: TSCA Section 4(a) Final Test Rules & Testing Consent Orders: Not regulated. TSCA Section 5(a)(2) Final Significant New Use Rules (SNURs) (40CFR 721, Subpt. E): Not regulated. TSCA Section 5(e) PMN-Substance Consent Orders: Not regulated. TSCA Section 12(b) Export Notification (40 CFR 707, Subpt. D): Not regulated. State Regulations California Safe Drinking Water and Toxic Enforcement Act of 1986 (Proposition 65): Not regulated. Massachusetts Right-To-Know List: Ethanol Michigan Critical Materials List (Michigan Natural Resources and Environmental Protection Act (Act. 451 of 1994)): Not regulated. Minnesota Hazardous Substances List: Ethanol New Jersey Right-To-Know List: Ethanol Pennsylvania Right-To-Know List: Ethanol Rhode Island Right-To-Know List: Ethanol 16 OTHER INFORMATION HAZARD RATINGS Health Hazard Fire Hazard Instability Special Hazard NFPA Hazard rating: 0 - Minimal; 1 - Slight; 2 - Moderate; 3 - Serious; 4 - Severe NFPA Label colored diamond code: Blue - Health; Red - Flammability; Yellow - Instability; White - Special Hazards Health Hazard Flammability Physical Hazard Personal Protection NA ENGLISH MSDS 7 / 8

9 HMIS B Hazard rating: 0 - Minimal; 1 - Slight; 2 - Moderate; 3 - Serious; 4 - Severe Personal Protection codes: B - Safety Glasses, Gloves HMIS Label colored bar code: Blue - Health; Red - Flammability; Orange - Physical Hazards; White - Special MSDS PN: Disclaimer: This MSDS has been prepared using information from sources considered technically reliable. It should not be relied upon as a product specification. The company makes no warranty of any kind, express or implied, concerning the safe use of this material in your process or in combination with other chemical substances. Every user of the product is responsible for evaluating its own processes and conditions of use and selecting appropriate protective measures for employee exposure. NA ENGLISH MSDS 8 / 8

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