Levan. A Natural Anti-Aging Humectant

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1 Levan A Natural Anti-Aging Humectant

2 What is Levan? Levan is a naturally occurring bio-polymer a polyfructose. Levan molecules are formed from sucrose by an enzymatic process. Typically structured as β-2,6 carbon atom bonds, they are in fact edible, prebiotic fibers, appearing as a whitish, slightly viscous solution. Efficacy & uniqueness of these fibers are derived from their structure & MW distribution. Combining low & high MW, Levan performs as an outstanding film-forming Humectant. Levan 2, 6 bonds Inulin 1,2 bonds

3 Levan Production process & Safety Production process: enzymatic conversion of sugar. Conducted under strict GMP conditions. Enzyme: Non-GMO, Infoxgen compliance; certificated European enzyme manufacturer; enzymatic process doesn't require growth medium; culture is not involved in the production of levan. Contamination control: Pasteurization at the end of the process. Preservation: Potassium Sorbate. INCI name / 28-30% Levan: Levan/Fructose/Glucose/Sucrose (and) Citrate Buffer Composition: Levan fiber, sugars, buffering agent, sorbate. Non-Irritation / Dermatology Tested Certified: Checked up to 10% conc. in body cream formulations/ as ingredient in direct contact with skin.

4 Gan Shmuel s Levan s researched advantages in topical skin-care: Water retention Film forming Moisturizing Cell proliferation Anti-inflammatory response Pro-Collagen synthesis activator Comparable TEWL prevention efficacy to HA Comparable Hydrating (Corneometer) efficacy to HA Compound stable Dermatology Tested

5 Clinical Research : Levan Efficacy vs hyaluronic acid Performing Laboratory: The Israeli Institute for Skin Research Principal Investigator: Prof. Sarah Brenner, Dermatologist Sponsor: Gan Shmuel Foods Ltd. 1 Objective: To compare between the efficacy of Levan solution (28-30%) 1.6%, Hyaluronic Acid (HA) 0.2% and a Compound of Levan solution 0.8% + Hyaluronic Acid (HA) 0.1% in Anti Wrinkle Treatment Test materials: 1. Levan solution (28-30%) 1.6% 2 Research groups: 2. Levan solution 0.8% + HA 0.1% Body cream Group-A (Levan 0.8% + HA 0.1%) 3. HA 0.2% Body cream Group-B (Levan 1.6%) Body cream Group-C (HA 0.2%) 23 participants

6 Clinical Research Clinical assessment The following examinations will be performed at baseline (T0), before using the materials, And at the end of the test (T1), after four weeks of using the materials: Visual skin assessment by the investigator in a scale of 0 5, with reference to wrinkles appearance, wrinkles surface area and depth. Measurements: Hydration level by Corneometer, Courage & Khazaka, CK. constant pressure on skin/ hydration measurements. TEWL (the ability not to harm the skin barrier) by Tewameter TM 300, Courage & Khazaka, CK. Measurement will be recorded after stabilization of the curve of the results in real time. Photography Color photography by Visioface 1000 D, Courage & Khazaka, CK from the front and the sides of the face Wrinkle analysis Selected wrinkles area will be analyzed by Visioface 1000 D, Courage & Khazaka, CK.

7 Clinical Research results: Corneometer Body cream Group-A (Levan 0.8% + HA 0.1%) vs Group-B (Levan 1.6%) Hydration level (Corneometer) Levan vs. HA half-dosage+ Levan T0 T1 A B Figure 1: Skin hydration product A vs. product B The hydration level of the side that was treated with product A increased from 63.0 to 65 The hydration level of the side that was treated with product B increased from 62.4 to 65.9

8 Clinical Research results: TEWL Body cream Group-A (Levan 0.8% + HA 0.1%) vs Group-B (Levan 1.6%) TEWL level Levan vs. HA at half-dosage + Levan T0 T1 A B Figure 3: TEWL level product A vs. product B The TEWL level of the side that was treated with product A decreased from 6.4 g/hm 2 to 6.2 g/hm 2 The TEWL level of the side that was treated with product B decreased from 6.4 g/hm 2 to 6.2 g/hm 2

9 Clinical Research results: Wrinkle assessment by researcher Body cream Body cream Group-C (HA 0.2%) vs Group-B (Levan 1.6%) Wrinkle assessment by researcher Levan (alone) vs. HA T0 T1 C B Figure 6: Wrinkle assessment product C vs product B The average wrinkle assessment of the side that was applied with product B had a statistically significant decrease, from 4.5 to 4.0 after 4 weeks of application (p<0.05).

10 Clinical Research results: wrinkle assessment by volunteers Body cream Group-C (HA 0.2%) vs body cream Group-B (Levan 1.6%) Wrinkle improvement according to volunteers Levan (alone) vs. HA C B Wrinkle improvement Figure 8: Volunteers assessment of wrinkle improvement C vs. B Product B received the score 4.6 out of 10 and product C received the score 4.1 (in a 0-10 scale).

11 Clinical Research results/products assessment by the Volunteers Body cream Group-A (Levan 1.6%) vs Body cream Group-B (HA+Levan ) double-blind Product assessment by the volunteers/ Levan vs. HA half-dosage + Levan After 4 weeks of using the product the skin is more radiant After 4 weeks of using the product the skin is more pleasant to touch After 4 weeks of using the product the skin Significantly looks smoother No tingling or redness after application The skin is firmer The skin area around the eye is less wrinkle A B

12 Clinical Research results/products assessment by the Volunteers Body cream Group-B (Levan 1.6%) vs Body cream Group-C (HA 0.2%) double-blind Product assessment by the volunteers/ Levan vs. HA After 4 weeks of using the product the skin is more radiant After 4 weeks of using the product the skin is more pleasant to touch After 4 weeks of using the product the skin Significantly looks smoother No tingling or redness after application The skin is firer The Skin area around the eye is less wrinkled B C

13 Clinical Research results/purchase intentions Body cream Group-B (Levan 1.6%) vs Body cream Group-C (HA 0.2%) vs Group-A (Levan 0.8% + HA 0.1%) 30% Purchase intention Product A Levan + HA Purchase intention Product C - HA 20% 20% Purchase intention Product B Levan 70% 80% 80% YES NO double-blind assessment by volunteers Levan alone vs. HA half-dosage + Levan vs. HA alone

14 Potential Levan applications in skin-care Anti-aging, moisturizing creams/gels/lotions/ointments/serums Eye pads Nurturing masks UV-damage cell repair products HA-based products Rinse-off feminine wash Body firming-shaping creams/gels.

15 Levan Natural & Unique Effective & Stable Raw material source: Sugar Edible, prebiotic fiber Enzyme: Non-GMO (+ Infoxagen statement) Traceable supply chain; GMP manufacturing Contains both low & high MW at specified ratio: anti-aging efficacy Dermatology Tested (ingredient / cream) Clinically researched: Equal or Better than HA {TEWL, Hydration, wrinkle improvement, volunteers feedback; ISR, } ph 5.5; White color; odorless; INCI registered; CAS Stable in gel/cream formulations (France, August-2017) Cost- Saving When replacing HA in formulation When blended with HA (reduced dosage) in formulation

16 About the manufacturer Gan-Shmuel Foods ltd With over 80 years of manufacturing experience, Gan Shmuel Foods ( is a dynamic company with a distinguished heritage of innovation and entrepreneurship. Merging two of Israel's largest citrus and fruit products processors- Gan Shmuel Foods, and Ganir - the company s core business is quality, tailor-made fruit-based solutions for the beverage industry. The company is Israel s largest exporter from the Food & beverages sector. It markets products in Europe, South America, South East Asia, Turkey & the Middle East. Its business consists of B2B products, as well as own-brand selections under the high-end Primor brand. Gan-Shmuel Foods is a publicly traded company whose values, work ethic and innovative spirit are inspired by its Kibbutz origins as a collective enterprise. It strives to maintain a sustainable business ecosystem which improves the lives of both its customers, and employees. Levan is one of several in-house Research & Development projects, which utilize natural raw materials, already in use by the company for its beverage business - for new, added-value applications in other product segments.

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