Hyabest (S) LF-P. An Ideal Hyaluronic Acid for Skin-beauty Supplements

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1 Hyabest (S) LF-P An Ideal Hyaluronic Acid for Skin-beauty Supplements Since 1983, the Kewpie Corporation of Japan has been producing a wide range of Hyaluronic Acid products, along with its sodium salt. Through these efforts it has become one of the world s leading producers, based on the products high degree of refinement and scientifically proven quality. Food grade Hyabest (S) LF-P, developed specially for products that satisfy the beauty from within concept, has become one of Kewpie s most popular brands. Kewpie recently obtained new data showing that, taken orally, Hyabest (S) LF-P suppresses skin damage due to ultraviolet radiation. In June, Kewpie released this data to the 2013 International Society for Hyaluronan Sciences conference, held in Oklahoma City. The data, involving hairless mice, shows that daily oral administration of an equivalent of 200 mg of hyaluronic acid for six weeks significantly suppressed the decrease of skin moisture volume and resultant wrinkle formation associated with ultraviolet radiation (Hyabest (S) LF-P vs. UV irradiation contagion on skin). The publication of this data follows on Kewpie s previously conducted testing, which showed a significant increase in skin moisture, along with other improvements, as a result of six weeks of daily oral intake of 120 mg of hyaluronic acid (Hyabest (S) LF-P skin improvement effect), (Journal of New Remedies & Clinics Vol.58 No.8; Aug.10, 2009). Health food holds about a USD $6.85 billion share of the Japanese market. Sixteen percent of this market, about USD $1.12 billion, comprises beauty-related foods, of which 24%, equal to about USD $269 million, contain hyaluronic acid. 1 With the popularity of Kewpie s marketing programs, solidly supported by carefully researched scientific evidence, foods and beauty supplements containing the company s hyaluronic acid are currently enjoying unprecedented popularity in Japan. 1. H B Foods marketing handbook 2013, Fuji-Keizai Group

2 Hyabest(S) LF-P v.s. UV irradiation contagion on skin Animals: male hairless mice HOS:HR-1 (4 weeks) UV irradiation source: lump GL20SE (SANKYO DENKI) Orally administration of HA or control 200mg/kg BW (week) Randomly divided Total irradiation: 2.8J/cm 2 UV(-) control group (n = 6) photograph UV(+) control group (n = 6) UV(+) HA group (n = 5)

3 1. Skin moisture change by UV irradiation CM unit UV(-) control UV(+) control UV(+) HA20 万 * 群分け時 0week 1week 2week 3week 4week 5week 6week Hyabest(S)LF-P suppressed skin moisture decrease by UV irradiation

4 2. Wrinkle appearance UV(-)control UV(+)control UV(+) HA

5 Hyabest (S) LF-P skin improvement effect 1 Increased amount of skin moisture (a.u.) Test food : Hyabest (S) LF-P supplement (120mg /day) Placebo : Crystalline cellulose Test subjects : Japanese female from 35age to 60age HA group N=20, Placebo group N=22 Test methods : Double-blind test (parallel 2groups) Measured item : Measurements of cheek skin moisture Measuring device : CORNEOMETER CM825 Hyabest (S) LF-P 120mg/day PLACEBO Before intake 3 weeks 6 weeks 2 weeks after intake ends Skin moisture increase by oral administration of the HA was confirmed J. New Rem. & Clin. Vol.58:1469~1481, 2009

6 Hyabest (S) LF-P skin improvement effect 2 Measured item : Change of skin texture replica over time Measuring device : ASA-03R Average depth of skin (μm) Test product HA group (N=20) Placebo group (N=22) Before intake After 3 weeks of intake After 6 weeks of intake 38.2± ± ± ± ± ±3.8 Average value ± standard deviation *:p<0.05 vs. before intake, Dunnett test * * Before intake (right cheek) HA group 6 weeks after intake Skin texture improvement by oral administration of the HA was confirmed J. New Rem. & Clin. Vol.58:1469~1481, 2009

7 Hyabest (S) LF-P skin improvement effect 3 6 Measuring method : Questionaire survey (N=20) Judging criteria : Scoring 1~5 (the score -the smaller, the better ) 5 4 Score 3 2 * * * * * * つや Luster はり Firmness くすみ Color Dullness シミ Age spot シワ Wrinkles 1 0 Before 1 intake 3 weeks 2 after 6 weeks 3 after intakeiintakeb Average value±standard deviation *:p<0.01 vs. before intake Wilcoxon s signed rank sum test Varoius skin condition scores showed improvements by oral administration of the HA J. New Rem. & Clin. Vol.58:1469~1481, 2009

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