Biosafety Self-Audit Checklist

Transcription

1 Biosafety Self-Audit Checklist Principal Investigator: Biosafety Certificate #: Location: Audited By: Date: Posting: Dalhousie University Hazard Identification poster with biohazard symbol posted on lab door Containment Level of the lab posted on lab door Emergency contact information posted Biohazard signs and/or symbols posted on storage and use areas Biohazard symbol posted to potentially contaminated equipment Biohazardous waste stored and treated appropriately Refrigerators labeled Not For Storage of Food for Human Consumption Dalhousie University Biosafety Manual available PHAC Canadian Biosafety Standards and Guidelines available (online acceptable)

2 Facility: General housekeeping satisfactory No food or drink consumption permitted in the laboratory Containment cabinet operational and in good repair Containment cabinet certification current Designated clean areas present Hand washing facility available Eye wash station available Safety shower available Work surfaces cleanable and impervious Chair cover fabric readily decontaminated Autoclave working with calibration and log maintained Autoclave registered with EH&S Monthly biological indicator testing done on autoclave Centrifuge in good working order

3 Aerosol producing equipment contained in devices that exhaust through HEPA filters Mechanical pipetting devices available Biosafety spill kit available Sharps containers available Laboratory doors are closed at all times Standard Operating Procedure (SOP) manual available and procedures followed A local risk assessment has been performed for procedures and processes that pose a risk, and an SOP has been generated to mitigate this risk Personnel: Lab coats worn Appropriate disposable gloves worn Eye protection available and worn Long hair tied back or restrained Suitable footwear with closed toes and heels worn by personnel Lab coats and gloves removed before exiting designated biohazard area Proper work procedures followed to avoid contamination and aerosol creation Mouth pipetting forbidden The use of needles, syringes and other sharp objects limited to avoid needle stick injury

4 Personnel know and follow proper glove removal technique Personnel know and follow proper hand washing technique Workers are registered with the EH&S office, and posted approved worker list is up to date Workers have read the Dalhousie University Biosafety Manual Workers been trained in proper use of containment cabinets Workers been trained in the safe use of autoclaves Workers been trained in effective decontamination techniques for agent used Biosecurity: Laboratory locked when room is unoccupied Biological agents secure against unauthorized removal Inventory records up-to-date to detect any loss or theft All keys issued are accounted for Access to the laboratory is limited to authorized personnel

5 Disinfection, Decontamination & Waste Disposal Work surfaces decontaminated at least daily and immediately after a spill Decontamination techniques effective against agents used Waste disposal as per Dalhousie University s Policy for Management of Biological Waste, which is available for reference in the University s Biosafety Manual. Emergency Response: Emergency spill procedures posted All personnel are aware of the requirement to immediately report accidents & spills that result in exposure to the P.I. All personnel are aware of the requirement to report accidents & incidents to the EH&S office, and know where to find the Incident Report Form During this past audit period, the P.I. has reviewed emergency response procedures with personnel During this past audit period, the P.I. has reviewed the location of spill kits, eye wash stations, and safety showers with personnel

6 This laboratory is classified as: Level 1 Level 2 Level 3 During this past audit period the following biological agents were used: Bacterial rdna Viral Toxins Fungal Animals Parasitic Human/primate blood Rickettsial Human body fluid, cells Prions Other potential infectious material, specify Office Use Only: Verification: Date: : Signature: