Mare's milk in cosmetics: preliminary tests

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Mare's milk in cosmetics: preliminary tests Dijana Gavrić 1, Mateja Kovačič 1, Metka Pliberšek 1, Petra Keršmanc 1, Katja Žmitek 1, 2 Klemen Potočnik 3 Klemen.Potocnik@bf.uni-lj.si University of Ljubljana Biotechnical Faculty

Mare's milk in cosmetic topical use Key ingridients: PUFA, albumin, globulins and peptides, free amino acids, a high content of carotene, vitamins, minerals and other bioactive components. Baths in mare's/asse's milk Queen Cleopatra Empress Elisabeth of Austria Sisi Published possitive effects by: inflammatory skin diseases, neurodermatitis, eczema, psoriasis, acne, etc.

Aim of the work Preservation of local breeds With production Equine production is a highly variable Races - Sports Leisure EAAT Tourism Meat & Milk (limited niche in E. & NA)

Idea developing Milk production with local breeds technology Registration of mare's milk production unit Selling mare's milk Not so easy Connected to: research organizations cosmetics Businesses cosmetics, machinery Use mare s milk as raw material for cosmetics

Material 3 ladies different ages with dry skin Tonic Water Mare s milk Cream Placebo Placebo + 4% FD MM Twice a day Two months

1. Clean Phase CLEANSING FOAM 2. Mare s milk as tonic 3. MM creme 1. Clean Phase CLEANSING FOAM 2. Water as tonic 3. Placebo creme

Methods Apparatus Multi Skin Test Center MC 750 & Visioface Quick Measurements skin moisture, skin elasticity, skin ph, the depth of wrinkles, fine pores, large pores and dark spots On the face

Cream differences - viscosity 51,000 mpas 36,000 mpas

Results 1 st model - forehead 70 60 50 40 30 20 10 30.3.2012 leva stran 23.4.2012 leva stran 28.5.2012 leva stran 30.3.2012 desna stran 23.4.2012 desna stran 28.5.2012 desna stran 0 hydration Vlažnost ph Elastičnost elasiticity

Results 1 st model - cheek 70 60 50 40 30 20 10 30.3.2012 leva stran 23.4.2012 leva stran 28.5.2012 leva stran 30.3.2012 desna stran 23.4.2012 desna stran 28.5.2012 desna stran 0 hydration ph elasiticity Vlažnost ph Elastičnost

80 70 Results 3 rd model - forehead Chicken pox 60 50 40 30 20 10 30.3.2012 leva stran 23.4.2012 leva stran 28.5.2012 leva stran 30.3.2012 desna stran 23.4.2012 desna stran 28.5.2012 desna stran 0 Vlažnost ph Elastičnost hydration ph elasiticity

Results 3 rd model - cheek Chicken pox hydration ph elasiticity

Results 1 st model - forehead 5 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Gube wrinkles Pore fine Pore large Pigmentni pores F pores L spots madeži 30.3.2012 leva stran 23.4.2012 leva stran 28.5.2012 leva stran 30.3.2012 desna stran 23.4.2012 desna stran 28.5.2012 desna stran

6 Results 1 st model - cheek 5 4 3 2 1 30.3.2012 leva stran 23.4.2012 leva stran 28.5.2012 leva stran 30.3.2012 desna stran 23.4.2012 desna stran 28.5.2012 desna stran 0 Gube Pore fine Pore large Pigmentni wrinkles pores F pores L spots madeži

Before-after comparison

Conclusions Results support the positive effect of mare s milk for cosmetic purposes Use of cosmetics care with mare s milk in comparison to placebo cosmetics increasing humidity and elasticity reducing wrinkles and pores reducing dark spots (visibility) Use of mare s milk in cosmetics could improve breeding economics of horse breeding Local breeds could be us as brand mark

Future work Certificate more products Clinical studies on cosmetics & dermatology? Second diploma defence will be on 1 st of September Spread know how abroad Croatia as an example donkey breeding organisation Use our certificated product Try to established market (with no investment) Use our technology and knowhow Own production

Thank You for your attention!