THE CINCO DE MAYO STUDY Temporal Changes in the Facial Skin Microbiome - A One-Year Longitudinal Study in Normal Healthy Men and Women Kausar Malik Amway Corporation, Ada, Michigan October 29, 2018
WORLD CLASS BRANDS - Four Business Categories BEAUTY / PERSONAL CARE HOME ENERGY SPORT NUTRITION 2
PRESENTATION OUTLINE - Background and Objectives - Study Design and Methods - Findings - Conclusion 3
BACKGROUND - Predominant Microflora Phylum Genus Skin Physiology Actinobacteria Propionibacteria Sebaceous Actinobacteria Firmicutes RESIDENT MICROFLORA Corynebacteria Staphylococci Moist Proteobacteria Pseudomonas Dry Microbial Diversity and Stability Low Microbial Diversity High Stability High Microbial Diversity Low stability Depending on the skin conditions (sebaceous, moist or dry) - Different bacterial species harbor different parts of a human body 4
HUMAN MICROBIOTA FUNCTION Skin Commensals act as Immunomodulators Educate Prime Adaptive Immunity Tune local cytokine production Educate adaptive immunity Influence regulatory T-cells Enhance host innate immunity Increase AMP production Reduce inflammation after injury Strengthen epidermal barrier Directly inhibit pathogen growth Competition -Occupy space and nutrients Inhibition- Produce AMPs Antagonism - Prevent S. aureus biofilm formation Major mechanisms of action of probiotics* *Probiotic Mechanisms of Action - Miriam Bermudez-Brito et al. Ann Nutr Metab 2012; 61:160 174 5
BACKGROUND Variability in community composition among healthy individuals exceeds the variability within individuals over time: Inter-individual variability is higher than Intra-Individual Despite the skin's exposure to the external environment, Microbial communities are largely stable over time 6
MICROBIOME STUDY OBECTIVES 1. Measure the Stability of Skin Microbiome over several years in a large population of healthy adults. 2. Measure the association of Skin Microbiome Diversity and Composition with facial skin condition using biophysical and imaging methods. 3. Determine if the skin microbiome composition co-varies with important signs of skin health and beauty to develop microbiome-based treatment interventions. Goal Target Scientifically Sound Technology in Amway Products 7
CINCO DE MAYO - LONGITUDINAL COHORT Cinco de Mayo Cohort ~150 Amway employee volunteers Skin measurements annually in May since 2014 Apples vs apples: Same staff, instruments, etc.. Added skin microbiome in 2017 and 2018 Note: Most women used make-up between two sampling points for microbiome 8
CINCO DE MAYO LONGITUDINAL STUDY Conducted in 2017 and 2018 - On 150 Normal Healthy Men and Women Measured - Biophysical Skin Properties - - Barrier Function, Skin Surface ph and Elasticity, Visible Facial Features - - Wrinkles, Hyperpigmentation, Porphyrins, Changes in Skin Health Microbiome - - Changes in Skin Microbiome Composition and Diversity The study is designed to collect data over a 10-year period to identify the visual signs of skin aging, and wrinkles and shift in natural skin microflora composition. 9
2017 AND 2018 COHORTS Longitudinal Study Subjects May, 2017 May, 2018 n=155 n=150 60 2017 Age Distribution Number of Subjects 50 40 30 20 10 n=79 n=88 n=58 n=67 n=86 n=79 n=58 n=64 0 20s 30s 40s 50s 60s Decade 90% Caucasian Age 26-61 90% Caucasian Age 27-62 90% at Age = 30s - 50s 10
STUDY FLOW Individual Consent Microbiome ph Face Cleansing Height/Weight Elasticity Imaging Questionnaire Equilibrate 20 Barrier Function Exit Microbiome Samples Requirement Dirty Face No face washing No Make Up 11
FACIAL MEASUREMENTS - METHODS Facial Swabbing Skin Microbiome Forehead Left+Right Cheeks Buffer-wetted Sterile Swabs 16S Amplicon Sequencing V4 and V1-V3 Regions Facial Features Left/Right Cheeks and Forehead Standardized Imaging and Image Analysis Canfield Scientific VISIA CR and VAESTRO Region of Interest (ROI) Exact Same 12
FACIAL MEASUREMENTS Skin Feature Detection and Quantitation Standard 1 for Wrinkles and Pores Multiple Lighting Modalities Wrinkles Visible Spots Brown Spots Red Features Porphyrins Pores Ghost Repositioning for 2017 and 2018 image analysis Locked image location for each subject to get the image at the same location each year 13
FACIAL MEASUREMENTS Contd. Skin Elasticity Left Cheek Suction Method C&K Cutometer N=3 per site. Stratum Corneum Barrier Function Left Cheek and Forehead Transepidermal Water Loss Delfin Vapometer N=3 per site. Surface ph (2018 only) Left Cheek ph meter - (Mettler Toledo flat probe) N=3 per site 14
RESULTS DATA ANALYSIS Facial Microbiome Composition and Diversity 15
DATA -FACIAL COMMUNITY COMPOSITION Cheeks and Forehead Microbiome Composition using V4 and V1-V3 Primers V4 V4 2017 vs. 2018 V4 vs. V1-V3 Composition Relatively moderate differentiation between the cheek and forehead microbiomes. V1-V3 V1-V3 Insignificant differentiation between 2017 and 2018. No difference between V4 vs. V1-V3 Primers 16
CHANGE IN ALPHA DIVERSITY OVER 1 YEAR Alpha diversity Diversity within a single microbial ecosystem Shannon Index No significant change in alpha diversity over the one-year (slightly lower in 2018) Similar results were noted with V4 Primer. 17
STABILITY OF TEMPORAL DIVERSITY Within Individual Microbiome diversity remains stable over time from 2017 and 2018 In general, subjects with high (or low) diversity in 2017 maintained their high (or low) diversity over the one-year. 2018 Shannon Diversity 4 3 2 1 0 Shannon Diversity Index 2017 vs 2018 Cheek Forehead Within Individual - Temporal Diversity is Stable 0 1 2 3 4 2017 Shannon Diversity r 2 =0.55 18
CHANGE IN MICROBIOME DIVERSITY Change in Cheek Diversity ( ) = Diversity in 2017 Diversity in 2018 Most subject s showed no change in diversity from 2017 to 2018. A few subjects showed significant increase or decrease in diversity from 2017 to 2018. Change in Shannon Diversity Index 2017-2018 2.0 1.5 1.0 0.5 0.0-0.5-1.0-1.5 Subjects rank-ordered based on change in cheek diversity ( ) Change in Skin Diversity 2017 vs 2018 Cheek Forehead Subject 19
COMMUNITY DISSIMILARITY Beta Diversity - Variability Microbiome dissimilarity between subjects is greater than the dissimilarity within a subject from 2017 to 2018. High inter-individual dissimilarity High temporal stability Distance: 0 = Identical Communities Inter-individual Community Dissimilarity > Intra- Individual 1 = Dissimilar Communities 20
RESULTS DATA ANALYSIS Facial Microbiome and Skin Parameters 21
MICROBIOME DIVERSITY vs. TEWL Subjects with better stratum corneum barrier function (lower TEWL) tend to show higher diversity, however, the relationship is very weak. Shaded areas: with 95% confidence limits. Trans-Epidermal Water Loss (TEWL) Microbiome Diversity is weakly correlated with trans-epidermal water loss 22
STABILITY OF SKIN MICROBES - FOREHEAD Forehead - Certain demographic and skin parameters were correlated with specific OTUs. Forehead, 2017 Forehead, 2018 Strongest correlation was between forehead porphyrins and Propionibacterium 23
Cheeks - 2017 Cheeks - 2018 STABILITY OF SKIN MICROBES - CHEEKS Cheeks- Stability of associations between skin parameters and microbes 24
CUTIBACTERIUM ACNES AND PORPHYRINS Good co-relation between C. acnes abundance and cheek porphyrins Abundance of C. acnes seems to be necessary for having porphyrins. Cheek Cutibacterium acnes vs Cheek Porphyrins Porphyrin Area Fraction 0.12 0.10 0.08 0.06 0.04 0.02 r 2 =0.36 0.00 0.0 0.2 0.4 0.6 0.8 1.0 Cutibacterium acnes Abundance 2017 Data: V1-V3 Subject #: 205 Let s look at this subject with high porphyrins and high C. acnes. C. acnes Abundance - Strongly Correlated with Porphyrins 25
CUTIBACTERIUM ACNES AND PORPHYRINS Subject #205 Despite very high porphyrins, visually skin showed clear complexion, healthy looking, evenness of color tone and no acne. Imaging Analysis showed very high porphyrin fluorescence redness 26
CUTIBACTERIUM ACNES AND PORPHYRINS Cheek Cutibacterium acnes vs Cheek Porphyrins Porphyrin Area Fraction 0.12 0.10 0.08 0.06 0.04 0.02 r 2 =0.36 0.00 0.0 0.2 0.4 0.6 0.8 1.0 Cutibacterium acnes Abundance 2017 Data Another person (subject #155) with High C. acnes. and High porphyrins Image Analysis Results Visually some redness Very high porphyrins fluorescence Moderate redness and red blotchiness. 27
CUTIBACTERIUM ACNES AND PORPHYRINS Although high C. acnes seems to be necessary for having porphyrins - Some subjects showed high C. acnes and low porphyrins level. Cheek Cutibacterium acnes vs Cheek Porphyrins Porphyrin Area Fraction 0.12 0.10 0.08 0.06 0.04 0.02 r 2 =0.36 0.00 0.0 0.2 0.4 0.6 0.8 1.0 Cutibacterium acnes Abundance 2017 Data: V1-V3 Subject #004 - with high C. acnes and very low porphyrins Image Analysis Clear complexion A few Porphyrins No Inflammation 28
PORPHYRINS - TEMPORAL STABILITY 2018 Cheek Porphyrin Area Fraction 0.12 0.10 0.08 0.06 0.04 0.02 Cheek Porphyrins 2017 vs 2018 r 2 =0.83 0.00 0.00 0.02 0.04 0.06 0.08 0.10 0.12 2017 Cheek Porphyrin Area Fraction Subjects with low porphyrins in 2017 showed low porphyrins in 2018 and visa versa. Let s look at this subject #003 with moderately high porphyrins in 2017 but very low porphyrins in 2018. 29
CHEEK PORPHYRIN ANALYSIS Visually - Improved Skin Health in 2018 as compared to 2017 Porphyrin Analysis Results Decrease in Porphyrins Abundance 2017-0.026 Porphyrin Area Fraction 2018-0.004 Porphyrin Area Fraction Skin Redness Analysis A marked reduction in red blotchy skin on the cheeks 30
PORPHYRINS AND C. ACNES ANALYSIS Porphyrin Area Fraction 0.030 0.025 0.020 0.015 0.010 0.005 0.000 Porphyrins declined >6-fold 2017 2018 >6X Decrease in C. acnes abundance >8-fold Cutibacterium acnes Abundance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 2017 2018 A 1:1 post-study interview with this subject revealed no obvious explanation for the dramatic decrease in porphyrins or C. acnes. >8X 31
RESULTS DATA ANALYSIS Corynebacterium kroppenstedtii (CK) Age Dependent 32
BACKGROUND: ARTPRIZE COHORT In 2015, we characterized the skin microbiomes of 500 subjects in a cross-sectional study ( ArtPrize Study ), where we found two mutually exclusive and agedependent Corynebacteria OTUs. C. Unclassified C. kroppenstedtii 33
AGE DEPENDENT CORYNEBACTERIUM 2017 OTUs 004 Cheek and the Forehead (2017 and in 2018 using V4 Primer) With age, there is a shift in abundance of Corynebacteria spp. Increase in Corynebacterium kroppenstedtii Decrease in Corynebacterium sp. (unclassified) Skin is unremarkable. Clear complexion. Similar results were noted with V1-3 Primer. 34
CORYNEBACTERIA SPP. ARE CO-EXCLUSIVE Corynebacterium kroppenstedtii and Corynebacterium unclassified are usually Co-exclusive in their abundance C. unclassified Abundance 1.0 0.8 0.6 0.4 0.2 Corynebacterium kroppenstedtii vs. Corynebacterim unclassified Abundance 0.0 0.0 0.2 0.4 0.6 0.8 1.0 C.kroppenstedtii Abundance 35
C. KROPPENSTEDTII - TEMPORAL STABILITY Corynebacterium kroppenstedtii - High intra-individual and temporal stability.. If relative abundance of C. kroppenstedtii was low in 2017, it remained low in 2018 and visa versa. 2018 C. kroppenstedtii Abundance 1.0 0.8 0.6 0.4 0.2 Corynebacterium kroppenstedtii Abundance 2017 vs 2018 r 2 =0.52 0.0 0.0 0.2 0.4 0.6 0.8 1.0 2017 C.kroppenstedtii Abundance 36
C. KROPPENSTEDTII - TEMPORAL STABILITY Based on C. kroppenstedii abundance, the subjects were grouped into: < 0.1 relative abundance Low C. Kropp. > 0.1 relative abundance High C. Kropp. The skin parameters (age, BMI, Redness etc.) of these two groups were compared using a t-test. 2018 C. kroppenstedtii Abundance 1.0 0.8 0.6 0.4 0.2 Corynebacterium kroppenstedtii Abundance 2017 vs 2018 r 2 =0.52 0.0 0.0 0.2 0.4 0.6 0.8 1.0 2017 C.kroppenstedtii Abundance 37
Skin Measurements C. KROPPENSTEDII - ABUNDANCE Parameter Low C. kropp. (<0.1) High C. kropp. (>0.1) P-value The group with higher Corynebacterium kroppenstidii (>0.1) had significantly higher Skin Redness (2.5a*) Age 42 50 0.001 BMI 26.2 28.9 0.03 Red Features 0.08 0.11 2E-05 Visible Spots 0.036 0.045 0.02 Red Spots 0.005 0.008 0.004 Redness 0.39 0.51 0.005 Evenness 0.51 0.47 0.001 a* 23.1 25.6 0.0002 L* 64.7 63.1 0.001 Increase in Corynebacterium kroppenstedtii- Increase in Skin Redness 38
C. KROPPENSTEDII - LIETERATURE REVIEW In Rosacea Patients as compared to controls, the cheek and nose data showed that the relative abundance was significantly; Higher for Corynebacterium kroppenstedtii (4.35; p< 0.005) Nose Higher for Finegoldia magna (+4.44 log; p<0.005) Cheek Lower for Propionibacterium acnes (-3.48 log; p<0.005) - Cheek Rosacea Patients: C. kroppenstedtii and F. magna may have important roles in rosacea pathophysiology 39
SKIN MICROBIOTA DYSBIOSES - Shift in skin microbiota of Western European women across aging R. Jugé et al, 23 May 2018. Journal of Applied Microbiology - R&D department, SILAB, Brive la Gaillarde, France Concluded: At the genus level, older skin exhibited: A significant increase in Corynebacterium and a decrease in Propionibacterium relative abundance. Cinco De Mayo Study - - With increase in age- Increase in C. kroppenstedii. - With increase in C. kroppenstedii abundance of - Increase in Skin Redness Rosacea Patients (Literature) - Higher abundance of C. kropppenstedii and Finegoldia magna - Lower P. acnes Food for thoughts: Is Rosacea a result of dysbiosis i.e. high abundance of C. kroppenstedii and F. magna and low P. acnes? 40
SUMMARY Results observed in two separate cohorts, multiple skin sites, two different primers (V4 and V1-V3) and over 1 year period. 1. Microbiome diversity and composition is relatively STABLE (over 1 year in this study cohort). 2. Microbiome diversity and composition are significantly associated with important parameters of skin health (e.g., redness, porphyrins). 3. Corynebacterium kroppenstedtii is: Associated with age and skin redness parameters. Mutually exclusive with other Corynebacterium spp. (unclassified). 41
THANK YOU! Skin Microbiome Team: Greg Hillebrand PhD, Brandon Iker, PhD Amway Clinical Team Pedro Dimitri, PhD Bill Mohn, PhD 42