Locard s Exchange Principle

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Forensic Science http://media.popularmechanics.com/images/pmx0706forensicshairsmall.jpg Presentation developed by T. Tomm 2006 http://sciencespot.net/ Locard s Exchange Principle "Every Contact Leaves a Trace" The value of trace (or contact) forensic evidence was first recognized by Edmund Locard in 1910. He was the director of the very first crime laboratory in existence, located in Lyon, France. The Locard s Exchange Principle states that "with contact between two items, there will be an exchange." For example, burglars will leave traces of their presence behind and will also take traces with them. They may leave hairs from their body or fibers from their clothing behind and they may take carpet fibers away with them. Source: http://www.virtualsciencefair.org/2004/fren4j0/public_html/trace_evidence.htm 1

Biology of Hair Hair is composed of the protein keratin, which is also the primary component of finger and toe nails. Hair is produced from a structure called the hair follicle. Humans develop hair follicles during fetal development, and no new follicles are produced after birth. Hair color is mostly the result of pigments, which are chemical compounds that reflect certain wavelengths of visible light. Hair shape (round or oval) and texture (curly or straight) is influenced heavily by genes. The physical appearance of hair can be affected by nutritional status and intentional alteration (heat curling, perms, straightening, etc.). In order to test hair evidence for nuclear DNA, the root must be present. Hair may also be tested for mitochondrial DNA, or DNA that is found in the mitochondria of our cells. NOTE: Hair that normally falls out does not have a root attached, while hair that is pulled out by force does. Sources: http://library.thinkquest.org/04oct/00206/lesson.htm#t_hair & http://www.fbi.gov/hq/lab/fsc/backissu/july2000/deedric1.htm#index%20(hairs) Image: https://dps.mn.gov/divisions/bca/bca-divisions/forensic-science/pages/trace-hair.aspx Hair Structure = Pencil Cuticle outer coating composed of overlapping scales Cortex protein-rich structure around the medulla that contains pigment Medulla central core (may be absent) The structure of hair has been compared to that of a standard pencil with the medulla being the lead, the cortex being the wood and the cuticle being the paint on the outside. http://library.thinkquest.org/04oct/00206/lesson.htm#t_hair 2

Hair Structure Cuticle Outer coating composed of overlapping scales The cuticle varies in: Its scales, How many there are per centimeter, How much they overlap, Their overall shape, and How much they protrude from the surface Its thickness, and Whether or not it contains pigment. Characteristics of the cuticle may be important in distinguishing between hairs of different species but are often not useful in distinguishing between different people. Info: http://library.thinkquest.org/04oct/00206/lesson.htm#t_hair Image: http://www.hairdressersus.com/micro/image5b.jpg Hair Structure Cortex Protein-rich structure around the medulla that contains pigment The cortex varies in: Thickness Texture Color Distribution of the cortex is perhaps the most important component in determining from which individual a human hair may have come. Microscopic examination can also reveal the condition and shape of the root and tip. Info: http://library.thinkquest.org/04oct/00206/lesson.htm#t_hair Image: http://www.extrapersonality.com/hair.html 3

Hair Structure Medulla Central core (may not be present in all hair samples) The medulla may vary in: Thickness Continuity - one continuous structure or broken into pieces Opacity - how much light is able to pass through it It may also be absent in some species. Like the cuticle, the medulla can be important for distinguishing between hairs of different species, but often does not lend much important information to the differentiation between hairs from different people. http://library.thinkquest.org/04oct/00206/lesson.htm#t_hair http://www.bfro.net/images/whatis/figures/fig.%203%20with%20caption.jpg Let s take a closer look 1- Get a plastic sheet from your teacher. 2 Pull out a strand of hair and place it inside the plastic sheet. 3 - Have it laminated by the teacher and write your name on it. 4 - Use a microscope to examine your hair sample. Change the powers of magnification (low medium high) and use the focus to observe the different layers of hair and the root. 5 Compare your hair sample to those of your classmates. Done? Example the animal hair samples! How do they compare to your hair sample? 4

Fiber Evidence A fiber is the smallest unit of a textile material that has a length many times greater than its diameter. A fiber can be spun with other fibers to form a yarn that can be woven or knitted to form a fabric. The type and length of fiber used, the type of spinning method, and the type of fabric construction all affect the transfer of fibers and the significance of fiber associations. This becomes very important when there is a possibility of fiber transfer between a suspect and a victim during the commission of a crime. Matching unique fibers on the clothing of a victim to fibers on a suspect s clothing can be very helpful to an investigation, whereas the matching of common fibers such as white cotton or blue denim fibers would be less helpful. The discovery of cross transfers and multiple fiber transfers between the suspect's clothing and the victim's clothing dramatically increases the likelihood that these two individuals had physical contact. http://www.fbi.gov/hq/lab/fsc/backissu/july2000/deedric3.htm#fiber%20evidence Natural Fibers Many different natural fibers that come from plants and animals are used in the production of fabric. Cotton fibers are the plant fibers most commonly used in textile materials The animal fiber most frequently used in the production of textile materials is wool, and the most common wool fibers originate from sheep. http://www.fireflydiapers.com/articles/diaperarticle_naturalfibersabsorb.htm 5

Synthetic Fibers More than half of all fibers used in the production of textile materials are synthetic or man-made. Nylon, rayon, and polyester are all examples of synthetic fibers. Cross-section of a man-made fiber Fibers under a microscope Images: http://www.trashforteaching.org/phpstore/product_images/yarnws.jpg http://www.fbi.gov/hq/lab/fsc/backissu/july2000/deedric3.htm#fiber%20evidence http://www.jivepuppi.com/images/fiber_evidence.jpg 6