AcquaSeal Coconut Anti-Aging, Nourishing, Moisturization, Improves Slip. Tomorrow s Vision Today!

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AcquaSeal Coconut Anti-Aging, Nourishing, Moisturization, Improves Slip Tomorrow s Vision Today!

AcquaSeal Coconut Technical Information Product Code: 20742 INCI Name: Cocos Nucifera (Coconut) Fruit Extract INCI Status: Conforms Suggested Use Level: 1.0-5.0% Suggested Applications: Anti-Aging, Nourishing Moisturization, Improves Slip

Coconut Water Trend The use of Coconut Water in Health & Wellness Market: o Coconut water is the most rapidly growing wellness-oriented beverage o Exotic and has excellent consumer recognition Coconut water has an enduring history of use in medical applications: o Isotonic Solution Replaces lost fluids & minerals when the body is dehydrated

Coconut Water in Cosmetics Revlon Lush Paul Pender o Brands are capitalizing on the coconut water trends o Example: Hydrating Make-Up Active Concepts asks: In topical applications, does coconut water really hydrate the skin? Coconut water is not how the coconut stays hydrated!

Coconut Lipids Coconuts answer to Moisture Retention: Coconut water is found suspended in the hydrophobic meat, or white/fleshy edible part of the coconut Rich in MCT s Contributing to cellular nutrition Effective Levels of Sterols Structurally similar to cholesterol an essential component of proper cell membrane permeability & fluidity MCT & Sterol constituents Provide protection to the internal coconut water & dehydrating elements

Percent (%) Increase 180 160 140 120 100 80 60 40 20 Increase in Moisturization 0 t = 0 24 hrs 1 week 2 weeks 3 weeks Experimental (2.0% AcquaSeal Coconut in Base Lotion) Untreated Control Base Lotion Figure 1: Improvements in moisturization following application of the test materials after a period of 3 weeks. Protocol Equipment: DermaLab Combo Principle of measurement: Conductance, single frequency Subjects: 10 (m/f) Test area: Volar forearms Concentration of active used: 2.0% Frequency of application: Twice Daily

Percent (%) Difference 180% 160% 140% 120% 100% 80% 60% 40% 20% 0% -20% Moisturization Comparison 168% 29% 138% 31% 122% Figure 2: Improvements in moisturization following application of the test materials after a period of 3 weeks. 108% 35% 33% -3% -2% t = 0 24 hrs 1 week 2 weeks 3 weeks Experimental vs. Untreated Control Experimental vs. Base Lotion Protocol Equipment: DermaLab Combo Principle of measurement: Conductance, single frequency Subjects: 10 (m/f) Test area: Volar forearms Concentration of active used: 2.0% Frequency of application: Twice Daily

Percent (%) Increase 160 140 120 100 80 60 40 24 Hour Moisturization Study Protocol Conducted over a period of 24 hours Ten (M/F) subjects Equipment: DermaLab Corneometer Moisture levels were improved by 150% after 24 hours when compared to the untreated control When compared to the base lotion, AcquaSeal Coconut improved moisturization by 14% after 24 hour hours 20 0-20 T = 0 Base Lotion - Untreated Control AcquaSeal Coconut - Base Lotion 24 hrs AcquaSeal Coconut - Untreated Control Figure 7. Percent increase in moisturization 24 hours after application of AcquaSeal Coconut and base lotion

0 Decrease in TEWL t = 0 1 week 2 week 3 weeks Protocol Equipment: DermaLab Combo Percent (%) Decrease -2-4 -6-8 -10-12 -14-16 -18 Principle of measurement: Open Chamber, vapor diffusion gradient Subjects: 10 (m/f) Test area: Volar forearms Concentration of active used: 2.0% -20 Experimental (2.0% AcquaSeal Coconut in Base Lotion) Untreated Control Base Lotion Figure 3: Improvements in barrier function following application of the test materials after a period of 3 weeks. Frequency of application: Twice Daily

TEWL Comparison t = 24 1 week 2 week 3 weeks 20% 19% 18% Protocol Equipment: DermaLab Combo Percent (%) Difference 16% 14% 12% 10% 8% 6% 4% 10% 6% 11% 9% 12% 11% 15% Principle of measurement: Ultrasound Probe Subjects: 10 (m/f) Test area: Volar forearms 2% 0% Concentration of active used: 2.0% Experimental vs. Untreated Control Experimental vs. Base Lotion Figure 4: Improvements in barrier function following application of the test materials after a period of 3 weeks. Frequency of application: Twice Daily

Percent (%) Increase 350 300 250 200 150 100 50 Hydration Potential Protocol Measured according to the British Pharmacopoeia (BP) water absorption capacity method Sample materials were placed separately into a mortar and water was added AcquaSeal Coconut is an effective allnatural and botanical alternative to Lanolin as it is capable of holding 200% of its weight in water AcquaSeal Coconut exhibit superior hydration potential with respective improvements in water holding capacity of 28%, 967%, and 3100% 0 AcquaSeal Coconut Lanolin Avocado Sterols Petrolatum Jojoba Oil Figure 6. Hydration Potential Results.

Half Head Study Protocol 5 participants with ethnic hair Test area: Hair Concentration of active used: 2.0% Principle of measurement: Salon professional and volunteer assessed hair characteristics AcquaSeal Coconut demonstrated the ability to smooth the cuticle of the hair, enhance combability, and increase shine AcquaSeal Coconut is also ideal for ethnic hair

Half Head Study Base Shampoo and Conditioner 2.0% AcquaSeal Coconut + Base Shampoo/ Base Conditioner Figure 1. Full Head Baseline Photo of Untreated Hair. Figure 2. Half-Head Photo of hair treated with test and control shampoo/conditioner

Half Head Study Base Shampoo and Conditioner 2.0% AcquaSeal Coconut + Base Shampoo/ Base Conditioner Figure 1. Full Head Baseline Photo of Untreated Hair. Figure 2. Half-Head Photo of hair treated with test and control shampoo/conditioner

Half Head Study Base Shampoo and Conditioner 2.0% AcquaSeal Coconut + Base Shampoo/ Base Conditioner Figure 1. Full Head Baseline Photo of Untreated Hair. Figure 2. Half-Head Photo of hair treated with test and control shampoo/conditioner

Half Head Study Base Shampoo and Conditioner 2.0% AcquaSeal Coconut + Base Shampoo/ Base Conditioner Figure 1. Full Head Baseline Photo of Untreated Hair. Figure 2. Half-Head Photo of hair treated with test and control shampoo/conditioner.

Half Head Study Base Shampoo and Conditioner 2.0% AcquaSeal Coconut + Base Shampoo/ Base Conditioner Figure 1. Full Head Baseline Photo of Untreated Hair. Figure 2. Half-Head Photo of hair treated with test and control shampoo/conditioner.

ORAC Assay Protocol 18 16 17 Solutions of AcquaSeal Coconut and Trolox (positive control) were prepared in a 1:1 Acetone:DiH 2 O 14 Concentrations: 0.5%, 0.1%, 0.05% Antioxidant Capacity (µmte) 12 10 8 6 4 2 0 2 12.5µM Trolox 6.25µM Trolox 0.5% 20742 AcquaSeal Coconut 9 4 0.1% 20742 AcquaSeal Coconut 2 0.05% 20742 AcquaSeal Coconut 25µL of test material and Trolox were combined with 150µL of fluorescein in 75mM potassium phosphate buffer and incubated in the Synergy HT Microplate reader at 37 C for 30 minutes Measurements were taken every 2 minutes for 2 hours AcquaSeal Coconut began exhibiting antioxidant activity at a 0.05% concentration Figure 5: Antioxidant capacities.

AcquaSeal Coconut Safety Data Additional Data: Dermal and Ocular Irritation Test: Non-Irritating

AcquaSeal Coconut: Benefits Aesthetic Enhancement Enhances the feel of formulations Natural replacement for Petrolatum Topically-active; Oil Soluble Hydrophobic materials penetrate & easily assimilate to the skins environment Water Soluble products do not High consumer recognition Exotic & trendy Renewable & Innovative Great for hair care

ACTIVE CONCEPTS LLC THANK YOU For more information Visit our website! www.activeconceptsllc.com