ANTI-WRINKLES Epidermic homeostasis for an anti-wrinkle effect
Epidermic homeostasis for an anti-wrinkle effect ANTI-WRINKLES Wrinkles: alterations in the ECM homeostasis Keratinocytes release cytokines (IL-1α and TGF-β) that regulate fibroblastic activity. A balance is established between extracellular matrix (ECM) basal synthesis (TGF-β) and basal degradation (IL-1α), thus generating a state of HOMEOSTASIS. Line Wrinkle Fold Epidermis Dermis Subcutis Processes that alter the balance of the ECM homeostasis, consequently produce wrinkles and result in a less elastic, less firm and less moisturized skin. Restoring homeostasis reduces wrinkles and recovers the cosmetic properties of skin. TGF-β ECM DEGRADATION HOMEOSTASIS ECM SYNTHESIS IL-1α recovers and maintains homeostasis in the dermal extracellular matrix (ECM), which results in a reduced amount of wrinkles Homeostatine TM features and benefits is composed by a 3D Matrix of Andean galactomannans, which sequentially releases a marine pentasaccharide from Enteromorpha compressa. Its applications on the skin trigger a cascade of effects that eventually restore the extracellular matrix homeostasis. By restoring ECM balance: Increases synthesis of collagen I and III. Increases skin thickness. Increases the elastic fibers. Increases glucosaminoglycans. Reduces metalloproteinases. Inhibits the IL-1α stimulus. PL56 HM56 Illustration of the dermo-epidermal junction in (HM56) treated skin and placebo (PL56) treated skin. actions: Wrinkle reduction Improvement of skin firmness, moisturization and elasticity Anti-aging
Epidermic homeostasis for an anti-wrinkle effect In vitro efficacy Study conducted on primary cultures of human dermal fibroblasts (HDF) to evaluate the effects by on extracellular matrix proteins synthesis (collagen I and III) and IL-1α action. Collagen I synthesis.1%.2%.4% TGF-ß 2 ng/ml Collagen III synthesis.1%.2%.4% TGF-ß 2 ng/ml % INCREASE VS. CONTROL 141% 164% 184% 5 1 15 2 25 % INCREASE VS. CONTROL 25% 74% 114% 5 1 15 IL-8 inhibition in IL-1α in activated fibroblasts.2% % INHIBITION VS. CONTROL 243% 144% 42.9% In vivo efficacy 12 female volunteers applied 2% cream twice a day in one periorbital area and a placebo-cream on the other one, for 56 days. Evaluation of wrinkles using the FOITS technique. Wrinkle reduction -2. -4. -6. -8. -1. % REDUCTION OF WRINKLES PLACEBO 2% -2.33% -2.28% SPt = Main wrinkle -9.59% -7.14% VITAMIN C 3% -5.4% SPa = Average rugosity wrinkle reduction 3% higher than that produced by vitamin C Rejuvenating effect 6. 4. 2. -2. -4. % INCREASE OF SKIN THICKNESS PLACEBO -3.11% 5.4% 2% PDTC 5uM 37.7% 34 36 38 4 42 44 increases 8.15% the skin thickness
Red light & Homeostatine Beauty devices have become part of daily beauty routines. To fight aging at home, one of the best and most used techniques is red light. Red light devices Many studies have demostrated the ability of red light to reach the dermis and activate diferent mechanisms to counteract skin aging: Increases synthesis of pro-collagen and collagen Inhibits the overexpression of MMP1 Increases the fibroblast migration Visible anti-wrinkles effects Improvement in microcirculation Better together: Homeostatine + Red light The anti-aging activity of homeostatine made us think that it could work in synergy with red light, and results showed how the efficacy was better together than any of them separately. Technology and nature are the best combination, the winning alliance for: - antiaging effect - rejuvenating and photorejuvenating - anti-wrinkles - firming - regenerating - alternative to surgery without side effects Safe, non-invasive, painless, at home solution to combat skin aging and photoaging HOMEOSTATINE SKIN
ANTI-WRINKLES In vitro efficacy: Homeostatine TM + Red light To demonstrate the synergy between our active homeostatine and red light, human dermal fibroblasts were stimulated with 66nm red light together with homeostatine. These cells are responsible for the synthesis of collagen, essential in the dermal structure to maintain a youthful skin and the balance of MMPs to avoid their overexpression that leads to aging. Increase of collagen I and III gene expression We analysed gene expression of collagen types I and III after preincubation with (.2%) and stimulation with red light: Increase in collagen synthesis We studied the quantity of total collagen and collagen type III after stimulation and (.2%) incubation: NO NO 4 3 2 1 89 189 37 145 15 1 5 11 22 2 18 25 Collagen type I Collagen type III Total Collagen Collagen type III The result of this increment of collagen is a rejuvenated looking skin. Decrease in MMP1 enzyme synthesis MMP 1 enzyme (collagenase) degrades collagen fibers. We study the secretion of this enzyme after stimulation and (.2%) incubation: Fibroblast migration We studied the effect of the red light and (.1%) on fibroblasts migration within an exclusion zone: NO NO 11 9 7-1 -5-27 2 15 2 55 64 5 1 3 1 5 The overexpression of MMPs related to aging is reduced. Increased migration means a regenerated skin. Homeostatine improves the performance of red light devices
Epidermic homeostasis for an anti-wrinkle effect ANTI-WRINKLES cosmetic applications Technical specifications Boosts the anti-aging effect of red light Anti-wrinkle treatment for aged skin First wrinkles treatment ECM rejuvenating (firmness, elasticity and moisturizing) 7228 PROPERTIES ACTIVE MOLECULES APPEARANCE SOLUBILITY Red light booster Anti-wrinkles, firming, moisturizing Balances skin homeostasis Stimulates dermal metabolism and improves skin elasticity Marine pentasaccharide (MPS) Liquid Pale yellow color Soluble in aqueous solutions B C D F Dimethicone/Divinyl Dimethicone/Silsesquioxane Crosspolymer, Copernica Cerifera (Carnauba) Wax Cyclopentasiloxane, Polysilicone-11 Polysorbate 8 Tocopheryl Acetate Aqua (Water), Sodium Hydroxide Alcohol Denat. Parfum (Fragrance) Lemon Oil trikenol TM 2.5 1..5.5.8 15..15 E Polyacrylate-13, Polyisobutene, Polysorbate 2 2.75 1..1 5. RECOMMENDED DOSE 2-5 % G Aqua (Water), Alcohol Denat, CI 429.1 INCI Formulation Anti-wrinkle Men Creamy Gel A INCI / PCPC Aqua, glycerin, enteromorpha compressa extract, caesalpinia spinosa gum, sodium benzoate, potassium sorbate (other versions available in solvents and preservatives) Aqua (Water) Acrylates/C1-3 Alkyl Acrylate Crosspolymer Polyacrylate-14 Glycerin Caprylyl Glycol Panthenol % (w/w) 57.14.25 1.5 2..3.5 Rejuvenating Booster Gel A B C D E INCI / PCPC Aqua (Water) Tetrasodium Glutamate Diacetate, Sodium Hydroxide, Aqua (Water) Potassium Sorbate Sodium Benzoate Pronalen Fruit Acids AHA-5 Ammonium Acryloyldimethyltaurate/VP Copolymer 1,2-Hexanediol Glycerin zirhafirm TM M.S. Aqua (Water), Sodium Hydroxide % (w/w) 86.6.2.2.2.5 1.5.5 7. 1. 2..3 V2-216