Supporting Information: Paraben concentrations in maternal urine and breast milk and its association with personal care product use. Mandy Fisher 1*, Susan MacPherson 1, Joseph M. Braun 2, Russ Hauser 3, Mark Walker 4, Mark Feeley 5, Ranjeeta Mallick 4, René Bérubé 6, Tye E. Arbuckle 1 1Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, ON 2 Department of Epidemiology, Brown University, Providence, RI 02912 3Harvard University School of Public Health, Boston, MA, USA. 4The Ottawa Hospital Research Institute, Ottawa, ON, Canada 5Health Products and Food Branch, Health Canada, Ottawa, ON 6Institut National de Santé Publique du Québec, QC, Canada *Corresponding Author: Mandy Fisher Address: 50 Colombine Driveway, Tunney s Pasture. Ottawa Canada. K1A 0K9 Email: mandy.fisher@canada.ca Phone: +1 613 301 5618 Number of pages: 12 Number of Figures: 2 Number of Tables: 6 S1
Table S1: Validation data of the ID-LC-MS/MS methods for the determination of parabens in urine and milk samples. Analyte Matrix LOD LOQ Calibration Recovery Matrix Intraday range effect Precision Concentration a (µg/l) (µg/l) (µg/l) (%) (%) (%) (µg/l) BP U 0.025 0.084 LOQ to 200 96 90 3.3 0.25 M 0.024 0.078 LOQ to 50 80 110 3.8 0.2 MP U 0.1 0.35 LOQ to 200 94 91 1.7 2.1 M 0.04 0.013 LOQ to 50 69 110 7.2 0.18 PP U 0.081 0.27 LOQ to 200 98 93 4.1 0.65 M 0.026 0.087 LOQ to 50 79 99 7 0.12 EP U 0.11 0.38 LOQ to 200 92 99 2.7 1.4 M 0.03 0.1 LOQ to 50 75 110 8.3 0.12 IBP U 0.034 0.11 LOQ to 200 95 97 3.9 0.3 M 0.024 0.81 LOQ to 50 80 100 4.3 0.19 BzP U 0.054 0.18 LOQ to 200 85 103 5.5 0.33 M 0.022 0.074 LOQ to 50 79 93 3.9 0.19 Abbreviations: BP, butylparaben; MP, methylparaben; PP, n-propylparaben; EP, ethylparaben; IBP, isobutylparaben; BzP, benzylparaben; U, urine; M, milk a Concentration of the analyte in the quality control material S2
Table S2: Urine Sample Characteristics (n = 542 urine samples collected from 31 women) N % season of sampling spring 138 25.46 summer 101 18.63 autumn 170 31.37 winter 133 24.54 Time since last void 0-86 minutes 127 25.15 87-134 minutes 127 25.15 135-199 minutes 127 25.15 >200 minutes 124 24.55 missing 37 Time of Day midnight to 7:59 118 22.10 8:00 to 11:59 100 18.73 12:00 to 17:59 166 31.09 18:00-23:59 150 28.09 missing 8 Total Volume <40mL 26 4.81 40 to <65 ml 155 28.65 65 to <90 ml 112 20.70 >=90mL 248 45.84 missing 1 Study Visit T1A (weekday) 270 49.82 T1B (weekend day) 272 50.18 S3
Table S3: Prevalence of Product Use (total # and % of women) in 24-h sampling period (N=31 women) Product Group Abbreviation Examples of products in this category T1a Day1 (n=31) T1a Day2 (n=30) Deodorant and Antiperspirants Baby Lotions, Soaps and other Baby Products Body Soaps Conditioner Eye Makeup n % n % anti Deodorant, antiperspirant 18 58.06 17 56.67 babyprod bodysoap condit Face Lotions and Creams face Fragrance and Perfumed Products Hairstyling Products eyemakeup frag hair Baby wipes, diaper ointment, baby body wash, petroleum jelly Body wash, body gel, shower soap Rinse-off conditioner, 2-in-1 shampoo and conditioner, leave-in conditioner Eye shadow, mascara, brow liner Day cream, night lotion, acne cream, eye moisturizer perfume, bubble bath, body mist, air freshener Hairspray, gel, mousse, hair paste 10 32.26 5 16.67 21 67.74 25 83.33 17 54.84 17 56.67 18 58.06 17 56.67 21 67.74 19 63.33 3 9.68 6 20.00 7 22.58 13 43.33 Lip Products lip lip balm, lipstick, lip gloss 10 32.26 12 40.00 Body Lotions, Creams and Oils General Makeup and Cosmetics lotion Nail Polish and Remover nail Toothpaste and Mouthwash Body butter, belly oil, sunscreen, hand cream 22 70.97 17 56.67 makeup Blush, foundation, concealer 15 48.39 17 56.67 oral nail polish, nail polisher remover Toothpastes, dental rinse, mouthwash 0 0 0 0 31 100.00 27 90.00 Shampoo shampoo shampoo 17 54.84 21 70.00 Facial Soaps, Cleansers and Washes Hand Soaps, Sanitizers and Soap Not Otherwise Specified (NOS) skinclea soap Face cleanser, exfoliator, face masks, eye makeup remover Liquid soap, bar soap, waterless sanitizers, generic soap 19 61.29 18 60.00 15 48.39 13 43.33 S4
Table S4: Adjusted geometric means (GM) in maternal urine (ug/dl) by Product Use 24h before urine sampling anti frag oral condit shampoo bodysoap soap eyemakeup Product Group No (n=262) Yes (n=267) No (n=476) Yes (n=53 ) No (n=40) Yes (n= 489) No (n=261) Yes (n= 268) No (n=228) Yes (n=301) No (n=171) Yes (n= 358) No (n=304) Yes (n=225 ) No (n=229) Yes (n=230 ) makeup No (n=300) GM (95% CI) 0.16 (0.06,0.42) 0.27 (0.1,0.72) 0.17 (0.07,0.41) 0.96 (0.31,3.04) 0.16 (0.05,0.52) 0.21 (0.08,0.53) 0.15 (0.06,0.39) 0.27 (0.11,0.69) 0.15 (0.06,0.38) 0.27 (0.11,0.69) 0.16 (0.06,0.41) 0.24 (0.09,0.61) 0.27 (0.1,0.73) 0.14 (0.05,0.39) 0.17 (0.07,0.42) 0.29 (0.11,0.76) 0.17 (0.07,0.42) BP EP MP PP % Change p- value 69.52 0.0430 460.91 0.0001 27.91 0.5287 76.30 0.0069 84.30 0.0021 52.73 0.0700-47.97 0.0124 73.42 0.0650 GM (95% CI) 2.22 (1.25,3.95) 2.54 (1.43,4.51) 2.25 (1.27,3.99) 3.77 (1.97,7.23) 1.49 (0.79,2.82) 2.48 (1.42,4.33) 2.28 (1.29,4.04) 2.48 (1.4,4.4) 2.24 (1.26,3.98) 2.49 (1.41,4.4) 2.06 (1.15,3.69) 2.55 (1.45,4.5) 2.36 (1.34,4.18) 2.4 (1.35,4.27) 2.26 (1.27,4) 2.55 (1.43,4.54) 2.41 (1.35,4.3) % Chan ge p- value 14.22 0.2956 67.49 0.0052 66.38 0.0048 8.85 0.4728 11.06 0.3257 23.96 0.0801 1.58 0.8956 12.74 0.3513 GM (95% CI) 28.77 (18.49,44.77) 32.12 (20.66,49.93) 29.55 (19.19,45.5) 38.9 (22.83,66.29) 25.65 (15.09,43.59) 30.85 (20.17,47.17) 30.28 (19.47,47.1) 30.51 (19.62,47.44) 29.48 (18.96,45.85) 31.11 (20.12,48.1) 28.63 (18.18,45.08) 31.3 (20.3,48.26) 23.49(15.38, 35.9) 42.91 (27.86,66.09) 31.6 (20.3,49.18) 28.96 (18.45,45.44) 35.28 (22.33,55.73) % Chan ge p- value 11.62 0.3873 31.67 0.1380 20.27 0.3093 0.75 0.9495 5.53 0.6156 9.33 0.4697 82.62 <0.000 1-8.36 0.4974 GM (95% CI) 4.91 (2.91,8.27) 4.36 (2.59,7.35) 4.53 (2.71,7.56) 5.54 (2.89,10.63 ) 3.53 (1.84,6.75) 4.73 (2.86,7.83) 4.75 (2.8,8.04) 4.5 (2.66,7.61) 4.43 (2.62,7.51) 4.77 (2.84,8.02) 3.89 (2.26,6.71) 5.02 (3,8.41) 3.4 (2.06,5.62) 6.96 (4.17,11.63 ) 4.75 (2.79,8.09) 4.46 (2.59,7.68) 4.54 (2.66,7.77) % Change p-value -11.03 0.4716 22.39 0.3950 34.01 0.2082-5.30 0.7193 7.54 0.5972 29.00 0.1073 104.75 <0.000 1-6.15 0.7005 S5
Yes (n= 229) 0.29 2.34 25-4.73 72.35 0.0944-2.98 0.8478 0.0272 (0.11,0.76) (1.3,4.22) (15.62,39.99) 29.15 (2.72,8.24) 4.12 0.8405 babyprod No (n=421) 0.22 2.2 30.06 4.46 (0.08,0.56) (1.25,3.87) (19.59,46.13) (2.67,7.46) Yes (n=108) 0.18 3.18 31.97 5.3-19.14 0.4911 44.94 0.0099 6.33 0.6706 (0.06,0.5) (1.75,5.81) (19.88,51.4) (2.98,9.45) 18.92 0.3487 face No (n=231) 0.2 2.75 35.76 5.12 (0.08,0.54) (1.53,4.94) (22.95,55.73) (3,8.74) Yes (n= 298) 0.21 2.13-26.96-4.29 3.76 0.8992 0.0663 0.0417 (0.08,0.55) (1.2,3.8) 22.54 (17.49,41.55) 24.61 (2.55,7.2) -16.32 0.3173 lotion No (n=197) 0.13 1.57 19.76 3.21 (0.05,0.35) (0.89,2.78) (12.65,30.85) (1.87,5.51) Yes (n=332) 0.28 3.06 <0.000 39.5 <0.000 5.76 109.62 0.0055 94.68 99.92 (0.11,0.72) (1.75,5.35) 1 (25.69,60.73) 1 (3.43,9.68) 79.53 0.0003 nail 0.21 2.39 30 4.56 No (n=526) (0.08,0.52) (1.36,4.18) (19.53,46.08) (2.74,7.59) 1.04-419.1(141.2, 1297. <0.000 64.41(15.9 Yes (n=3) NA NA NA 0.1125 1312.56 0.0001 (0.33,3.31) 56.27 1243.9) 04 1 8,259.56) hair No (n=342) 0.24 2.3 28.49 4.46 (0.09,0.63) (1.3,4.06) (18.42,44.09) (2.66,7.48) Yes (n=187) 0.14 2.53 34.32 4.93-41.96 0.0330 9.89 0.4422 20.44 0.1292 (0.05,0.38) (1.41,4.53) (21.77,54.1) (2.87,8.48) 10.55 0.5250 skinclea No (n=223) 0.2 3.16 31.6 5.32 (0.08,0.53) (1.77,5.65) (20.16,49.52) (3.09,9.17) Yes (n= 306) 0.21 1.93-29.58 4.17 6.45 0.8140 0.0001-6.40 0.6082 (0.08,0.55) (1.09,3.43) 38.85 (19.07,45.86) (2.46,7.08) -21.67 0.1392 lip No (n=371) 0.24 2.58 30.1 4.38 (0.09,0.62) (1.46,4.57) (19.49,46.49) (2.61,7.35) Yes (n=158) 0.15 1.96-31.17 5.24-36.75 0.1249 0.0448 3.55 0.8002 (0.05,0.42) (1.08,3.55) 24.05 (19.58,49.61) (3.0,9.16) 19.55 0.3119 *n=number of samples (i.e.# of samples where oral products were used 24h before) *Separate models were used for each predictor and outcome and specific gravity was included as a covariate in all models. Models were also adjusted study visit, time of urine collection, BMI, job category, season and number of other product categories used were also retained in the model when statistically significant. S6
Table S5: Adjusted geometric mean urinary concentrations according to timing of most recent personal care product use among pregnant women in the P4 Study. BP EP MP PP Product Group N GM (95% CI) p-value GM (95% CI) p-value GM (95% CI) p-value GM (95% CI) p-value anti frag oral condit shampoo bodysoap 0-6 Hours 76 0.35(0.13,0.94) 0.0110 2.56(1.39,4.72) 0.4191 39.28(24.59,62.76) 0.3690 6.4(3.65,11.21) 0.4433 6-24 Hours 191 0.23(0.09,0.59) 0.1038 2.05(1.13,3.73) 0.0232 26.68(17.07,41.71) 0.1636 3.5(2.05,5.95) 0.0372 > 24 Hours 77 0.21(0.08,0.55) 0.1455 1.78(0.96,3.29) 0.0013 23.27(14.57,37.16) 0.0203 4.31(2.46,7.55) 0.2657 No Use 190 0.13(0.05,0.33) 2.95(1.61,5.41) 33.45(21.12,52.98) 5.38(3.11,9.31) 0-6 Hours 10 1.99(0.46,8.63) 0.0001 4.16(1.89,9.15) 0.0357 60.07(29.91,120.65) 0.0130 8.19(3.44,19.51) 0.1094 6-24 Hours 43 0.81(0.25,2.56) 0.0006 3.87(2,7.5) 0.0057 36.21(20.88,62.8) 0.2524 5.06(2.59,9.88) 0.6279 > 24 Hours 9 0.24(0.05,1.22) 0.5974 2.47(1.01,6.04) 0.7797 35.49(15.57,80.91) 0.5787 4.57(1.63,12.85) 0.9649 No Use 472 0.16(0.07,0.36) 2.23(1.27,3.94) 28.93(18.72,44.71) 4.48(2.69,7.46) 0-6 Hours 182 0.24(0.1,0.56) 0.0660 2.52(1.43,4.46) 0.0000 33.13(21.5,51.04) 0.0229 5.77(3.43,9.72) 0.0502 6-24 Hours 307 0.19(0.08,0.44) 0.1354 2.55(1.45,4.48) 0.0000 29.65(19.39,45.34) 0.0594 4.19(2.51,6.98) 0.3374 > 24 Hours 18 0.11(0.03,0.38) 0.6850 1.76(0.87,3.57) 0.0258 28.07(15.27,51.6) 0.2293 3.55(1.67,7.55) 0.7641 No Use 27 0.08(0.02,0.31) 0.86(0.43,1.72) 18.92(10.37,34.53) 3.13(1.49,6.6) 0-6 Hours 94 0.28(0.11,0.74) 0.4205 3.05(1.68,5.57) 0.4788 40.09(25.21,63.77) 0.9292 7.36(4.23,12.83) 0.0928 6-24 Hours 172 0.19(0.08,0.48) 0.7241 2.02(1.12,3.65) 0.0486 22.41(14.25,35.22) 0.0000 3.21(1.87,5.5) 0.0080 > 24 Hours 87 0.11(0.04,0.28) 0.0412 1.82(0.99,3.35) 0.0098 20.42(12.72,32.76) 0.0000 4.09(2.31,7.21) 0.1842 No Use 181 0.21(0.08,0.55) 2.73(1.5,4.96) 40.64(25.62,64.48) 5.28(3.04,9.17) 0-6 Hours 106 0.31(0.12,0.79) 0.0645 3.17(1.77,5.7) 0.0159 43.9(28.04,68.75) 0.1183 8.09(4.7,13.9) 0.0028 6-24 Hours 193 0.21(0.09,0.53) 0.4764 2.25(1.26,4.01) 0.9348 24.56(15.85,38.06) 0.0038 3.44(2.03,5.83) 0.0386 > 24 Hours 92 0.11(0.04,0.3) 0.1977 2.02(1.11,3.7) 0.4546 22.53(14.1,35.98) 0.0022 4(2.27,7.07) 0.3265 No Use 143 0.17(0.07,0.45) 2.27(1.26,4.1) 35.58(22.63,55.94) 4.83(2.79,8.35) 0-6 Hours 127 0.24(0.1,0.62) 0.5094 3.23(1.79,5.83) 0.0114 41.48(26.37,65.26) 0.0433 8.23(4.77,14.21) 0.0000 6-24 Hours 229 0.19(0.08,0.47) 0.9036 2.27(1.27,4.07) 0.9442 26.32(16.91,40.97) 0.1869 3.81(2.24,6.49) 0.9161 > 24 Hours 36 0.08(0.03,0.25) 0.0284 1.28(0.66,2.47) 0.0030 18.64(10.89,31.92) 0.0057 4.08(2.11,7.87) 0.7224 No Use 142 0.2(0.08,0.51) 2.25(1.24,4.09) 31.25(19.66,49.65) 3.75(2.14,6.56) soap 0-6 Hours 111 0.15(0.05,0.44) 0.0261 2.17(1.2,3.94) 0.1912 46.72(29.32,74.47) 0.0000 8.09(4.64,14.11) 0.0002 S7
eyemakeup makeup babyprod face lotion nail hair 6-24 Hours 114 0.09(0.03,0.24) 0.0000 2.23(1.25,4) 0.1822 38.94(24.82,61.08) 0.0004 5.67(3.32,9.68) 0.0166 > 24 Hours 45 0.08(0.03,0.26) 0.0007 1.6(0.84,3.02) 0.0097 22(13.1,36.93) 0.7820 2.37(1.26,4.43) 0.0899 No Use 264 0.36(0.13,0.97) 2.72(1.53,4.84) 23.25(14.98,36.1) 3.65(2.17,6.14) 0-6 Hours 66 0.45(0.17,1.18) 0.0011 2.77(1.5,5.1) 0.4409 37.11(22.71,60.65) 0.4064 6.38(3.53,11.52) 0.0786 6-24 Hours 164 0.31(0.13,0.77) 0.0070 2.35(1.3,4.24) 0.9411 25.75(16.18,40.99) 0.2203 4.26(2.45,7.42) 0.9195 > 24 Hours 51 0.37(0.13,1.04) 0.0081 1.96(1.04,3.71) 0.3724 28.39(16.85,47.84) 0.5974 5.84(3.1,10.98) 0.2106 No Use 253 0.11(0.05,0.26) 2.38(1.32,4.29) 31.72(19.96,50.42) 4.17(2.4,7.24) 0-6 Hours 83 0.34(0.13,0.92) 0.0205 2.6(1.41,4.77) 0.1445 27.4(16.47,45.58) 0.2009 6.13(3.37,11.17) 0.1319 6-24 Hours 146 0.35(0.13,0.9) 0.0133 3.07(1.7,5.57) 0.0156 22.46(13.7,36.81) 0.0198 4.56(2.56,8.13) 0.6608 > 24 Hours 30 0.55(0.17,1.83) 0.0060 3.69(1.84,7.41) 0.0167 29.27(15.86,54.03) 0.4584 5.86(2.79,12.31) 0.3049 No Use 275 0.11(0.05,0.28) 1.91(1.06,3.42) 35.95(22.21,58.18) 4.08(2.33,7.14) 0-6 Hours 44 0.11(0.04,0.32) 0.1011 3.02(1.59,5.73) 0.1904 27.86(16.41,47.28) 0.7407 4.6(2.43,8.71) 0.8443 6-24 Hours 62 0.18(0.06,0.51) 0.6551 2.91(1.57,5.39) 0.2129 34.78(21.04,57.48) 0.3797 4.99(2.74,9.08) 0.8929 > 24 Hours 47 0.15(0.04,0.53) 0.5219 1.66(0.84,3.29) 0.1614 28.4(15.84,50.92) 0.8512 2.75(1.36,5.59) 0.0692 No Use 381 0.22(0.09,0.53) 2.33(1.32,4.1) 29.73(19.16,46.14) 4.83(2.9,8.06) 0-6 Hours 105 0.19(0.07,0.48) 0.4384 2.04(1.13,3.7) 0.0771 32.52(20.6,51.33) 0.7499 5.72(3.32,9.86) 0.2764 6-24 Hours 193 0.17(0.07,0.42) 0.2626 2.15(1.2,3.84) 0.1054 24.47(15.74,38.06) 0.0300 3.81(2.26,6.43) 0.3923 > 24 Hours 57 0.13(0.05,0.36) 0.0923 2.7(1.44,5.08) 0.8776 34.58(20.87,57.29) 0.9657 6.06(3.29,11.15) 0.2002 No Use 179 0.25(0.1,0.63) 2.78(1.53,5.04) 34.31(21.69,54.29) 4.52(2.62,7.81) 0-6 Hours 129 0.28(0.11,0.71) 0.0038 3.6(2.05,6.33) 0.0000 50.55(32.23,79.27) 0.0000 8.3(4.85,14.22) 0.0000 6-24 Hours 201 0.27(0.11,0.66) 0.0030 3.24(1.86,5.64) 0.0000 35.73(23.02,55.45) 0.0000 4.82(2.86,8.13) 0.0071 > 24 Hours 53 0.23(0.08,0.64) 0.0415 2.37(1.29,4.34) 0.0002 24.99(15.13,41.29) 0.0306 3.79(2.06,6.98) 0.2121 No Use 151 0.09(0.03,0.24) 1.07(0.6,1.93) 16.07(9.97,25.89) 2.73(1.54,4.85) 0-6 Hours 3 NA NA 1.01(0.31,3.24) 0.1051 402.71(135.5,1196.4) 0.0000 62.82(15.6,251.9) 0.0001 6-24 Hours 0 NA NA NA NA NA NA NA NA > 24 Hours 0 NA NA NA NA NA NA NA NA No Use 531 0.19(0.08,0.45) 2.38(1.36,4.16) 29.59(19.21,45.57) 4.52(2.72,7.5) 0-6 Hours 57 0.15(0.06,0.41) 0.1662 2.66(1.45,4.87) 0.5399 50.4(31.09,81.68) 0.0002 8.4(4.69,15.07) 0.0036 6-24 Hours 130 0.09(0.04,0.23) 0.0009 2.12(1.18,3.83) 0.3516 27.44(17.32,43.49) 0.8160 3.5(2.01,6.09) 0.0820 S8
skinclea lip > 24 Hours 36 0.22(0.08,0.65) 0.8050 2.45(1.28,4.69) 0.9347 30.25(17.81,51.39) 0.7275 3.35(1.76,6.4) 0.1470 No Use 311 0.25(0.1,0.59) 2.41(1.37,4.27) 28.33(18.3,43.85) 4.75(2.82,8) 0-6 Hours 110 0.16(0.06,0.41) 0.4970 1.97(1.07,3.61) 0.0005 38.01(23.84,60.6) 0.0900 5.94(3.39,10.38) 0.3716 6-24 Hours 195 0.21(0.08,0.51) 0.9706 1.78(0.98,3.23) 0.0000 27.33(17.43,42.85) 0.8256 3.62(2.12,6.19) 0.1348 > 24 Hours 31 0.16(0.05,0.48) 0.5778 1.86(0.95,3.65) 0.0037 34.02(19.59,59.1) 0.4214 5.43(2.77,10.66) 0.7114 No Use 198 0.2(0.08,0.52) 3.63(1.98,6.67) 28.29(17.7,45.22) 4.88(2.78,8.56) 0-6 Hours 63 0.13(0.05,0.37) 0.2006 2.03(1.09,3.77) 0.0827 34.82(21.21,57.15) 0.3635 6.01(3.3,10.92) 0.0970 6-24 Hours 95 0.17(0.06,0.45) 0.5128 1.84(1,3.37) 0.0117 29.38(18.21,47.4) 0.9125 5.01(2.82,8.91) 0.3704 > 24 Hours 31 0.21(0.06,0.7) 0.9948 1.6(0.82,3.11) 0.0105 25.04(14.4,43.53) 0.3904 5.09(2.58,10.01) 0.4714 No Use 345 0.21(0.09,0.51) 2.72(1.53,4.83) 29.88(19.29,46.28) 4.2(2.5,7.06) * Separate models for each predictor and outcome and all models were adjusted for specific gravity. Additional covariates including study visit, time of urine collection, BMI, job category, season and number of other product categories used were also retained in the model when statistically significant. S9
Table S6: Adjusted % change of methylparaben (MP) and propylparaben (PP) breast milk concentrations according to the number of personal care product categories used in the last 24 hours. Total Personal Care Products Used (Frequency in last 24 hours) % Change MP % Change PP (95% CI) (95% CI) Low Users Reference Reference (0 4 product categories) Medium Users 100% 43% (5-7 product categories) (-84%,2442%) (-85%,1287%) High Users 260% 382% (8 14 product categories) (-75%,5014%) (-55%,5058%) *Separate models for each predictor and outcome. *The total number of product categories used in the last 24 hours by participants were summed and women were categorized into three product user groups corresponding to the bottom 43% (low users, 0 4 product categories, n=21), the middle 31% (medium users, 5-7 product categories, n=15), and top 27% (high users, 8 14 product categories, n=13) S10
Figure S1: Product Category Use by Low users (0 5 product categories), medium users (6-8 product categories), and high users (10 14 product categories) within a 24 hr period. Percentage of Users 100 90 80 70 60 50 40 30 20 10 0 24 Hour Product Category Use High Medium Low oral bodysoap shampoo skinclea lotion face condit anti eyemakeup soap hair makeup S11
Figure S2: Least squares adjusted GM by time since use (0-6h, 6-24h, >24h, No Use) S12