A Division of General Data Healthcare Histology Innovation for a NEW Generation Reagents Histology Product Reagents Name Product Here Catalog Single Source For Your Histology Reagents. Protecting Every Life Story In Your Lab. Each tissue specimen your lab processes has a life story behind it. Your mission is to ensure all your specimens receive optimal processing in order to deliver the best possible care. Our product line of histological reagents, dyes and ready-to-use stains enable your lab to deliver increased productivity, advanced specimen safety and provide highly accurate processing and staining results.
Grossing & frozen sectioning Differential Quik Stain Kit (Modified Giemsa) High quality, rapid turnaround staining kit. Useful for differential assessment of blood smears, detecting H. pylori microorganisms and immediate interpretation of fine needle aspiration biopsies. Kit contains a fixative for air-dried cell suspensions and/or touch preparation slides. Results in as little as 15 seconds! SPS-MG Differential Quik Stain Kit (Modified Giemsa); 500ml/ea TMD Tissue Marking Dyes Superior adherence to tissue that will not fade in solutions & no fixative required TMD-7 TMD-(color) For marking margins & multiple biopsies, ORANGE (O), BLACK (BK), BLUE (BL), YELLOW (Y), RED (R), PURPLE (P), GREEN (G) dyes; Includes: 2oz. of each dye/ea; 4ea/cs Individual Bottles of Marking Dyes; ORANGE (O), BLACK (BK), BLUE (BL), YELLOW (Y), RED (R), PURPLE (P), GREEN (G); 8oz/ea; 4ea/cs TFM Tissue Freezing Medium Reduced curling affords easy specimen pick up & low water content minimizes freeze fractures TFM-5 TFM-(color) Embedding matrix for frozen sections; CLEAR, RED, YELLOW, GREEN & BLUE colors; Includes: 4oz. of each color/ea; 4 ea/cs Individual Bottles of Freezing Medium; CLEAR (C), RED (R), YELLOW (Y), GREEN (G), BLUE (B) colors; 4oz/ea; 12ea/cs Mounting Media SHURMount Mounting Medium LC LC-A SHURMount Liquid cover glass for manual coverslipping, toluene-based, twist spout disposable bottle; 4oz (118ml)/ea, 12ea/cs ea/cs. SHURMount Low Viscosity Mounting medium, Xylene based for automated coverslippers; 16oz (471ml)/ea, 4 ea/cs LC-W SHURMount Mounting medium, Water based for immuno procedures; 1oz (20ml)/ea, 4 ea/cs 2 general data healthcare histology reagents product guide
microtomy / sectioning PARAGard Paraffin Repellent You ll be amazed at how well PARAGard stops paraffin from sticking to countertops and equipment, while keeping everything looking brand new. Pleasant smelling, non-toxic spray is safe for use on metal, wood and plastic surfaces. Daily cleaning chores will be reduced to a minimum. PG PARAGard, liquid paraffin repellent for countertops and equipment; 4 oz. pump bottle/ea, 6 ea/cs. PG-16 PARAGard, liquid paraffin repellent for countertops and equipment; 16 oz. spray bottle/ea, 4 ea/cs. SHURFreeze Cryogen Spray Tissue blocks can now be cooled with environmentally friendly refrigerant that meets federal specifications for reduced ozone depletion FRZ-1 SHURFreeze Cryogen spray with nozzle, 12 oz. can w/10 oz. fill; Environmentally safe, does not contain dichlorodifluoromethane; 12 ea/cs See MSDS for HAZ-MAT handling requirements Embedding Paraffin POLYFin Paraffin Wax A mixture of highly refined paraffins and co-polymers in a convenient wax pellet. This unique formulation provides optimal tissue support while maintaining exceptional clarity. POLYFin has been institutionally tested and found to function flawlessly in pressure/vacuum fluid flow processors. Low (55 C) melting point eliminates any tissue distortion caused by excessive heat during processing. - Minimal compression & cell disruption - Can safely be use in P/V processors - Ideal handling characteristics - Cut < 2 microns without icing PF POLYFin tissue infiltrating and embedding media consists of pellets of fine paraffin waxes and co-polymer alloys; 8x1 kg/cs (17.6 lbs) histology reagents product guide general data healthcare 3
fixation Acetone For hardening and dehydrating tissues. The EM grade Acetone can also be used for the extraction of various principles from animal and plant substances. A-EM16 Acetone, EM Grade, 99%, 1L/ea; See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Formalin, 10% neutral buffer (phosphate buffer) A widely used fixative for processing tissues in routine histology laboratories. The Formalin, 10% neutral buffered is for IHC in clinical laboratories for paraffin embedded tissue. F-10NB1G F-10NB5G Formalin, 10% Neutral Buffered, 1gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Formalin, 10% Neutral Buffered, 5gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Zinc Formalin Fixative, ph 6.25 Excellent morphological preservation of nuclear & cytoplasmic components. Zinc Formalin Fixative can replace neutral buffered formalin for routine tissue and immunohistochemical procedures. Best used as a primary fixative, minimizing denaturation. F-Z1G Zinc Formalin,1gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C 4 general data healthcare histology reagents product guide
tissue processing Reagent Alcohols Perfect for virtually all preparing and staining strategies in the lab. A-100RA A-95RA A-5050RA C-X1G A-I1G Reagent Alcohol, 100% SDA 3A, 95%, Isopropyl 5%; ACS/USP Grade; 1Gal/ea, 4ea/cs Reagent Alcohol, 95% SDA 3A, 95%, Isopropyl 5%; ACS/USP Grade; 1Gal/ea, 4ea/cs SHURBlend Reagent Alcohol/Isopropyl Alcohol Blend, 1Gal/ea, 4ea/cs Xylene Clearing Solvent; ACS Grade; 1gal/ea, 4ea/cs; See MSDS for HAZ-MAT handling requirements on Air Shipments Isopropanol - 99.99%, Isopropyl Alcohol; ACS/USP Grande; 1gal/ea, 4ea/cs; See MSDS for HAZ-MAT handling requirements on Air Shipments PARAClear - Xylene Substitute Removes alcohol during the final steps of routine tissue processing PARAClear Xylene Substitute is miscible with paraffin for infiltration, and it has a higher flash point than xylene, reducing the flammability danger to the laboratory. C-1G PARAClear, 1gal/ea; See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C SS-3030-120 SHURStain H&E Slide Stainer SS-2030-120 SHURStain Linear Stainer histology reagents product guide general data healthcare 5
routine stains Eosin Y, 1.0% alcoholic solution, Non-Acidic Cytoplasmic counterstain useful in immuno-histochemistry or treatment of tissue sections with hematoxylin. Contains no acetic acid. Ready-to-use with any automated stainer. E-1Y1P E-YP50GR Eosin Y, 1%, 16 oz/ea; See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Eosin Y Certified Powder, 50gm/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Gill s #2, double strength for Histology & Cytology Used when a stronger or darker nuclear stain is required for cytology or immunohistochemistry (IHC) counterstaining. Gill s #2 formulation is a double strength mixture, stains darker and more quickly than Gill s #1. General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining. H-G21L Gills 2 Hematoxylin, 1L/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at room temp. Gill s #3, triple strength for Histology Appropriate for routine histology staining where a darker nuclear stain is desired. This is a triple strength formula requiring shorter staining times. Formula is very stable and does not require filtering prior to each use. General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining. H-G31-L Gills-3 Hematoxylin, 1L/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Harris Hematoxylin, Acidified (mercury-free) Used with hematoxylin and eosin staining. Use Harris Hematoxylin for routine histology and cytology. Similar results can be achieved to those of Gill s Hematoxylin #1, #2 and #3 formulations by varying staining times. General purpose nuclear stain, regressive type. H-HAMF-1P Harris Hematoxylin,Acidified,Mercury free, 500ml/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at 25 C Lithium Blue Lithium Blue is a gentle yet rapid formulation for bluing hematoxylins in only 5 minutes. Lithium Blue is buffered at an alkalinity of ph 8.0. Our premixed and ready-to-use Lithium Blue provides crisp nuclear detail obtained with Gills, Mayer s and Harris Hematoxylin stains. For use in both cytology and histology. L-B1G Lithium Blue,Buffered,8.0ph, 1gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at room temp. Scott s Bluing Reagent Gentle bluing reagent formulation for those specimens that may be affected by more harsh agents. Our premixed and ready-to-use Scott s Bluing Reagent provides rapid bluing and crisp nuclear detail obtained with routine hematoxylin and eosin stains. For use in both cytology and histology. R-SB1G Scotts Bluing Reagent, 1gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air Shipments; Store at room temp. 6 general data healthcare histology reagents product guide
special stains AFB Ziehl Neelson Kit (Hot Method) Used to identify acid-fast organisms, mainly Mycobacteria. Helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall makes it resistant to Gram stain. Also stains other bacteria like Nocardia. Acid-fast bacilli stain bright red, non-acid fast organisms stain blue or green. SPS-ZN AFB Ziehl Neelson Kit (Hot Method). Size: 1 kit (8oz.) Acid-fast Bacilli: Bright Red Background: Blue Alcian Blue Pas Stain Kit Used for staining the acetic mucins and acid musoussubstances. SPS-ABP Alcian Blue Pas Stain Kit. Size: 500ml/ea Acidic Mucins: Blue Neutral Mucins: Magenta Mixtures of above: Blue/Purple Colloidal Iron Stain Kit A colloidal feric oxide treatment for the demostration ofacid polysaccharides. SPS-CI Colloidal Iron Stain Kit. Size: 500ml/ea Acid Mucopolysaccharides and Sialomucins: Deep Blue Nuclei: Pink-Red Cytoplasm: Pink Differential Quik Stain Kit (Modified Giemsa) High quality, rapid turnaround staining kit. Useful for differential assessment of blood smears, detecting H. pylori microorganisms and immediate interpretation of fine needle aspiration biopsies. Kit contains a fixative for air-dried cell suspensions and/or touch preparation slides. Results in as little as 10-15 seconds! SPS-MG Differential Quik Stain Kit (Modified Giemsa). Size: 500ml/ea Erythrocytes: Pink/Yellowish Red; Platelets: Dark Blue/Violet/Purple Granules; Neutrophils: Blue Nucleus, Pink Cytoplasm, Purple Granules; Eosinophils: Blue Nucleus, Blue Cytoplasm, Red Granules; Basophils: Dark Blue Purple Nucleus, Purple Granules; Monocytes: Purple Nucleus, Light Blue Cytoplasm; Bacteria/ Microorganisms: Deep Blue, vary in shape; Collagen, Cytoplasm & Muscle: Various shades of Pink, Blue, Orange & Yellow Fontana Masson Stain Kit Use in the histological visualiztion of Argentaffin cells. Also meant for use in the visualization of Melanin in paraffin or frozen sections. SPS-F Fontana Massons Stain Kit. Kit includes: 100ml/ea. Ammonium Hydroxide and 500ml/ea. of Sliver Nitrate, Gold Chloride, and Nuclear Fast Red in nalgen bottles. Argentaffin Cells: Black, Melanin: Black, Nuclei: Red, Cytoplasm: Light Pink histology reagents product guide general data healthcare 7
special stains Gram s Stain Kit (with Iodine) Used to stain microorganisms from cultures or specimens by the differential Gram method. Separates bacterial species into two large groups, those that retain the primary dye (gram-positive) and those that lose the primary dye and take the color of the secondary dye (gram-negative). The mechanism of gram stain is based on the distinctive chemistry and physical properties of the cell wall. SPS-GSI Gram s Stain Kit (with Iodine). Size: 1 kit (1 gal.) Gram-positive cells will appear Blue to Purple, while Gram-negative cells will appear Pink to Red. Masson s Trichrome Stain Kit This method is used for the detection of collagen fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections and may be used for frozen sections as well. Collagen fibers stained blue and nuclei stained black. SPS-MTS Masson s Trichrome Stain Kit. Size: 1 kit (250/500ml.) Glycogen: Red Nuclei - Blue Periodic Acid Schiff s (PAS) Stain Kit Used to demonstrate polysaccharides, neutral mucosubstances and basement membranes primarily in tissue. The demonstration of glycogen is best represented by a section of liver with a digestion step used as a negative control in the staining. Kit is supplied with Harris Hematoxylin Acidified as the counterstain. SPS-PAS Periodic Acid Schiff s (PAS) Stain Kit. Size: 1 kit (250ml.) Nuclei: Black Cytoplasm, Muscle, Erythrocytes: Red Collagen: Blue Prussian Blue Iron Stain Kit (Reaction for the Demostration of Iron) Histochemical reaction used to demonstrate ferric iron and ferritin. This is not a true staining technique. Protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian Blue. 10% NBF or alcohol can be used. Deparaffinize slides through xylene or substitute to remove paraffin from the section and descending grades of alcohol to water just prior to staining. SPS-PBI Prussian Blue Iron Stain Kit (Reaction for the Demostration of Iron. Size: 1 kit (250ml.) Iron (hemosiderin): Blue Nuclei: Red Background: Pink Verhoeff Van Gleson Elastin Stain Kit Useful in demonstrating atrophy of elastic tissue in cases of emphysema, thinning and loss of elastic fibers in arteriosclerosis and other vascular diseases. With aging, changes such as splitting and fragmentation occur, these changes are most obvious in the skin which becomes wrinkled and rather loose-fitting. SPS-GE Verhoeff Van Gleson Elastin Stain Kit. Size: 1 kit (500ml.) Elastic Fibers & Nuclei: Black Collagen: Red Other Tissue Elements: Yellow 8 general data healthcare histology reagents product guide
routine special stains Depicted below are routine stained tissue, (Bx) processed on the RTPH-360 45 minutes on the RHE (Rapid Heat Exchange) reducing processing by 2.5-3 hours. adipose 10xLMC adipose 40xLMC AFB Ziehl Neelsen bladderhe 10x bladderhe 40x breast 10xLMC breast 40xLMC breast 40xLMC-2 Colloidal Iron Stain Fontana Masson Gram10x Masson s Trichrome muscle H&E 10x musclehe 40x PAS placenta HE 10x placenta HE 40x prostate LMC 10x prostate LMC 40x Prussian Blue Iron skin 10x LMC skin 40x LMC skin HE 10x skin HE 40x stomach HE 10x stomach HE 20x stomach HE 40x thyroid HE 10x thyroid HE 40x VVG 10x skin AFB: Mycobacterium tuberculosis bacteria revealed using acid-fast Ziehl-Neelson stain; Magnified 1000X. Image courtesy of CDC Publich Health Library/Dr. George P. Kubica. E-329 Modification of Mowrey s 1958 Colloidal Iron stain (Small Instestine) for Acid Mucopolysaccharides using Newcomers control slide and Nuclear fast red counterstain. Done by Ruth Chabot 4/22/2010 histology reagents product guide general data healthcare 9
tissue processors RTP -360 Rapid Tissue Processor RTPH -360 (shown here) Rapid Tissue Processor with Rapid Heat Exchange (located behind unit) Bottles can be purchased pre-filled, or lab personnel can easily refill bottles from bulk reagent supply. Ask your local dealer for availability in your country. RTP -300 Rapid Tissue Processor RTPH -300 (shown here) Rapid Tissue Processor with Rapid Heat Exchange SP-120 Spinning Tissue Processor 10 general data healthcare histology reagents product guide
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