850 Lincoln Centre Drive Foster City, CA 94404 U.S.A. T 650.570.6667 F 650.572.2743 www.appliedbiosystems.com Dear Valued Customer: In an effort to better serve you, Applied Biosystems is revising the look and feel of our Material Safety Data Sheets, (MSDSs). In addition, we will now be supplying MSDSs for materials which are classified as nonhazardous as defined by the Dangerous Substances and Dangerous Preparations Directives, 67/548/EEC and 88/379/EEC respectively, (EU), and the OSHA Hazard Communication Standard, 29 CFR 1910.1200 (U.S.). As always, MSDSs will be available in multiple languages. In the coming months, we will be implementing a system whereby you will be able to choose your preferred method for MSDS delivery. Your delivery options are as follows: By E mail By FAX Please take a few minutes to send an e-mail to: MSDS_Inquiry_CCRM@appliedbiosystems.com to let us know your preference. Type MSDS Delivery in the subject line and include your preference in the body of the letter along with your full name, Company or Institution, and your preferred E-mail address or FAX number as applicable. Please choose only one option. Alternatively, you may print out this letter, circle your preference from the list above, and mail it to the address below. Please include your full name, Company or Institution, and E-mail address or FAX number as applicable on this letter. In the meantime, you will continue to receive your document(s) along with your order if it is the first order of the calendar year, or when the MSDS has undergone a major revision. MSDSs are also available on our website. For AB products, please visit: http://docs.appliedbiosystems.com/msdssearch.html. For Ambion products, please visit: http://www.ambion.com/techlib/msds If you are a Distributor or Dealer of Applied Biosystems Products: As the last supplier in the chain who provides hazardous substances to a place of work, you have the responsibility to provide a Material Safety Data Sheet (MSDS) and any updated hazard information from our company to your customers. You are required to send the MSDS to your downstream users prior to or with the initial delivery of a hazardous substance. When an MSDS is revised, a copy of the revised MSDS should be sent to the purchaser as soon as it is revised. Should you have any questions, feel free to contact us via email at: MSDS_Inquiry_CCRM@appliedbiosystems.com or call 650-638-5635 (USA) Thank you for your continued business. Vergine Furniss Global Chemical Compliance and Risk Management Applied Biosystems 850 Lincoln Centre Drive Foster City, CA 94404 1-800-327-3002 x5635 (USA) Revision Date: 5-Jan-07 Version 1.0
MATERIAL SAFETY DATA SHEET 1 PRODUCT AND COMPANY IDENTIFICATION Product Name: Acetic Anhydride Product No.: F9065G Trade Name(s): mrnalocator, AM1803, 1803, 9065G Manufacturer Name: Ambion Inc. 2130 Woodward Street Austin, TX, 78744 USA Phone: (512) 651-0200 www.ambion.com Emergency Telephone: 1-800-424-9300 (NORTH AMERICA); +1-703- 527-3887 (INTERNATIONAL, REVERSE CHARGES) Intended Use: For Research Use Only, Not for Use in Diagnostic Procedures 2 HAZARDS IDENTIFICATION Emergency Overview Physical State: Liquid Color: No data available. Odor: No data available. DANGER! Causes skin, eye and respiratory tract burns. Causes digestive tract burns. May be harmful if swallowed. Flammable liquid and vapor. Potential Health Effects Inhalation: Causes respiratory tract burns. May cause damage to mucous membranes in nose, throat, lungs and bronchial system. Eye Contact: Causes eye burns. Prolonged contact causes serious eye and tissue damage. Skin Contact: Causes skin burns. Ingestion: Causes digestive tract burns. May cause burns in mucous membranes, throat, esophagus and stomach. May be harmful if swallowed. NA ENGLISH MSDS 1 / 8
Chronic Health Effects: Repeated or prolonged inhalation exposure may cause asthma-like syndrome (RADS). Target Organ(s): Eye Skin Respiratory system Digestive tract Potential Physical / Chemical Effects: The product is flammable, and heating may generate vapors which may form explosive vapor/air mixtures. Water reactive material. OSHA Regulatory Status: This product is hazardous according to OSHA 29CFR 1910.1200. Environment: The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment. The product contains a substance which has a photochemical ozone creation potential. 3 COMPOSITION / INFORMATION ON INGREDIENTS General Information: The product contains: Reagent. Chemical Name CAS-No. Concentration* 108-24-7 > 60% * All concentrations are percent by weight unless ingredient is a gas. Gas concentrations are in percent by volume. This chemical is hazardous according to OSHA/WHMIS criteria. 4 FIRST AID MEASURES General: Thermal burns: Flush with water immediately. While flushing, remove clothes which do not adhere to affected area. Call an ambulance. Continue flushing during transport to hospital. Inhalation: Move injured person into fresh air and keep person calm under observation. Get medical attention immediately. Eye Contact: Flush thoroughly with water for at least 15 minutes. Get immediate medical assistance. If medical assistance is not immediately available, flush an additional 15 minutes. Make sure to remove any contact lenses from the eyes before rinsing. Skin Contact: Remove contaminated clothes and rinse skin thoroughly with water for at least 15 minutes. Chemical burns must be treated by a physician. Get medical attention immediately. Ingestion: Rinse mouth thoroughly with water and give large amounts of milk or water to people not unconscious. Do not induce vomiting. If vomiting occurs, the head should be kept low so that stomach vomit doesn't enter the lungs. Obtain medical attention and take along these instructions. 5 FIRE-FIGHTING MEASURES Extinguishing Media: Extinguish with carbon dioxide or dry powder. Unsuitable Extinguishing Media: Larger fires: Water or Foam Special Fire Fighting Procedures: Use standard firefighting procedures and consider the hazards of other involved materials. Cool containers exposed to heat with water spray and remove container, if no NA ENGLISH MSDS 2 / 8
risk is involved. Unusual Fire & Explosion Hazards: By heating and fire, corrosive vapors/gases may be formed. Solvent vapors may form explosive mixtures with air. Hazardous Combustion Products: Acetic acid, Carbon Dioxide, Carbon Monoxide Protective Measures: Selection of respiratory protection for fire fighting: follow the general fire precautions indicated in the workplace. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Flammability Class: NFPA Rating Fire = 2. 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Avoid inhalation of vapors and contact with skin and eyes. Extinguish all ignition sources. Avoid sparks, flames, heat and smoking. Ventilate. Wear suitable protective clothing. See Section 8 of the MSDS for Personal Protective Equipment. Spill Cleanup Methods: Remove sources of ignition. Absorb spillage with non-combustible, absorbent material. For waste disposal, see section 13 of the MSDS. Notification Procedures: Inform authorities if large amounts are involved. 7 HANDLING AND STORAGE Handling: Avoid inhalation of vapors and contact with skin and eyes. Mechanical ventilation or local exhaust ventilation is required. The product is flammable, and heating may generate vapors which may form explosive vapor/air mixtures. Use explosion proof electric equipment. Do not smoke and do not spray near an open flame or other sources of ignition. Ground container and transfer equipment to eliminate static electric sparks. Wear approved safety goggles. Wear protective gloves and appropriate clothing to prevent skin contact. Observe good industrial hygiene practices. Storage: Follow rules for flammable liquids. Store in closed original container in a dry place. Keep away from food, drink and animal feeding stuffs. 8 EXPOSURE CONTROLS / PERSONAL PROTECTION Exposure Limits: Chemical Name Source Type Exposure Limits Notes ACGIH TWA 5 ppm NIOSH Guide IDLH 200 ppm US. OSHA Table Z-1 TWA 5 ppm 20 mg/m³ Consult Canadian Provincial Regulations and/or Mexican Regulations on exposure limits, if applicable. Engineering Controls: Use explosion-proof ventilation equipment. Provide adequate ventilation. Observe Occupational Exposure Limits and minimize the risk of inhalation of vapors. Eye wash facilities and emergency shower must be available when handling this product. NA ENGLISH MSDS 3 / 8
Respiratory Protection: If engineering controls do not maintain airborne concentrations below recommended exposure limits (where applicable) or to an acceptable level (in countries where exposure limits have not been established), an approved respirator must be worn. In the United States of America, if respirators are used, a program should be instituted to assure compliance with OSHA Standard 63 FR 1152, January 8, 1998. Seek advice from local supervisor. Eye Protection: Wear safety glasses with side shields (or goggles) and a face shield. Hand Protection: Wear protective gloves. Be aware that the liquid may penetrate the gloves. Frequent change is advisable. Suitable gloves can be recommended by the glove supplier. Skin Protection: Wear appropriate clothing to prevent any possibility of skin contact. Protective clothing should be chemical/oil resistant. Hygiene Measures: Always observe good personal hygiene measures, such as washing after handling the material and before eating, drinking, and/or smoking. Routinely wash work clothing and protective equipment to remove contaminants. 9 PHYSICAL AND CHEMICAL PROPERTIES Color: No data available. Odor: No data available. Odor Threshold: No data available. Physical State: Liquid ph: No data available Melting Point: No data available. Freezing Point: No data available. Boiling Point: No data available. Flash Point: No data available. Evaporation Rate: No data available. Flammability Limit - Upper (%): No data available. Flammability Limit - Lower (%): No data available. Vapor Pressure: No data available. Vapor Density (Air=1): No data available. Specific Gravity: No data available. Solubility in Water: Soluble Solubility (Other): No data available. Partition Coefficient (n-octanol/water): No data available. Autoignition Temperature: No data available. Decomposition Temperature: No data available. Viscosity: No data available. 10 STABILITY AND REACTIVITY Stability: This product is stable under expected conditions of use. Conditions to Avoid: Heat, sparks, flames. Ignition sources. Water Incompatible Materials: Amines. Strong bases. Alcohols. NA ENGLISH MSDS 4 / 8
Hazardous Decomposition Products: No data available. 11 TOXICOLOGICAL INFORMATION Specified Substance(s) Acute Toxicity: Component Chemical Name Test Results Dermal LD50 (Rabbit): 4000 mg/kg Oral LD50 (Rat): 1780 mg/kg Listed Carcinogens: None. Product Information Acute Toxicity: Causes skin, eye and respiratory tract burns. Causes digestive tract burns. May be harmful if swallowed. Chronic Toxicity: Repeated or prolonged inhalation exposure may cause asthma-like syndrome (RADS). 12 ECOLOGICAL INFORMATION Ecotoxicity: The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment. Specified Substance(s) Chemical Name Test EC50 (24 hour(s), Daphnia): 55 mg/l LC50 (48 hour(s), Leuciscus idus): 265 mg/l Mobility: The product is water soluble and may spread in water systems. Persistence and Degradability: No data available. Bioaccumulation Potential: No data available. Other Adverse Effects: The product contains a substance which has a photochemical ozone creation potential. 13 DISPOSAL CONSIDERATIONS General Information: Dispose of waste and residues in accordance with local authority requirements. Disposal Methods: No specific disposal method required. Container: Since emptied containers retain product residue, follow label warnings even after container is emptied. NA ENGLISH MSDS 5 / 8
14 TRANSPORT INFORMATION DOT UN No.: UN1715 Proper Shipping Name: Class: 8 Packing Group: II Label(s): 8, 3 TDG UN No.: UN1715 Proper Shipping Name: ACETIC ANHYDRIDE Class: 8 Packing Group: II IATA UN No.: UN1715 Proper Shipping Name: Class: 8 Packing Group: II Label(s): Corrosive & Flamm. liquid IMDG UN No.: UN1715 Proper Shipping Name: ACETIC ANHYDRIDE Class: 8 Packing Group: II EmS No.: F-E, S-C 15 REGULATORY INFORMATION Canadian Controlled Products Regulations: This product has been classified according to the hazard criteria of the Canadian Controlled Products Regulations, Section 33, and the MSDS contains all required information. WHMIS Classification: B2, E Mexican Dangerous Statement: This product is dangerous according to Mexican regulations. Inventory Status: US TSCA inventory Status: All the components of this substance are listed on or are exempt from the inventory. For purposes of 40 CFR 720.36, this product is for Research and Development (R&D) Use Only. US Regulations CERCLA Hazardous Substance List (40 CFR 302.4): Chemical Name RQ 5000 lbs SARA Title III NA ENGLISH MSDS 6 / 8
Section 302 Extremely Hazardous Substances (40 CFR 355, Appendix A): Not regulated. MSDS PN: 4396438 Section 311/312 (40 CFR 370): X Acute (Immediate) X Chronic (Delayed) X Fire X Reactive Pressure Generating Section 313 Toxic Release Inventory (40 CFR 372): Not regulated. Clean Air Act (CAA) Section 112(r) Accidental Release Prevention (40 CFR 68.130): Not regulated. Clean Water Act Section 311 Hazardous Substances (40 CFR 117.3): Drug Enforcement Act: Drug Enforcement Administration (DEA). List 2, Essential Chemicals (21 CFR 1310.02(b) and 1310.04(f)(2)): TSCA: TSCA Section 4(a) Final Test Rules & Testing Consent Orders: Not regulated. TSCA Section 5(a)(2) Final Significant New Use Rules (SNURs) (40CFR 721, Subpt. E): Not regulated. TSCA Section 5(e) PMN-Substance Consent Orders: Not regulated. TSCA Section 12(b) Export Notification (40 CFR 707, Subpt. D): Not regulated. State Regulations California Safe Drinking Water and Toxic Enforcement Act of 1986 (Proposition 65): Not regulated. Massachusetts Right-To-Know List: Michigan Critical Materials List (Michigan Natural Resources and Environmental Protection Act (Act. 451 of 1994)): Not regulated. Minnesota Hazardous Substances List: New Jersey Right-To-Know List: Pennsylvania Right-To-Know List: Rhode Island Right-To-Know List: 16 OTHER INFORMATION HAZARD RATINGS Health Hazard Fire Hazard Instability Special Hazard NA ENGLISH MSDS 7 / 8
NFPA 3 2 1 -- Hazard rating: 0 - Minimal; 1 - Slight; 2 - Moderate; 3 - Serious; 4 - Severe NFPA Label colored diamond code: Blue - Health; Red - Flammability; Yellow - Instability; White - Special Hazards MSDS PN: 4396438 Health Hazard Flammability Physical Hazard Personal Protection HMIS 3* 2 1 B Hazard rating: 0 - Minimal; 1 - Slight; 2 - Moderate; 3 - Serious; 4 - Severe *- Chronic Health Effect Personal Protection codes: B - Safety Glasses, Gloves HMIS Label colored bar code: Blue - Health; Red - Flammability; Orange - Physical Hazards; White - Special Disclaimer: This MSDS has been prepared using information from sources considered technically reliable. It should not be relied upon as a product specification. The company makes no warranty of any kind, express or implied, concerning the safe use of this material in your process or in combination with other chemical substances. Every user of the product is responsible for evaluating its own processes and conditions of use and selecting appropriate protective measures for employee exposure. NA ENGLISH MSDS 8 / 8