Kewpie s 3 brothers of functional HA
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1 Kewpie s 3 brothers of functional HA 1
2 Hyaloveil-P Adhesion type HA for skin/hare-care
3 Introduction 3/15 Every day I use expensive cosmetics which contain HA. But I m afraid HA is washed away when I wash my face at night. What a waste!
4 Mechanism of ADHESION to the skin 1 Skin s surface is normally electrically charged negative. 2 While Hyaloveil -P is charged positive. X Ⅹ:Na or n CH 2 CHCH 2 N + CH 3 OH CH 3 CH 3 Cl- Chemical structure of Hyaloveil -P Hydroxypropyltrimonium 3 Hyaloveil -P is considered to adhere to the skin by means of ionic bond. R
5 Adhesion test of Hyaloveil R -P to the skin Test sample 1 1% solution of Hyaloveil R -P 2 1% solution of common hyaluronic acid <test method> Applying the test sample to the subject s forearm. Leaving it at rest for 3 minutes. Washing the sample off the forearm by running water. Sticking and stripping adhesive tape Flow-chart of the test Measuring the residue of hyaluronic acid in the adhesive tape by HPLC analysis R
6 Adhesion test of Hyaloveil R -P to the skin Residue on the skin(μg) <Test result> Hyaloveil -P P<0.05 Hyaluronic Acid Residue of Hyaloveil R -P and Hyaluronic acid on the skin (Average of 15 objects) It was confirmed that Hyaloveil R -P was not washed off but remained on the skin. R
7 Skin s moisture retention test with Hyaloveil R -P <Test method> Test sample 1 1% solution of Hyaloveil R -P 2 1% solution of common Hyaluronic acid Applying the test sample to the subject s forearm. (n=12) Leaving it at rest for 3 minutes. Washing the sample off the forearm by running water. Removing water from the surface of the skin. Flow-chart of the test Measuring moisture contents by time (after 5 min. 10 min. 15 min.) using skin surface stratum corneum moisture meter(skicon-200) R
8 Skin s moisture retention test with Hyaloveil R -P moisture increase (μs) <Test result> 20 after 5 min. * 20 After 10 min. 20 After 15 min * * without Hyaloveil -P 1% solution Hyaluronic acid 1% solution -5 *: Significant differences between Hyaloveil -P and the other two groups were confirmed by t-test (p<0.01) It was confirmed that Hyaloveil R -P was not washed off but remained on the skin and moistened it. R
9 Electron Micrograph <Method of the test> Hyaloveil -P 1% solution Damaged Hair (Bleached Hair) Soaking (40 5minutes) Rinsing with water Drying Observation with Scanning Electron Microscope (SEM) R
10 Hyaloveil-P attaches the hair and make the surface smoother Untreated Hair Treated Hair with Hyaloveil -P
11 Hyaloveil-P improves the hair smoothness (water solution) 1 Water (Blank) <Samples> 2 Hyaloveil -P 0.1% water solution <Procedure> Sample12(160g each) hair after brushing (9g each) 40 20minutes Wash the hair (shake in the 2L of water) Dry with towel Dry with dryer Brushing Leave a room temperature Test with tester
12 Average friction coefficient Hyaloveil-P improves the hair smoothness (water solution) * *:P<0.05 Water (Blank) Hyaloveil -P 0.1% solution
13 Hyaloveil-P improves the hair smoothness (conditioner) <Samples> 1 Conditioner containing no Hyaloveil-P(Blank) 2 Conditioner containing 0.5% of Hyaloveil-P 3 Conditioner containing 0.8% of Hyaloveil-P <Procedure> Check the easy-to-comb property, during washing and after washing, by using normal hair and Dynamic Combing Tester
14 Hyaloveil-P improves the hair smoothness (conditioner) <Easy-to-comb property> *P<0.05 During washing After washing * * * 0% 1 0.5% 2 0.8% 3 Hyaloveil-P 0% 1 0.5% 2 0.8% 3 Hyaloveil-P
15 Hyaloveil-P improves shampoo foaming <Samples> 1 Shampoo with no Hyaloveil-P (Blank) 2 Shampoo containing 0.5% of Hyaloveil-P 3 Shampoo containing 0.8% of Hyaloveil-P <Test method>(blender Foam Volume Drainage method) 1. Add water and liquid lanolin into each sample, and mix, then add 150ppm of hard water(40 ). 2. Mix, 10seconds 3. Transfer to measuring cylinder, and measure total volume of foam and liquid 4. Repeat the measurement 3 times, from the average, give a score as maximum of 100.
16 Hyaloveil-P improves shampoo foaming *P<0.05 * * Blank 0.5% Hyaloveil-P 0.8% Hyaloveil-P
17 How Hyaloveil-P works on the skin/hair Partially cationized Hyaloveil-P attaches to skin and hair surface with moisture Make the surface smoother, and give a good touch It can also attach to the scalp with moisture, and might help to grow hair
18 Big trend of silicon-free hair care Hyaloveil-P Adhesion Test to the hair Hyaloveil-P Volume adhered to 1 gram of hair (mg) (Zero) Hyaluronic Acid Hyaloveil-P ヒアルロン酸ヒアロベール -P Untreated Hair Treated Hair with Hyaloveil -P
19 Kewpie s 3 brothers of functional HA 19
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