Safety Data Sheet F2/F5/MTHFR Extraction Tray. 1. Identification
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1 1. Identification 1.1. Product identifier Product Identity F2/F5/MTHFR (HC) Extraction Tray Alternate Names Verigene Verigene F2/F5/MTHFR Nucleic Acid Test Kit 1.2. Relevant identified uses of the substance or mixture and uses advised against Intended use See Technical Data Sheet. Application Method See Technical Data Sheet Details of the supplier of the safety data sheet Company Name Nanosphere, Inc 4088 Commercial Ave. Northbrook, IL Contact Information PHONE/FAX: (847) / (847) address : technicalsupport@nanosphere.us Emergency Telephone Number CHEMTREC- 24 Hr.Info Within USA and Canada: Outside USA and Canada: (collect calls accepted) In Country/Regional Emergency Contact : South Africa : Argentina (Buenos Aires) : +(54) Brazil (Rio De Janeiro) : +(55) Chile (Santiago) : +(56) Colombia : Mexico : Peru (Lima) : +(51) Bahrain : +(973) China : Hong Kong : India : Israel (Tel Aviv) : +(972) Japan (Tokyo) : Russia : Saudi Arabia : +(966) Australia (Sydney) : +(61) New Zealand (Auckland) : +(64) Austria (Vienna) : +(43) Belgium (Brussels) : +(32) Bulgaria (Plovdiv) : +(359) France : +(33) Germany : Germany (Frankfurt) : +(49) Ireland (Dublin) : +(353) Document MFLBL-023 CR Number: CR Implemented: MAR Page 1 of 13
2 Italy : Luxembourg : +(352) Netherlands : +(31) Poland (Warsaw) : +(48) Portugal : +(351) London : +(44) Hazard(s) identification 2.1. Classification of the substance or mixture Flam. Liq. 3;H226 Acute Tox. 5;H303 Skin Irrit. 2;H315 Eye Irrit. 2;H319 Repr. 1B;H360D Acute Tox Oral Cat 4; H302 Acute Tox Inhalation Cat 4; H332 Eye Damage Cat 1; H318 Skin Irrit. Cat 2; H315 Resp. Sens. Cat 1; H334 Repro Cat 1B; H360D Aquatic Chronic Tox. Cat 2; H411 Flam. Liq. Cat 3; H226 Flammable liquid and vapor. May be harmful if swallowed. (Not adopted by US OSHA) Causes skin irritation. Causes serious eye irritation. May damage the unborn child Label elements Using the Toxicity Data listed in section 11 and 12 the product is labeled as follows. H226 Flammable liquid and vapor. H303 May be harmful if swallowed. H315 Causes skin irritation. H319 Causes serious eye irritation. H360D May damage the unborn child. Danger Document MFLBL-023 CR Number: CR Implemented: MAR Page 2 of 13
3 H318 H334 H360D EUH032 [Prevention]: P201 Obtain special instructions before use. P280 Wear protective gloves / eye protection / face protection. P281 P273 [Response]: P IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P310 P IF exposed or concerned: Get medical advice / attention. P304+P341 P342+P311 [Storage]: [Disposal]: 3. Composition/information on ingredients This product contains the following substances that present a hazard within the meaning of the relevant State and Federal Hazardous Substances regulations. Solution 1 Thiocyanic acid, compound with guanidine (1:1) CAS Number: Poly(oxy-1,2-ethanediyl),.alpha.-[4-(1,1,3,3- tetramethylbutyl)phenyl]-.omega.-hydroxy- CAS Number: Acute Tox. 4;H302 Acute Tox. 4;H312 Acute Tox. 4;H332 Aquatic Chronic 3;H Acute Tox. 4;H302 Skin Irrit. 2;H315 Eye Dam. 1;H318 Aquatic Chronic 2;H411 [1] [1] Solution 2 Formamide Repr. 1B;H360D* [1][2] Document MFLBL-023 CR Number: CR Implemented: MAR Page 3 of 13
4 CAS Number: Nitric acid sodium salt CAS Number: Eye Irrit. 2;H319 Ox. Sol. 3;H272 [1] Solution 3 Glycerol CAS Number: Not Classified [1][2] Proteinase, tritirachium album serine CAS Number: Skin Irrit. 2;H315 Eye Irrit. 2;H319 Resp. Sens. 1;H334 [1] Solution 4 Guanidine, monohydrochloride CAS Number: Ethanol CAS Number: Acute Tox. 4;H302 Eye Irrit. 2;H319 Skin Irrit. 2;H315 [1] Flam. Liq. 2;H225 [1][2] Solution 5 Guanidine, monohydrochloride Acute Tox. 4;H302 [1] CAS Number: Eye Irrit. 2;H319 Skin Irrit. 2;H315 Ethanol Flam. Liq. 2;H225 [1][2] CAS Number: Solution 6 Ethanol CAS Number: Flam. Liq. 2;H225 [1][2] Solution 7 There are no ingredients in this product which are classified as hazardous, and/or no hazardous ingredients above the GHS cut off percentage. Solution 8 There are no ingredients in this product which are classified as hazardous, and/or no hazardous ingredients above the GHS cut off percentage. Solution 9 Document MFLBL-023 CR Number: CR Implemented: MAR Page 4 of 13
5 There are no ingredients in this product which are classified as hazardous, and/or no hazardous ingredients above the GHS cut off percentage. Solution 10 Isopropyl Alcohol CAS Number: Flam. Liq. 2;H225 Eye Irrit. 2;H319 STOT SE 3;H336 [1][2] [1] Substance classified with a health or environmental hazard. [2] Substance with a workplace exposure limit. [3] PBT-substance or vpvb-substance. *The full texts of the phrases are shown in Section First aid measures 4.1. Description of first aid measures General In all cases of doubt, or when symptoms persist, seek medical attention. Never give anything by mouth to an unconscious person. Inhalation Remove to fresh air, keep patient warm and at rest. If breathing is irregular or stopped, give artificial respiration. If unconscious place in the recovery position and obtain immediate medical attention. Give nothing by mouth. Eyes Irrigate copiously with clean water for at least 15 minutes, holding the eyelids apart and seek medical attention. Skin Remove contaminated clothing. Wash skin thoroughly with soap and water or use a recognized skin cleanser. Ingestion If swallowed obtain immediate medical attention. Keep at rest. Do NOT induce vomiting Most important symptoms and effects, both acute and delayed Overview Exposure to solvent vapor concentrations from the component solvents in excess of the stated occupational exposure limits may result in adverse health effects such as mucous membrane and respiratory system irritation and adverse effects on the kidneys, liver and central nervous system. Symptoms include headache, nausea, dizziness, fatigue, muscular weakness, drowsiness and in extreme cases, loss of consciousness. Eyes Skin Ingestion Repeated or prolonged contact with the preparation may cause removal of natural fat from the skin resulting in dryness, irritation and possible non-allergic contact dermatitis. Solvents may also be absorbed through the skin. Splashes of liquid in the eyes may cause irritation and soreness with possible reversible damage. See section 2 for further details. Causes serious eye irritation. Causes skin irritation. May be harmful if swallowed. (Not adopted by US OSHA) Document MFLBL-023 CR Number: CR Implemented: MAR Page 5 of 13
6 5. Fire-fighting measures 5.1. Extinguishing media Use media appropriate for surrounding area Special hazards arising from the substance or mixture Hazardous decomposition: Oxides of phosphorus, oxides of sulfur, phosphine. Keep away from heat / sparks / open flames / hot surfaces - No smoking. Keep cool. Ground / bond container and receiving equipment. Use explosion-proof electrical / ventilating / light / equipment. Use only non-sparking tools. Take precautionary measures against static discharge Advice for fire-fighters None ERG Guide No Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Put on appropriate personal protective equipment (see section 8) Environmental precautions Use good personal hygiene practices. Wash hands before eating, drinking, smoking or using toilet. Promptly remove soiled clothing and wash thoroughly before reuse Methods and material for containment and cleaning up Ventilate the area and avoid breathing vapors. Take the personal protective measures listed in section 8. Contain and absorb spillage with non-combustible materials e.g. sand, earth, and vermiculite. Place in closed containers outside buildings and dispose of according to the Waste Regulations. (See section 13). Clean, preferably with a detergent. Do not use solvents. 7. Handling and storage 7.1. Precautions for safe handling The requirements of the Highly Flammable Liquids and Liquefied Petroleum Gases Regulations apply if the flashpoint is between 21 C and 32 C. See section 2 for further details. - [Prevention]: 7.2. Conditions for safe storage, including any incompatibilities Document MFLBL-023 CR Number: CR Implemented: MAR Page 6 of 13
7 Handle containers carefully to prevent damage and spillage. Naked flames and smoking should not be permitted in storage areas. It is recommended that fork lift trucks and electrical equipment are protected to the appropriate standard. Incompatible materials: Strong oxidizing agents, strong bases See section 2 for further details. - [Storage]: 7.3. Specific end use(s). 8. Exposure controls and personal protection 8.1. Control parameters Exposure CAS No. Ingredient Source Value Glycerol OSHA TWA 15 mg/m3 (total) TWA 5 mg/m3 (resp) ACGIH TWA: 3 mg/m3 (respirable) 10 mg/m3 (mist) NIOSH No established RELs Supplier No Established Limit Ethanol OSHA TWA 1000 ppm (1900 mg/m3) ACGIH STEL: 1000 ppm Revised 2009, NIOSH TWA 1000 ppm (1900 mg/m3) Supplier No Established Limit Isopropyl Alcohol OSHA TWA 400 ppm (980 mg/m3)stel 500 ppm ACGIH TWA: 200 ppm STEL: 400 ppm Revised 2003, NIOSH TWA 400 ppm (980 mg/m3) ST 500 ppm (1225 mg/m3) Supplier No Established Limit Formamide OSHA No Established Limit ACGIH TWA: 10 ppms NIOSH TWA 10 ppm (15 mg/m3) [skin] Supplier No Established Limit Carcinogen Data CAS No. Ingredient Source Value Ethanol OSHA Select Carcinogen: No NTP Known: No; Suspected: No IARC Group 1: Yes; Group 2a: No; Group 2b: No; Group 3: No; Group 4: No; Isopropyl Alcohol OSHA Select Carcinogen: No NTP Known: No; Suspected: No IARC Group 1: No; Group 2a: No; Group 2b: No; Group 3: Yes; Group 4: No; Document MFLBL-023 CR Number: CR Implemented: MAR Page 7 of 13
8 8.2. Exposure controls Respiratory None required when used as intended. Eyes Protective safety glasses recommended Skin Protective gloves recommended Engineering Controls None normally required Other Work Practices Use good personal hygiene practices. Wash hands before eating, drinking, smoking or using toilet. Promptly remove soiled clothing and wash thoroughly before reuse. See section 2 for further details. - [Prevention]: 9. Physical and chemical properties Appearance Odor Odor threshold ph Melting point / freezing point Initial boiling point and boiling range Flash Point Evaporation rate (Ether = 1) Flammability (solid, gas) Upper/lower flammability or explosive limits Vapor pressure (Pa) Vapor Density Specific Gravity Solubility in Water Partition coefficient n-octanol/water (Log Kow) Auto-ignition temperature Decomposition temperature Viscosity (cst) 9.2. Other information No other relevant information. Liquid Not measured 95 F Not Applicable Lower Explosive Limit: Upper Explosive Limit: 10. Stability and reactivity Reactivity Hazardous Polymerization will not occur Chemical stability Document MFLBL-023 CR Number: CR Implemented: MAR Page 8 of 13
9 Stable under normal circumstances Possibility of hazardous reactions Conditions to avoid Keep away from oxidizing agents, excessive heat, and ignition sources Incompatible materials Strong oxidizing agents, strong bases Hazardous decomposition products Oxides of phosphorus, oxides of sulfur, phosphine. 11. Toxicological information Acute toxicity Exposure to solvent vapor concentrations from the component solvents in excess of the stated occupational exposure limits may result in adverse health effects such as mucous membrane and respiratory system irritation and adverse effects on the kidneys, liver and central nervous system. Symptoms include headache, nausea, dizziness, fatigue, muscular weakness, drowsiness and in extreme cases, loss of consciousness. Repeated or prolonged contact with the preparation may cause removal of natural fat from the skin resulting in dryness, irritation and possible non-allergic contact dermatitis. Solvents may also be absorbed through the skin. Splashes of liquid in the eyes may cause irritation and soreness with possible reversible damage. Ingredient Oral LD50, mg/kg Skin LD50, mg/kg Inhalation Vapor LC50, mg/l/4hr Inhalation Dust/Mist LC50, mg/l/4hr Inhalation Gas LC50, ppm Isopropyl Alcohol - ( ) 4,710.00, Rat - Category: 5 12,800.00, Rat , Rat - Ethanol - ( ) 7,060.00, Rat - 20,000.00, Rabbit , Rat - Glycerol - ( ) 12,600.00, Rat - 10,000.00, Rabbit - Note: When no route specific LD50 data is for an acute toxin, the converted acute toxicity point estimate was used in the calculation of the product's ATE (Acute Toxicity Estimate). Classification Category Hazard Description Acute toxicity (oral) 5 May be harmful if swallowed. (Not adopted by US OSHA) Acute toxicity (dermal) --- Not Applicable Acute toxicity (inhalation) --- Not Applicable Document MFLBL-023 CR Number: CR Implemented: MAR Page 9 of 13
10 Skin corrosion/irritation 2 Causes skin irritation. Serious eye damage/irritation 2 Causes serious eye irritation. Respiratory sensitization --- Not Applicable Skin sensitization --- Not Applicable Germ cell mutagenicity --- Not Applicable Carcinogenicity --- Not Applicable Reproductive toxicity 1B May damage the unborn child. STOT-single exposure --- Not Applicable STOT-repeated exposure --- Not Applicable Aspiration hazard --- Not Applicable 12. Ecological information Toxicity The preparation has been assessed following the conventional method of the Dangerous Preparations Directive 1999/45/EC and GHS and is not classified as dangerous for the environment, but contains substance(s) dangerous for the environment. See section 3 for details Aquatic Ecotoxicity Ingredient Isopropyl Alcohol - ( ) 96 hr LC50 fish, mg/l 1,400.00, Lepomis macrochirus 48 hr EC50 crustacea, mg/l ErC50 algae, mg/l , Daphnia magna (72 hr), Scenedesmus subspicatus Formamide - ( ) Not Available Not Available Not Available Ethanol - ( ) 42.00, Oncorhynchus mykiss 2.00, Daphnia magna (96 hr), Ulva pertusa Guanidine, monohydrochloride - ( ) Not Available Not Available Not Available Glycerol - ( ) 67,500.00, Oncorhynchus mykiss 10,000.00, Daphnia magna Not Available Persistence and degradability There is no data on the preparation itself Bioaccumulative potential Mobility in soil Results of PBT and vpvb assessment This product contains no PBT/vPvB chemicals Other adverse effects. Document MFLBL-023 CR Number: CR Implemented: MAR Page 10 of 13
11 13. Disposal considerations Waste treatment methods Observe all federal, state and local regulations when disposing of this substance. 14. Transport information DOT (Domestic Surface Transportation) IMO / IMDG (Ocean Transportation) ICAO/IATA UN number Not Applicable Not Regulated Not Regulated UN proper shipping Not Regulated Not Regulated Not Regulated name Transport hazard DOT Hazard Class: Not IMDG: Not Applicable Air Class: Not Applicable class(es) Applicable Sub Class: Not Applicable Packing group Not Applicable Not Applicable Not Applicable Environmental hazards IMDG Marine Pollutant: No Special precautions for user No further information 15. Regulatory information Regulatory Overview Toxic Substance Control Act ( TSCA) WHMIS Classification US EPA Tier II Hazards The regulatory data in Section 15 is not intended to be all-inclusive, only selected regulations are represented. All components of this material are either listed or exempt from listing on the TSCA Inventory. B2 D2A Fire: Yes Sudden Release of Pressure: No Reactive: No Immediate (Acute): Yes Delayed (Chronic): No EPCRA 311/312 Chemicals and RQs: To the best of our knowledge, there are no chemicals at levels which require reporting under this statute. EPCRA 302 Extremely Hazardous: To the best of our knowledge, there are no chemicals at levels which require reporting under this statute. Document MFLBL-023 CR Number: CR Implemented: MAR Page 11 of 13
12 EPCRA 313 Toxic Chemicals: Isopropyl Alcohol Nitric acid sodium salt Proposition 65 - Carcinogens (>0.0%): To the best of our knowledge, there are no chemicals at levels which require reporting under this statute. Proposition 65 - Developmental Toxins (>0.0%): To the best of our knowledge, there are no chemicals at levels which require reporting under this statute. Proposition 65 - Female Repro Toxins (>0.0%): To the best of our knowledge, there are no chemicals at levels which require reporting under this statute. Proposition 65 - Male Repro Toxins (>0.0%): To the best of our knowledge, there are no chemicals at levels which require reporting under this statute. New Jersey RTK Substances (>1%): Ethanol Formamide Glycerol Isopropyl Alcohol Pennsylvania RTK Substances (>1%): Ethanol Formamide Glycerol Isopropyl Alcohol 16. Other information The information and recommendations contained herein are based upon data believed to be correct. However, no guarantee or warranty of any kind, expressed or implied, is made with respect to the information contained herein. We accept no responsibility and disclaim all liability for any harmful effects which may be caused by exposure to our products. Customers/users of this product must comply with all applicable health and safety laws, regulations, and orders. The full text of the phrases appearing in section 3 is: H225 Highly flammable liquid and vapor. H302 Harmful if swallowed. H312 Harmful in contact with skin. H315 Causes skin irritation. H318 Causes serious eye damage. H319 Causes serious eye irritation. H332 Harmful if inhaled. H334 May cause allergic or asthmatic symptoms or breathing difficulties if inhaled. Document MFLBL-023 CR Number: CR Implemented: MAR Page 12 of 13
13 H336 May cause drowsiness and dizziness. H360D May damage the unborn child. H411 Toxic to aquatic life with long lasting effects. H412 Harmful to aquatic life with long lasting effects. This is the first version in the GHS SDS format. Listings of changes from previous versions in other formats are not applicable. The information provided on this MSDS is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. It remains the user s own responsibility to make sure that the information is appropriate and complete for his specific use of this product. The user is also responsible for observing any laws and applicable guidelines. End of Document Document MFLBL-023 CR Number: CR Implemented: MAR Page 13 of 13
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