Patented BCX-CA. (Natural anti-acne treatment)
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1 Patented BCX-CA (Natural anti-acne treatment)
2 Product introduction BCX-CA (Natural anti-acne treatment) 1. A n t i - a c n e t r e a t m e n t t h a t m a n u f a c t u r e d w i t h n a t u r a l i n g r e d i e n t s ( c o n s i s t o f C h a m a e c y p a r i s o b t u s a, G l y c y r r h i z a u r a l e n s i s, V i t i s b i n i f e r a s e e d o i l, C i t r u s u n s h i u F r u i t E x t r a c t, L a c t o b a c i l l u s F e r m e n t ) 2. B i o c o n v e r s i o n v i a f e r m e n t a t i o n i s u s e d 3. W i t h b e t t e r a n t i - a c n e e f f e c t t h a n Te a t r e a o i l ( I n v i t r o & I n v i v o t e s t ) 4. G o t p u b l i c a t i o n a n d p a t e n t ( D e r m a t o l o g y, Vo l ( 2 ) , ) 5. W a t e r - s o l u b l e. C o u l d a p p l y t o m o r e p r o d u c t s t h a n Te a t r e a o i l Anti-acne Anti-inflammatory Reduce sebum 6. U s e d i n P u r e c o s p r o d u c t s, e x p o r t t o T h a i l a n d, p r o c e s s i n g e x p e r i m e n t s i n L O R E A L 2
3 Ingredients INCI Name Chamaecyparis obtusa Leaf Extract INCI Name Citrus unshiu Fruit Extract INCI Name Glycyrrhiza glabra Root Extract INCI Name Vitis vinifera Seed Oil INCI Name Lactobacillus Ferment 3
4 Anti-bacterial experiments compare with Tea tree oil Product Inhibition Zone* (mm) MIC (% v/v) 1 BCX-CA BCX-A Tea tree oil BCX-A 2. BCX-CA 3. TTO BCX-CA got the best anti-acne effect than BCX-A and Tea tree oil 4
5 Experiment of anti-acne activity Experiments of P. Acnes growth inhibition (Incubated for 24hr) Tea tree oil 5% BCX-CA 5% (After bioconversion) Anti-acne experiment KTR* experiment Anti-bacterial experiment [Halo test : P. acnes, for 72hr] BCX-CA 5% (Before bioconversion) Control group BCX-CA Salicylic acid 5% * Korea Testing and Research Institute 5
6 Anti-bacterial experiment effect of bioconversion The effect of BCX-CA before and after bioconversion <Dermatology, Vol. 229(2) , 2014> Before After The content of three substances is increased after bioconversion 6
7 <Dermatology, Vol. 229(2) , 2014> Effect of anti-inflammation and sebum-generation BCX-CA Tea tree oil Inflammation Non-inflammation Inflammation Non-inflammation 1. The proteins related to inflammation is much more decreased by BCX-CA than Tea tree oil. (NFκB, TLR2, IL8, IL1α : Inflammatory cytokine gene products) 2. BCX-CA decreases the level of SREBP-1 and IGF-1R which is not observed in Tea tree oil group. (Not related to inflammation, SREBP1 and IGF1R are related to the generation of sebum) * BCX-CA activates the receptors that activate the generation of sebum via SREBP1 7
8 Cytotoxicity MTT Assay (Cell line: Hs68) * p<0.05 The proliferation rate is more than 78% with 10% BCX-CA BCX-CA is not cytotoxic 8
9 Solubility Compare with Tea Tree Oil BCX-CA is Water-soluble!!! could be applied to various products BCX-CA Tea tree oil
10 Stability/Viscosity BCX-CA (Natural anti-acne treatment) BCX-CA 4 o C (1month) 25 o C (1month) 45 o C (1month) Precipitation None None None Bio-activity Not changed Not changed Not changed at 45 o C 2. at room temperature
11 Human patch experiment Period of experiment: 2013/10/17 ~ 2013/10/20 Purpose of experiment : To observe the stability of BCX-CA while mixed with cosmetics Results Group 1hr 24hr Judgment - ± ± + ++ Control No irritation BCX-CA 50% No irritation * Control : Lotion base # Standard: - Negative ± Doubtable reaction + Mild reaction (red spot, papule) ++ Strong reaction (red spot, papule, blister) No irritation was observed with BCX-CA treatment The stability of BCX-CA is approved!
12 Clinical experiment processed in Seoul National University Hospital Dermatology, Vol. 229(2) , 2014
13 Clinical experiment Experimental design - Randomized blind test was performed for 8 weeks - The experiment was performed during 2013/ 1~2013/ 6 in Seoul National University Hospital - BCX-CA and Tea tree oil were used in this experiment
14 Clinical experiment Results <Dermatology, Vol. 229(2) , 2014> BCX-CA: 5% Lactobacillus fermented Chamaecypris obtuse Tea tree oil: 5% Tea tree oil BCX-CA Tea tree oil BCX-CA Tea tree oil BCX-CA could get the skin recovered at both inflammatory and non-inflammatory lesions (The effect in inflammatory lesions is better than in non-inflammatory lesions!)
15 Clinical experiment Results BCX-CA: 5% Lactobacillus fermented Chamaecypris obtuse Tea tree oil: 5% Tea tree oil <Dermatology, Vol. 229(2) , 2014> Before BCX-CA Tea tree oil 8 weeks
16 Application BCX-CA Cream INCI (%) Purified water Glycerin 2.00 A BCX-CA 5.00 Niacinamide ,3-Butylene Glycol 2.00 PEG-60 Hydrogenated Castor Oil 0.30 B Glyceryl MonoStearate; PE -100 Stearate 0.80 Cetyl Ethylhexanoate 5.00 C Purified water Sodium polyacrylate 0.40 Purified water 4.00 D Silk amino acid 0.10 Azulene 0.10 Hydrolysed Collagen 0.10 E Fragence 0.50 Citrus peel oil 0.50 F Polyacrylamide& C13-14 Isoparaffin& Laurenth Total
17 Certification/Patent Patented
18 Directions Appearance Brown liquid ph 6.7±0.5 Recommend dosage Solubility Storage Notice Package 2~5% Water-soluble Store at dry and cool place. Away from direct sun light. - Add into liquid - Xanthan Gum is recommended to be used as thickener (The viscosity might change with carbomer) - It is fin to adjust ph value 10kg & 20kg
19 Thank you WEB TEL FAX B-Line B101, 14, Sagimakgolro 45beon-gil, Jungwon-gu, Seongnam-si, Gyeonggi-do, Republic of Korea 19
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