Hylasome EG10. Excellent Moisturizing Through the Delivery of Water
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1 ylasome EG10 Excellent Moisturizing Through the Delivery of Water ylasome is a registered trademark of Genzyme Corpora on. US Patents 5,143,724; 5,246,698; 5,399,351; other patent pending
2 ylasome is the tradename of ylan B gel (cross- linked hyaluronic acid) used in topical applica ons. ylamed Research, Inc. has the exclusive, global license for ylasome technology for use in the topical cosme c and skin care field. Lipo Chemicals is the exclusive worldwide distributor for ylamed Research.
3 Medical Products containing ylan B gel: Synvisc : treatment for osteoarthri s; injec on of Synvisc (ylan B gel/ylan A A mixture) into the knee relieves the pain of osteoarthri s. ylaform : so ssue augmenta on- correc on of facial wrinkles and scars by intradermal injec on of ylaform (composed of ylan B gel)
4 INCI name: Sodium yaluronate Crosspolymer CAS No.: EINECS No.: Polymer, Exempt Consists of an aqueous gel of crosslinked hyaluronic acid. Polymer content: 6% by weight of polymer plus bound water
5 Chemical Structure C - N C 3 N C - C 3 S S C 3 C - C - N N C 3 Sulfonyl- bis- ethyl crosslinks and hydroxyethyl sulfonyl ethyl pendant groups
6 Crosslinking
7 Delivery of Water Unique non- equilibrium gel. Developed specifically in a non- equilibrium state with gel domains that are not completely swollen. Gel domains, which contain ghtly bound water, are surrounded by free water. The gel forms a smooth film on the skin and con nuously delivers the bound water.
8
9 Skin Moisturiza on Study: Confocal Raman Spectroscopy Ex vivo surgically removed skin (including stratum corneum, viable epidermis, and some dermis). ylasome EG10 vs. yaluronic Acid, both at 0.1% polymer weight in D 2 Performed at Rutgers University
10 Experimental Procedure 5 µl D 2 - based test material applied to 2x2 cm 2 surgically removed skin. eld at 55% rela ve humidity (D 2 ) and room temperature for 24 hours. Placed in milled brass sample holder and covered with glass cover slip. D 2 content in the skin measured by confocal Raman spectroscopy in the x and z axes.
11 Confocal Raman Spectoscopy Confocal sampling skin sec ons parallel to surface placed in a brass block (SC up) sealed with cover slip 785 nm in Raman out 785 nm in Raman out 100 X objec ve (oil immersion) Skin sample Cover glass Brass block Measure along Z- line A well is milled into the brass block
12 Raman Spectra of Materials Raman counts yaluronate prep ylasome prep Ethylhexyl glycerin Pentylene glycol D 2 stretching mode C 2 C 3 stretching region C 2, C 3 scissoring region C 2 twis ng Raman Shi (cm- 1)
13 Raman intensity This skin sec on had ylasome EG10 applied to the top of the SC. Phe ring breathing mode D 2 stretching band Wavenumber (cm- 1) Typical Raman spectrum of surgically removed human skin stratum corneum exposed to 100% Rela ve humidity D 2 Raman intensity Phe ring breathing mode Integrated band area ra o of D 2 / Phe is used to quan fy the rela ve concentra on of D 2 in skin. Skin protein component phenylalanine (Phe), band area is used as an internal standard to correct for sca ering losses with depth. The ra o of the D 2 peak to the Phe peak indicates the moisture content at the measurement site Wavenumber (cm- 1)
14 Applica on in 55% Rela ve umidity D 2 environment D 2 / Phe band area ra o Skin appears to dry over me while exposed to Raman laser. Spectra acquired le to right. A er 24 hour incuba on at 55% Rela ve umidity in a D 2 environment, the results show that the sec on treated with the ylasome prepara on contains more D 2 in the skin and in the stratum corneum than the sec on treated with the 0.1% A formula on. igher ra o numbers indicate higher D 2 concentra on. Depth into skin, µm ylasome EG10 En re sample area Mean ra o: Across skin, µm Depth into skin, µm yaluronic Acid En re sample area Mean ra o: Across skin, µm
15 55% Rela ve umidity D 2 environment D 2 / Phe band area ra o for stratum corneum region using same color scale
16 Conclusion A er 24 hrs at 55% Rela ve umidity: ylasome EG10 treated skin contained 6x more moisture in the total sample and 5x more moisture in the stratum corneum than hyaluronic acid treated skin.
17 Free Radical Scavenger yaluronic Acid (A): Natural scavenger of free radicals (Greenwald & Moy) Protects against free radical- induced cell damage, molecular weight dependent (Pres & Sco 1994) Prevents free radical damage to healing ssue (Foschi, et al 1990) Inhibits lipid peroxida on (Alber ni, et al 2000) ylasome gel: Inhibi on of free radical produc on; free radical consump on (Larsen et al 1992) Accelerates healing of full thickness wounds in rats (SBIR grant final report, 1987, Biomatrix, Inc.)
18 An oxidant Ac vity: Free Radical Scavenging by ylasome [xanthine oxidase/hypoxanthine] Measured in vitro Percent Reduc on of Free Radicals
19 Safety ylasome is : Nonirrita ng Nonsensi zing Noncytotoxic Nonphototoxic Nonhemoly c Nonpyrogenic Nonmutagenic Passes RIPT and Epi- cular Tests.
20 Product Formula on Types Skin Care Creams and Lo ons Skin Care Gels Skin Care Treatment Products Facial Masks Liquid Founda on Make ups Leave- in hair condi oners and styling aids (without ca onic compounds) Recommended Use Level: 1 to 3%
21 Benefits: Five mes the water binding capacity of hyaluronic acid Free radical scavenger Smooth elegant skin feel Slow delivery of water Long Term Moisturiza on Claim
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