Full Product Line. 11. Stabilized Retinol 12. Natural Vitamin C 13. Fermented Oil 14. Anti pollution Agent

Transcription

1 Full Product Line 1. Natural Actives List 2. Natural Preservatives for Cosmetics 3. Natural Anti-Acne Extract 4. Natural Sunscreen 5. Natural Sanitizer 6. Natural Anti-Dandruff Extract 7. Anti-Irritant for Objective Irritation 8. Natural Moisturizer 9. Natural Emulsifier 1. Natural Whitening Agent 11. Stabilized Retinol 12. Natural Vitamin C 13. Fermented Oil 14. Anti pollution Agent

2 Natural Actives List- Ι Group Name Physical test Product Natural Preservative MSE-G3 MSE-G Allium sativum (Garlic) Bulb Extract Camellia Sinensis Leaf Extract Cocos nucifera (Coconut) Oil Yeast Ferment Scutellaria Baicalensis Root Extract Glycyrrhiza Glabra(Licorice)root Extract - Appearance : Pale yellow liquid - ph(2% Solution) : 7.±.5 - Water soluble - Dosage : 1.5%-2.% - Appearance : Pale yellow Liquid - ph: 6. ±.5 - Soluble : in Water(little Turbid) - Application : Natural cosmetics - Dosage : 2% Anti-Acne Extract BCX-CA Chamaecyparis Obtusa Leaf Extract Citrus Unshiu Fruit Extract Glycyrrhiza Glabra(Licorice)root Extract Vitis Vinifera (Grape) Seed Oil - Appearance: Light Brown Liquid - ph: 7. ±.5 - Soluble : in Water - Application : Anti-Acne - Dosage : 2~5% BHC-S Arachis hypogaea fruit Extract Centella asiatica leaf Extract Ecklonia kurome Extract - Appearance : Light Gray Powder - ph : 3.5 ±.5 - Soluble : in Oil - Application : Natural sunscreen formula - Dosage : 3~7% Natural Sunscreen BHC-S(W) Arachis hypogaea fruit Extract Centella asiatica leaf Extract Ecklonia kurome Extract Water Propandiol Scutellaria Baicalensis Root Extract - Appearance : Light Gray Paste - ph : 3.5 ±.5 - Soluble : in Polyol - Application : Natural sunscreen formula. Safe and stable to apply - Dosage : 6~13% Natural Sanitizer MLE-T Glycyrrhiza Glabra(Licorice)Root Extract Camellia Sinensis Leaf Extract - Appearance : Pale Yellow Liquid - ph: 3.5 ±.5 - Soluble : in Water - Application : Hand sanitizer, wet tissue, infants products and anti-microbial spray - Dosage : 2~5% Natural Anti-Dandruff Extract BCX-D Chamaecyparis Obtusa Leaf Extract Cordyceps Militaris Extract - Appearance : Light brown Liquid - ph : 7. ±.5 - Soluble : in Water - Application : Anti-Dandruff - Dosage : 2~5% Natural Anti-Irritant For Objective Irritation Bio Tranquilizer - Water part (BT-W) Solanum tuberosum (Potato) Extract Cordyceps militalis (mycelium) Extract Centella asiatica leaf Extract Magnolia liliflora flower Extract Angelica keiskei leaf/stem Extract - Appearance : Light Brown Liquid - ph : 5.5 ±.5 - Soluble : in Water - Application : Anti-inflammation, Anti-Allergy, Anti-oxidant - Dosage : 1~2%

3 Natural Actives List- ΙΙ Group Name Physical test Product Natural Moisturizer BST- Mucohydra BST- Physiological Lipid(PL) Glycosaminoglycans Glycyrrhiza Glabra(Licorice)Root Extract α-linolenic acid Phosphatidyl Choline Sodium Hyaluronate Water -Appearance :Colorless and Transparent Liquid - ph(1% Solution) : 3.5±.5 - Soluble : in Water - Application : Moisturizing - Dosage : 5~7% - Appearance : Light Yellow Semisolid - ph(1% Solution) : 6.3±.5 - Soluble : in Water - Application : Moisturizing - Dosage : 1~5% Natural Emulsifier BST- BioEmulsifier Hydrogenated lecithin C12-16 Alcohols Palmitic acid -Appearance : Light Yellow Powder - ph(2% Solution) : 4.5±.5 - Soluble : in Water - Application : Natural Cosmetics - Dosage : 1~5% Natural Whitening Agent BST- Seedwhiter Niacinamide( Vit B3) Citrus junos seed Extract Scutellaria Baicalensis Root Extract -Appearance: Whiter Powder - ph(2% Solution) : 5.5±.5 - Soluble: in Water - Application: Natural Cosmetics - Dosage : 2~4% Stabilized Retinol BST-Retinol Lecithin Retinol Water - Appearance : Orange liquid - ph(2% Solution) : 6.±.5 - Solubility : Water soluble (Can be added in water phase) - Dosage : 2% Natural Vitamin C BST-Natural Ascorbic Acid Malpighia Emarginata(Acerola) fruit Extract Butylene Glycol - Appearance : Brown liquid - ph(2% Solution) : 3.5±.5 - Solubility : in Water - Application: Natural Cosmetics - Dosage : 1~2% Ferment Oil BST- Ferment Oil Camellia Japonica Seed Oil ph : 5.±.5 Linum Usitatissimum (Linseed) Seed Oil ph : 4.±.5 Olive Oil ph : 4.±.5 Anti pollution Agent BST-CR (Cell Recovery) Cordyceps Sinensis Extract (β-glucan) Caulerpa Lentillifera Extract (Flavonoid) Pinus Densiflora Bark Extract (Proanthocyanidin) Propanediol - Appearance : Light Brown Liquid - ph(2% Solution) : 3.8±.5 - Solubility : in Water - Dosage : 2% Natural Anti-Foa ming BST-AF Olive Oil Soybean oil Soybean Lecithin Sucrose fatty acid ester - Appearance : yellow liquid - ph : 5.7 ±.5 - Solubility : in Water - Dosage : 2%

4 Natural Preservatives for Cosmetics A natural preservative has been constituted of purified fractions of traditional oriental herb which has medicinal effects such as anti-microbial, anti-inflammation, fever remedy, antioxidation, and anti-aging effect etc. MSE-G Scutellaria Baicalensis Root Extract Glycyrrhiza Glabra(Licorice)root Extract - Appearance : Pale Yellow Liquid - ph : 6. ±.5 - Soluble : in Water (Little Turbid) - Application : Natural cosmetics - Dosage : 2% MSE-G3(edible) Allium Sativum(Garlic) Bulb Extract Camellia Sinensis Leaf Extract Cocos Nucifera(Coconut) Oil Yeast Ferment - Appearance : Pale Yellow Liquid - ph : 7. ±.5 - Soluble : In Water - Application : Natural Cosmetics, Food - Dosage : 1.5~2.% Function 1. Natural and applied to all kinds of cosmetics 2. Broad scope of anti-microbial spectrum (bacteria and fungi) 3. Not affective to viscosity 4. No action to macromolecule. Applied to any products 5. Upgraded in water phase, sensual property, and anti-fungi MIC Test Strains MSE-G(%) MSE-G3(%) 1,2- Hexanediol(%) EHG (%) S. aureus E.coli P.aeruginosa C.albicans A.niger

5 Natural Anti-Acne Extract BCX-CA Chamaecyparis Obtusa Leaf Extract, Citrus Unshiu Fruit Extract, Glycyrrhiza Glabra(Licorice)root Extract Vitis Vinifera (Grape) Seed Oil, - Appearance: Light Brown Liquid - ph: 7. ±.5 - Soluble: in Water - Application: BCX-CA has better efficacy than tea tree oil, which was proved by clinical trial. - Dosage : 2~5% Ingredient / Function Chamaecyparis obtusa Leaf Extracts - Revolutionary natural ingredient that has been proven to dramatically improve acne conditions by clinical study - Inhibits the growth of P. acnes - Reduces and controls blocked pores more effective than tea tree oil, BCX-CA vs tea tree oil - BCX-CA inhibits P.acnes more effectively than tea tree oil 1 : Tea tree oil 2 : Natural extract of Citrus Unshiu peel 3 : BCX-CA (most wide clear zone in BCX-CA compound ) Active ingredients increased after fermentation 1 Dihydrobenzoic acid (Anti-microbial effect) 2 Taxifolin 3-O-β-D-xylopyranosid (=flavone : Anti-bacteria, Anti-oxidation ) 3 Quercetin 3-rhamnoside (= flavone) Peaks were increased after fermentation.

6 Conc. (μl/ml) O.D BCX-CA vs. tea tree oil and fermentation 1. BCX-CA 2. tea tree oil 3. natural extract of Citrus Unshiu peel Growth inhibition of P. acnes : 24hrs incubation % of BCX-CA inhibited the growth of P. acnes more than 5% of tea tree oil tea tree 5% MCE-CA BCX-CA 5% 5% before bioconverted control Anti-inflammation efficacy 4 TNF-α 2 (+) (-) BCX-CA(.1) MCE-CA(.1) MCE-CA(.5) BCX-CA(.5) MCE-CA(.1) BCX-CA(.1) TNF- α decreased, When BCX-CA was added to LPS irritated HaCAT cell. Clinical study : performed at the Seoul National University Hospital Baseline After 8 weeks

7 Natural Sunscreen BHC-S Arachis hypogaea fruit extract, Centella asiatica leaf extract, Ecklonia kurome extract - Appearance : Light Gray Powder - ph : 3.5±.5 - Soluble : W/O, O/W (biphasic) - Application : Natural sunscreen formula (equal to OMC) - Dosage : 3~7% Antioxidation Antiinflammation ㆍ Centella asiatica extract Sunscreen ㆍ Arachis hypogaea fruit extract ㆍ Ecklonia kurome extract UV sensitive yeast strain_bio assay (2mg/L) Absorption spectrum (1mg/L) OMC BHC-S 1. BHC-S 2. Ethlhexyl Methoxycinnamate (OMC) Control 1. BHC-S strongly protects the UV sensitive yeast strain and absorbs broad UV spectrum 2. It can be easily incorporated into any formulation 3. In combination with other UV-filters, high SPF can be achieved. BST lnc. Tel Fax s biosales@ibst.kr

8 Natural Sanitizing Ingredient MLE-T Glycyrrhiza Glabra(Licorice) Root Extract, Camellia Sinensis Leaf Extract, - Appearance: Pale Yellow Liquid - ph: 3.5 ±.5 - Soluble: in Water - Application: For hand sanitizer (edible ingredient), wet tissue, infants products and anti-microbial spray - Dosage : 2~5% Anti-bacterial efficacy - Sample : 5% solution of MLE-T, 1% solution of MLE-T - Test Time : 5min Sample Antibacterial efficiency(%) S. aureus E. coli S. typhiurium A.niger P.pinophilum 5% MLE-T % MLE-T Anti-bacterial activity rate against pathogenic microbe Control 7% Alcohol 3% MLE-T - Sample : 3% MLE-T solution - Test subjects : Nonwovens - Strains : Aspergillus niger, Penicillium pinophilum, Chaetomium globosum, Gliocladium virens, Aureobasidium pullulans. - Test result : No Growth Bioassay compared to alcohol MLE-T MLE-T MLE-T MLE-T Ethanol Isopropyl alcohol Ethanol Isopropyl alcohol Ethanol Isopropyl alcohol Ethanol Isopropyl alcohol S.aureus E. coli C.albicans A.niger

9 O.D BCX-D Chamaecyparis Obtusa Leaf Extract, Cordyceps Militaris Extract - Appearance: Light Brown Liquid - ph : 7. ±.5 - Soluble: in Water - Application: BCX-D is Natural Anti-Dandruff ingredient made using Chamaecyparis obtusa and cordyceps - Dosage : 2~5% BCX-D vs. hinoki oil & zinc pyrithione 1: BCX-D 2. Hinoki oil 1.BCX-D 2. zinc pyrithione Bio-conversion effect Efficacy of active ingredients of extracts of Chamaecyparis obtusa leaf & Cordyceps sinensis was maximized by fermentation, which increases the inhibition of P.ovale. 1: Before bio-conversion 2: After bio-conversion Growth inhibition of P. ovale after 3 days incubation % of BCX-D inhibited the growth of P. ovale more than 5% of hinoki oil and inhibition rate of BCX-D and zinc pyrithione was almost same. hinoki oil 5% BCX-D 5% zinc before control pyrithione 5% bioconverted

10 IL-6 (pg/ml) TNF-α (pg/ml) NO production (% of control) IL-1ß(pg/ml) Anti-Irritant for Objective Irritation Bio Tranquilizer BT-W Solanum Tuberosum (Potato) Extract, Cordyceps Militalis Mycelium Extract, Centella Asiatica Leaf Extract, Magnolia Liliflora Flower Extract, Angelica Keiskei Leaf/Stem Extract - Appearance : Light Brown Liquid - ph : 5.5 ±.5 - Soluble : in Water - Application : Anti-inflammation, Anti-allergy, Anti-oxidation - Dosage : 1~2% Effect : Anti-inflammation, Anti-allergy, Anti-ithcing, Anti-irritation NO inhibition assay (RAW cell) Anti-Inflammation IL-1ß (RAW cell) control Concentration of sample (% of stock solution) control Concentration of sample (% of stock solution) Anti-Inflammation IL-6 (RAW cell) Tumor Necrosis Factor (RAW cell) control Concentration of sample (% of stock solution) Concentration of sample (% of stock solution)

11 Electric conductivity(μ/ω) DPPH scavening activity (%) Natural Moisturizer BST Mucohydra, the skin-moisturizing ingredients containing Glycosaminoglycans, is made of Korean snail slime extract fermented with vegetable lactobacillus. It is a multifunctional moisturizing ingredient, which shows rapid moisture absorption and excellent moisturizing effect than normal snail slime extract and has skin protection effect from antioxidant activity. BST-Mucohydra Glycosaminoglycans Glycyrrhiza Glabra(Licorice) Root Extract - Appearance : Colorless and Transparent Liquid - ph(1% Solution) : 3.5±.5 - Water solubility : Soluble - Application: Moisturizer - Dosage : 5~7% Type Diluted type : instead of water(dosage : 5~95%) Concentrated type : functional(dosage : 2%) Effect Moisturizing activity Moisture retention is better than common snail slime extract and green tea extract. Antioxidant activity Antioxidant activity is dependent on the concentration of BST-Mucohydra. Skin conductance test Antioxidant activity test Base cream hr 1hr 2hr Cream with BST Physiological lipid 2% control Concentration (%) Application All type of formulations for sensitive skin Protective day care products, especially for irritated or stressed skin

12 Permeation profiles of BST-Mucohydra and Snail secretion filtrate (ug/cm 3 ) DPPH scavening activity (%) Natural Moisturizer BST- Physiological lipid is a moisturizing ingredient. It is a liposome formed a lipid complex which consists of α-linolenic acid(omega-3) and Phosphatidyl choline replacing physiological lipid that function as Skin protection. Especially this liposome is collected low molecular hyaluronic Acid which performs moisture storage of dermal layer. It can make functional ingredients pass through the skin deeply. BST- Physiological lipid α-linolenic acid Phosphatidyl Choline(PC) Sodium Hyaluronate Water -Appearance : Light Yellow Semisolid - ph (1% Solution) : 6.3±.5 - Water solubility : Soluble (little Turbid) - Application : Moisturizer - Dosage : 1~5% 1 Quality BST-Physiological lipid(bst-pl) consists of EDIBLE INGREDIENTS. EWG S rating of All the ingredients is below 2. Effect Natural moisturizing ingredients Physiological lipid, the essential ingredient, which function as Skin protection Rich in omega-3 fatty acids Containing low molecular weight hyaluronic Acid, function as moisture storage of dermal layer TEWL Skin permeation test homogenization Antioxidant activity test BST-PL HA Basic cream hr 2hr 6hr BST lnc. Cream with BST Physiological lipid 2% Time (hr) Time (hr) Concentration (%) Tel Fax biosales@ibst.kr

13 Natural Emulsifier Natural Lamellar Emulsifier BST-bioemulsifier Palmitic acid C12-16 Alcohols Hydrogenated lecithin - Appearance : Light yellow powder - ph(2% Solution) : 4.5±.5 - Water solubility : Soluble - Application: Natural Cosmetics - Dosage : 1~5% - Phospholipids are characterized by low HLB values poor emulsifying agents. Their emulsifying properties are enhanced in combination with certain fatty acids and fatty alcohols - Form lamellar network - EWG S rating of All the ingredients is below 2. Product application BST-Bioemulsifier H water water BST-Bioemulsifier L L :Low Viscosity type with lotion H:High Viscosity type with cream Effect - Natural surfactants made with Lecithin extracted from soy beans - Having excellent skin affinity than other natural surfactants - Anti-aging by containing phytogenic peptide and phosphatidyl choline - Moisturizing activity

14 Anti oxidation rate (%) Natural Whitening Agent BST-Seedwhiter Scutellaria Baicalensis Root Extract Citrus junos seed Extract Niacinamide( Vit B3) - Appearance : Light yellow powder - ph(2% Solution) : 5.5±.5 - Solubility : in Water - Application : Natural Cosmetics - Dosage : 2~4% -BST-Seedwhiter is a whitening ingredients combined Lactobacillus ferment with Scutellaria Baicalensis Root Extract and Citrus junos seed Extract. It can replace Arbutin which has an issue about Formaldehyde. -EWG S rating of All the ingredients is 1. BST-Seedwhiter Effect Multi-Functional whitening activity Inhibit reactive oxygen&melanin synthesis, transport, and signal cascade Antioxidant activity Experiments show that, Bio-whiter has good efficacy on free radical scavenging, at 1ug/ml concentration anti-oxidation rate reach 94% or more, even better than the anti-oxidation effect of 1ug/ml arbutin Tyrosinase inhibition activity BST-Whiter on the activity of tyrosinase inhibition is more stronger than arbutin At the result of Whitening active measurement using melanoma cell line, BST-Seedwhiter have shown tyrosinase inhibitory activity. Protection of environmental pollutions Protect to environmental pollutions such as UV, and the other stress factors involved in tyrosinase activation DPPH radical scavenging Arbutin 1ug/ml BIO-Whiter 1ug/ml Tyrosinase inhibition activity (mg/ml) Concentration of BST-SeedWhiter

15 Stabilized Retinol BST-Retinol (15,5 IU/g) Lecithin Retinol Water - Appearance : Orange liquid - ph(2% Solution) : 6.±.5 - Solubility : Water soluble (Can be added in water phase) - Dosage : 2% BST-Retinol is a material that stabilized retinol through liposome process. It is possible to be stable to heat and light and add to water compared with pure retinol. Also, Lecithin extracted from bean contains a lot of flavonoid ingredients and provides a synergy effect in complex action with retinol.. Manufacturing Process retinol Liposome formation homogenization Microfluidizer Retinolsome Stability test Antioxidative effect of retinol is confirmed after storing for 3 months exposing to sunlight. Appearance Change Retinol BST- Retinol Measurement of Antioxidative activity (DPPH) Layer separation, sedimentation, and etc after 3months Retinol BST- Retinol were not found at all. As a result of antioxidative test, BST-Retinolsome showed more outstanding antioxidative activity than pure Effect retinol. Antioxidant activity BST-Retinol has good efficacy on free radical scavenging. Antiaging activity The strongest antiaging material affecting the hindrance of collagen decomposition enzyme and cell proliferation.

16 Natural Vitamin C BST-Natural Ascorbic Acid Malpighia Emarginata (Acerola) Fruit Extract Butylene Glycol - Appearance : Brown liquid - ph(2% Solution) : 3.5±.5 - Water solubility : Soluble - Application: Natural Cosmetics - Dosage : 2% - Acerola contains 2~3 times more vitamin C than Lemon. - Extract contains various physiological activity materials such as polyphenol compound in addition to vitamin C. - Natural Vitamin C is 1.5~2 times higher in absorptivity than synthetic vitamin C. Effect Antioxidant activity Acerola - RC5 value of vitamin C is 1.3 μg / ml and this shows efficacy similar to vitamin C. After 6 month Anti-inflammation In case of comparing with vitamin C known for anti-inflammatory efficacy, Acerola Extract (Vit.C) is thought to show similar efficacy. Multi-Functional whitening activity Inhibit reactive oxygen & melanin synthesis, transport, and signal cascade Skin protection from active oxygen from UV and block UV rays Improves cell activity by helping the regeneration of skin cell.

17 % Antioxidant activity Nitrite(mM) BST- Ferment Oil INCI Image Material Benefits ph Story Camellia Japonica Seed Oil -Gamma Linolenic acid -Triglycerides -Oleic acid -Moisturizing -Antioxidant activity -Conditioning effect - Improvement of skin irritation 5.±.5 -Camellias were cultivated in the gardens of China and Japan for centuries. -Camellia oil and it is used heavily in cosmetic applications. Linum Usitatissimum (Linseed) Seed Oil -Omega-3 -Lignans -Dietary fiber -Atopic dermatitis suppression -Melasma, freckles effect -Improve skin problems 4.±.5 -Flax is one of the English-grown medicinal herbs. -Seed known as Linseed, being much employed in medicine. Olive Oil -Oleic acid -Vitamin E -Linolenic acid -Moisturizing -Anti-aging -Antioxidant activity -Protection against UVB damage -Anti-inflammation 4.±.5 -Olive oil is used in traditional Medicine and food from 6, years ago. -Olive oil used for Ferment oil. -Olive is grown with fertile soil and rich sunlight of Italy. Natural Oil Ferment-purification Ferment strains -Candida spp : Isolated from soy Ext. -Lactobacillus spp : Isolated from milk Ext. Ferment process 1. seed cultures (lipase induction) (oil seed) + (strains media) 2. Cellulose bead immobilization of lipase broth 3. Fermentation immobilized lipase + oil 4. Centrifugation ( 1, rpm, 2min) 5. Recovery Ferment oil BST- Ferment Oil -Anti-oxidation -Anti-browning -Emulsifying capacity -Skin absorption -Non-greasy Feeling Immobilize Fermentation Purification Ferment Oil Vit E 1 Camellia Camellia 1% 1% +Ferment Linum 1% Linum 1% + Ferment Olive 1% Olive 1% + Ferment con LPS Camellia Camellia 1% 1% +Ferment Linum 1% Linum 1% + Ferment Olive 1% Olive 1% + Ferment Anti-Oxidative Effect DPPH assay +LPS Inhibition of LPS-induced production of Nitric Oxide

18 BST-CR (Cell Recovery) It is an ingredient that protects skin cell skin from metal, carbon origin, and ultrafine particles by containing Cordyceps Sinesis, β-glucan, sea grapes that contains Flabonoid, and Pine Bark that contains Proanthocyanidin. BST-CR (Cell Recovery) Cordyceps Sinensis Extract (β-glucan) Caulerpa Lentillifera Extract (Flavonoid) Pinus Densiflora Bark Extract (Proanthocyanidin) Propanediol Physical test - Appearance : Light Brown Liquid - ph(2% Solution) : 3.8±.5 - Solubility : in Water - Dosage : 2% Manufacturing Process Betaglucan (from cordyceps militaris) Conversion through Fermentation (changing High Molecular to Low Molecular) How It Becomes Low Molecular Refinement of Regenerating Active Ingredients Caulerpa Lentillifera Extract (Flavonoid) Pinus Densiflora Bark Extract (Proanthocyanidin) Hot Water Extraction Filtering And Refining BST-CR

19 Relative expression (Fold change 1%) Relative expression (Fold change 1%) DPPH radical scavenging effect (%) Cell proliferation (%) BST-CR (Cell Recovery) Effect Antioxidant Activity DPPH(α,α-diphenyl-β-picrylhydrazyl) is a free radical that is chemically stabilized and when it meets an substance that has antioxidant activity, this substance gives electron to the radical so it is eliminated. So, the color changes. By testing 3 substances, BST-CR had bigger DPPH radical scavenging activity than O* (.1% solution).. 1 DPPH radical scavenging activity Skin Protection Effect After dividing HaCaT Cell which is an epidermal cell in 1*1^4 concentration to 96well plate per well, it was cultured by 24hours. After this step, ultrafine particles solution was put in. By confirming cell proliferation of untreated group, Beta Glucan treated group, and BST-CR treated group, BST-CR showed more than 2 times better effect than untreated group Vit.C (.1%) O* (.1%) BST-CR (.1%) Cytokine Regulatory Effects By putting ultrafine particles solution that contains heavy metal to HaCaT Cell that is an epidermal cell, it was left for 24 hours. Then, Potassium Phosphate Saline Buffer was put in and went through homogenization. By doing so, RT-PCR was conducted dividing RNA. After Reverse Transcription through Reverse Transcriptase, reaction of PCR was observed in each IL-1 and IL-1. In results, it was obvious to see that BST-CR had less cytokine than Caulerpa Lentillifera Extract or Beta Glucan. 2 con Beta glucan BST-CR IL-1 IL-1 Cell Regenerating Effects After wounding HaCat Cell which is an epidermal cell, it was divided into untreated group and BST-CR which is a treated group. After 48 hours, cytothesis effect of untreated group and BST-CR was compared. All in all, BST-CR which is a treated group had more than 5% of regenerating effects than untreated group. con BST-CR Vehicle BST-CR

20 BST-AF (Natural Anti-Foaming) Olea Europaea (Olive) Fruit Oil Soybean Oil Lecithin Sucorose Fatty Acid Ester Natural Antifoaming Agent by emulsifying 1% natural oil with lecithin that is free silicon, alcohol, and mineral salt. Having antioxidant effect and extra effects compared to existing defoamingagent that is free alkali silicon. Moreover, it went through emulsifying process and MF process from olive oil and flaxseed oil which has an abundant flavor. Our Test Feature Appearance yellow liquid ph 5.7 ±.5 Control A company BST Dosage.1% ~.3% prescription is recommended for foam manufacturing